Selection of antibiotic resistance by metals in a riverine bacterial community

Silva, Isabel; Tacão, Marta; Henriques, Isabel

doi:10.1016/j.chemosphere.2020.127936

Cited by 29 publications

(19 citation statements)

References 39 publications

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“…A major stability and persistence of heavy metals relative to antibiotics, which are more rapidly degraded in the environment, may constitute a long-term selective pressure enhancing AMR. In riverine microcosms, exposure to 50 and 100 μg L –1 of zinc or copper correlated with increased prevalence of bacteria intrinsically resistant to cefotaxime and kanamycin (Silva, Tacão and Henriques 2021 ). A paleontology study of pond sediments found that Zn concentrations and bioavailability correlated with the level of Zn tolerance, resistance to clinically relevant antibiotics and composition of culturable microbial isolates (Dickinson et al .…”

Section: Amr and Pressure From Antibiotic Metabolites And Heavy Metalsmentioning

confidence: 99%

Knowledge gaps in the assessment of antimicrobial resistance in surface waters

Niegowska

Sanseverino

Navarro

et al. 2021

FEMS Microbiology Ecology

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The spread of antibiotic resistance in the water environment has been widely described. However, still many knowledge gaps exist regarding the selection pressure from antibiotics, heavy metals and other substances present in surface waters as a result of anthropogenic activities, as well as the extent and impact of this phenomenon on aquatic organisms and humans. In particular, the relationship between environmental concentrations of antibiotics and the acquisition of ARGs by antibiotic-sensitive bacteria as well as the impact of heavy metals and other selective agents on antimicrobial resistance (AMR) need to be defined. Currently, established safety values are based on the effects of antibiotic toxicity neglecting the question of AMR spread. In turn, risk assessment of antibiotics in waterbodies remains a complex question implicating multiple variables and unknowns reinforced by the lack of harmonized protocols and official guidelines. In the present review, we discussed current state-of-the-art and the knowledge gaps related to pressure exerted by antibiotics and heavy metals on aquatic environments and their relationship to the spread of AMR. Along with this latter, we reflected on i) the risk assessment in surface waters, ii) selective pressures contributing to its transfer and propagation, and iii) the advantages of metagenomics in investigating AMR. Furthermore, the role of microplastics in co-selection for metal and antibiotic resistance, together with the need for more studies in freshwater are highlighted.

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Section: Amr and Pressure From Antibiotic Metabolites And Heavy Metalsmentioning

confidence: 99%

Knowledge gaps in the assessment of antimicrobial resistance in surface waters

Niegowska

Sanseverino

Navarro

et al. 2021

FEMS Microbiology Ecology

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“…This assertion is verified by Silva [ 82 ] who demonstrates by qPCR, that the richness of antibiotic resistance genes in the copper-exposed bacterial focus is not higher than in the unexposed control microcosm. Indeed, the absolute abundance of these genes, such as blaCTX-M and blaTEM (genes encoded for an extended-spectrum betalactamase), was below the limit of detection.…”

Section: Copper Selective Pressure and Resistancesmentioning

confidence: 57%

“…This phenomenon is known as cross-resistance ( Figure 2 a). For example, in the study conducted by Silva [ 82 ], in a river bacterial community exposed to copper concentrations between 50 and 100 µg/L (corresponding to environmentally relevant concentrations), the most represented genus showing resistance to cefotaxime was Pseudomonas . Teixeira [ 83 ] demonstrated that in Pa , the co-selection mechanism of cefotaxime and copper resistance is largely due to cross-resistance involving efflux pumps ( Figure 2 a).…”

Section: Copper Selective Pressure and Resistancesmentioning

confidence: 99%

From Copper Tolerance to Resistance in Pseudomonas aeruginosa towards Patho-Adaptation and Hospital Success

