Selection of Beauveria isolates pathogenic to adults of Nilaparvata lugens

Abstract

The brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae), is a destructive invasive pest and has become one of the most economically-important rice pests in China. Effective control measures are desperately needed. Entomopathogenic fungi, such as Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Clavicipitaceae) and B. brongniartii (Saccardo), have shown great potential for the management of some sucking pest species. In this study, to explore alternative strategies for sustainable control of the sucking pest population, nine isolates of Beauveria from different pests were bioassayed under the concentrated standard spray of 1000 conidia/mm 2 in laboratory. The cumulative mortalities of adults ranged from 17.2 to 79.1% 10 days after inoculation. The virulence among all tested isolates exhibited significant differences (at p = 0.05). The highest virulent isolate was Bb09, which killed 79.1% of the treated insects and had a median lethal time of 5.5 days. Its median lethal concentration values were estimated as 134 conidia/mm 2 on day 10. The chitinase activities of nine isolates were also assayed. The results showed that the chitinase activity (18.7 U/mg) of isolate Bbr09 was the highest among all tested isolates. The biological characteristics of these strains, including growth rate, sporulation, and germination rate, were further investigated. The results showed that strain Bbr09 exhibited the best biological characteristics with relatively higher hyphal growth rate, the highest spore production, and the fastest spore germination. The isolate of Bbr09 had strong pathogenicity and exhibited great potential for sustainable control of N . lugens .

Susceptibility of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae), a Eucalyptus pest, to entomopathogenic fungi

“…This would reduce the possible detrimental effects of fungi on parasitoid development. Susceptibility of T. peregrinus to the mycoinsecticides B. bassiana and M. anisopliae is similar to that reported for other Hemiptera pests, such as Nilaparvata lugens Stål (Hemiptera: Delphacidae) (Li et al, 2014), Diaphorina citri Kuwayama (Hemiptera: Liviidae) (Orduño-Cruz et al, 2015), Nezara viridula Linnaeus (Hemiptera: Pentatomidae) (Raafat et al, 2015), Aeneolamia spp. (Hemiptera: Cercopidae) (Hernández-Domínguez et al, 2016) and Glycaspis brimblecombei (Dal Pogetto et al, 2011a;20011b).…”

“…Damage by T. peregrinus in eucalyptus plantations and the need for products that comply with forest certification requirements Lemes et al, 2017) show the importance of entomopathogenic fungi for the management of this pest. These studies are significant because B. bassiana and M. anisopliae caused 79 % mortality of adults of N. lugens 10 days after inoculation (Li et al, 2014) and 50 and 60 % mortality, respectively, to D. citri (Lezama-Gutiérrez et al, 2012). Other pathogenic fungi for these insects include Hirsutella citriformis Kuwayama (Ascomycota: Hypocreales: Ophiocordyceps) with 100 % mortality for D. citri (Orduño-Cruz et al, 2015).…”

MicroRNA and dsRNA targeting chitin synthase A reveal a great potential for pest management of the hemipteran insectNilaparvata lugens

“…This method has been successfully applied for gene knockdown via ingestion in several pest insects, including S. exigua and Leptinotarsa decemlineata . Unlike symbiotic fungi, the entomopathogenic fungi, such as Beauveria and Metarhizium , have also shown great potential for the management of planthoppers . Thus, genetically modified fungi might be enhanced in their virulence to sucking insects via the induction of insect RNAi .…”

Conidial Density and Viability of Beauveria bassiana Isolates from Java and Sumatra and Their Virulence Against Nilaparvata lugens at Different Temperatures

“…The effort to control population of N. lugens had been done through synthetic chemical control (Baehaki & Suparno, 2018;Liu et al, 2013;Zhang et al, 2014) and biological control by using entomopathogenic fungi, such as Beauveria bassiana (Lee et al, 2015;Li, Lin, Li, Xu, & Feng, 2012) and Metarhizium anisopliae (Chinniah, Ravikumar, Kalyanasundaram, & Parthiban, 2016) have been proven to be effective agents to control the BPH. B. bassiana could kill BPH more than 80% (Lee et al, 2015;Li et al, 2014) and to kill the eggs…”