Cows which represent top of the genetic value of the population in regard to their traits are chosen for bull dams. Selection is based on origin, production performance (quantity of milk, milk fat and protein content), milking/dairy traits, reproductive indicators, exterior and linear type scores. This study included 292 cows of Simmental breed selected for bull dams on the territory of Republic of Serbia during one year. Selection of cows for herd of bull dams was done after finalized first and subsequent lactation. In this study, the effect of region and year of calving on milk traits is presented. Applying the method of least squares average value of milk production was obtained-5.754,49 kg, milk fat content-3,98% and quantity of milk fat-230,24 kg. Regions had statistically highly significant effect (P<0,01) on all investigated milk traits. Year of calving showed no statistically significant effect on milk yield, yield of milk fat and content of milk fat (P>0,05).