Selection of aptamers for fluorescent detection of alpha-methylacyl-CoA racemase by single-bead SELEX

Yang, Deng-Kai; Chen, Lin-Chi; Lee, Ming-Ying; Hsu, Chun‐Hua; Chen, Chun-Shen

doi:10.1016/j.bios.2014.06.027

Cited by 25 publications

(14 citation statements)

References 34 publications

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“…The most frequently used supports are microbeads, 51–53 which are easily retained by a microcolumn filter during selection, but other surfaces such as microplates are also used. 54, 55 In both cases, the target can be attached covalently with linker chemistry, 56 adsorbed non-specifically, 54 or linked with biotin to a streptavidin-coated surface. 57 While this technique enhances flexibility and the number of allowable targets, macroscale affinity chromatography on the whole does not substantially increase the efficiency of the selection and often uses relatively large amounts of material.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic methods for aptamer selection and characterization

Dembowski

Bowser

2018

Analyst

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Nucleic acid aptamers have tremendous potential as molecular recognition elements in biomedical targeting, analytical arrays, and self-signaling sensors. However, practical limitations and inefficiencies in the process of selecting novel aptamers (SELEX) have hampered widespread adoption of aptamer technologies. Many factors have recently contributed to more effective aptamer screening, but no influence has done more to increase the efficiency, scale, and automation of aptamer selection than that of new microfluidic SELEX techniques. This review introduces aptamers as a powerful chemical and biological tool, briefly highlights traditional SELEX techniques and their limitations, covers in detail the recent advancements in microfluidic methods of aptamer selection and characterization, and suggests possible future directions of the field.

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Section: Introductionmentioning

confidence: 99%

Microfluidic methods for aptamer selection and characterization

Dembowski

Bowser

2018

Analyst

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“…Among the various qPCR systems, fluorescence-based detection technologies are the most advanced and can feature either an intercalating fluorescent dye (e.g., SYBE Green I) or a fluorescent probe (e.g., molecular beacon). 25,26 However, real-time qPCR instruments are expensive and large. Therefore, developing a portable qPCR instrument is a critical research focus.…”

Section: Introductionmentioning

confidence: 99%

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Hung

Ho²,

Chen

2016

J. Biomed. Opt

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This study developed a microfluidic platform for replicating and detecting DNA in real time by integrating a laser and a microfluidic device composed of polydimethylsiloxane. The design of the microchannels consisted of a laser-heating area and a detection area. An infrared laser was used as the heating source for DNA replication, and the laser power was adjusted to heat the solutions directly. In addition, strong biotin–avidin binding was used to capture and detect the replicated products. The biotin on one end was bound to avidin and anchored to the surface of the microchannels, whereas the biotin on the other end was bound to the quantum dots (Qdots). The results showed that the fluorescent intensity of the Qdots bound to the replicated products in the detection area increased with the number of thermal cycles created by the laser. When the number of thermal cycles was ≥10, the fluorescent intensity of the Qdots was directly detectable on the surface of the microchannels. The proposed method is more sensitive than detection methods entailing gel electrophoresis.

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“…Type and purity of the targets are also effective subjects on the SELEX outcome. The accuracy of the method is dependent on the election of the initial libraries so that the incomplete libraries cause the loss of the aptamers with the highest target specificity (Blind & Blank, 2015;Darmostuk et al, 2015;D.-K. Yang et al, 2014;Yazdian-Robati et al, 2019;C. Zhang et al, 2014).…”

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confidence: 99%

Recent advances in computational methods for biosensor design

Khoshbin

Housaindokht

Izadyar

et al. 2020

Biotech & Bioengineering

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Biosensors are analytical tools with a great application in healthcare, food quality control, and environmental monitoring. They are of considerable interest to be designed by using cost‐effective and efficient approaches. Designing biosensors with improved functionality or application in new target detection has been converted to a fast‐growing field of biomedicine and biotechnology branches. Experimental efforts have led to valuable successes in the field of biosensor design; however, some deficiencies restrict their utilization for this purpose. Computational design of biosensors is introduced as a promising key to eliminate the gap. A set of reliable structure prediction of the biosensor segments, their stability, and accurate descriptors of molecular interactions are required to computationally design biosensors. In this review, we provide a comprehensive insight into the progress of computational methods to guide the design and development of biosensors, including molecular dynamics simulation, quantum mechanics calculations, molecular docking, virtual screening, and a combination of them as the hybrid methodologies. By relying on the recent advances in the computational methods, an opportunity emerged for them to be complementary or an alternative to the experimental methods in the field of biosensor design.

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Selection of aptamers for fluorescent detection of alpha-methylacyl-CoA racemase by single-bead SELEX

Cited by 25 publications

References 34 publications

Microfluidic methods for aptamer selection and characterization

Microfluidic methods for aptamer selection and characterization

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Recent advances in computational methods for biosensor design

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