Virieux-Petit

Hammer-Dedet

Aujoulat

et al. 2022

Genes

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The hospital environment constitutes a reservoir of opportunistic pathogens responsible for healthcare-associated infections (HCAI) such as Pseudomonas aeruginosa (Pa). Pa persistence within technological niches, the increasing emergence of epidemic high-risk clones in HCAI, the epidemiological link between plumbing strains and clinical strains, make it a major nosocomial pathogen. Therefore, understanding the mechanisms of Pa adaptation to hospital water systems would be useful in preventing HCAI. This review deciphers how copper resistance contributes to Pa adaptation and persistence in a hospital environment, especially within copper water systems, and ultimately to its success as a causative agent of HCAI. Numerous factors are involved in copper homeostasis in Pa, among which active efflux conferring copper tolerance, and copper-binding proteins regulating the copper compartmentalization between periplasm and cytoplasm. The functional harmony of copper homeostasis is regulated by several transcriptional regulators. The genomic island GI-7 appeared as especially responsible for the copper resistance in Pa. Mechanisms of copper and antibiotic cross-resistance and co-resistance are also identified, with potential co-regulation processes between them. Finally, copper resistance of Pa confers selective advantages in colonizing and persisting in hospital environments but also appears as an asset at the host/pathogen interface that helps in HCAI occurrence.

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“…Reporting in 2011 a 200-fold increase of the relative abundances of six antibiotic-resistance genes (including sul1 ) as compared to sediment sampled in the center of the lake, Czekalski et al (2014) hypothesized that the increasing abundance of antibiotic-resistance genes was mainly due to direct inputs from wastewater effluents rather than in situ development of antibiotic-resistant bacteria in the lake sediment following their chronic exposure to contaminants. In the present study, given the high metal concentrations observed in the surface sediment from the Vidy Bay, the hypothesis of co-selection of antibiotic resistance by this kind of contaminant cannot be excluded ( Poole, 2017 ; Silva et al, 2021 ). Although pharmaceutical residues in the collected sediments were not analyzed it is more than probable that site 53 sediment were contaminated by antibiotics ( Morasch et al, 2010 ; Bonvin et al, 2011 , 2013 ), potentially favoring the development of antibiotic resistance in sediment communities in response to antibiotic selection pressure ( Bengtsson-Palme and Larsson, 2016 ).…”

Section: Discussionmentioning

confidence: 70%

Diversity, Functions and Antibiotic Resistance of Sediment Microbial Communities From Lake Geneva Are Driven by the Spatial Distribution of Anthropogenic Contamination

et al. 2021

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Lake sediments are natural receptors for a wide range of anthropogenic contaminants including organic matter and toxicants such as trace metals, polycyclic aromatic hydrocarbons, polychlorinated biphenyls that accumulate over time. This contamination can impact benthic communities, including microorganisms which play a crucial role in biogeochemical cycling and food-webs. The present survey aimed at exploring whether anthropogenic contamination, at a large lake scale, can influence the diversity, structure and functions of microbial communities associated to surface sediment, as well as their genetic potential for resistance to metals and antibiotics. Changes in the characteristics of these communities were assessed in surface sediments collected in Lake Geneva from eight sampling sites in October 2017 and May 2018. These sampling sites were characterized by a large concentration range of metal and organic compound contamination. Variation between the two sampling periods were very limited for all sampling sites and measured microbial parameters. In contrast, spatial variations were observed, with two sites being distinct from each other, and from the other six sites. Benthic communities from the most contaminated sampling site (Vidy Bay, near the city of Lausanne) were characterized by the lowest bacterial and archaeal diversity, a distinct community composition, the highest abundance of antibiotic resistance genes and functional (respiration, denitrification, methanogenesis, phosphatase, and beta-glucosidase) activity levels. The second sampling site which is highly influenced by inputs from the Rhône River, exhibited low levels of diversity, a distinct community composition, high abundance of antibiotic resistance genes and the highest bacterial abundance. Overall, our results suggest that local anthropogenic contamination, including organic matter and toxicants, is a major driver of the diversity and functioning of sediment-microbial communities in Lake Geneva. This highlights the need to consider benthic microbial communities and a suite of complementary ecotoxicological endpoints for more effective environmental risk assessments of contaminants in lake sediments.

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Selection of antibiotic resistance by metals in a riverine bacterial community

Cited by 29 publications

References 39 publications

Knowledge gaps in the assessment of antimicrobial resistance in surface waters

Knowledge gaps in the assessment of antimicrobial resistance in surface waters

From Copper Tolerance to Resistance in Pseudomonas aeruginosa towards Patho-Adaptation and Hospital Success

Diversity, Functions and Antibiotic Resistance of Sediment Microbial Communities From Lake Geneva Are Driven by the Spatial Distribution of Anthropogenic Contamination

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