Selection of DNA aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis

Xu, Shengmin; Yuan, Hang; Chen, Shaopeng; Xu, An; Wang, Jun; Wu, Lijun

doi:10.1016/j.ab.2012.01.026

Cited by 73 publications

(51 citation statements)

References 46 publications

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“…SELEX has been successfully applied to a variety of targets including proteins [44], small peptides [45], metal ions [46], and small organic molecules [47]. In basic medical research, this method has been used for drug screening [48][49][50][51], clinical diagnosis [52], and treatment [53].…”

Section: Discussionmentioning

confidence: 99%

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Cui¹,

Liu²,

Su³

et al. 2016

ABBS

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Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is considered to be a major etiological factor for dental caries. In this study, plaques from dental enamel surfaces of caries-active and caries-free individuals were obtained and cultivated for S. mutans isolation. Morphology examination, biochemical characterization, and polymerase chain reaction were performed to identify S. mutans. The cariogenicity of S. mutans strains isolated from clinical specimens was evaluated by testing the acidogenicity, aciduricity, extracellular polysaccharide production, and adhesion ability of the bacteria. Finally, subtractive SELEX (systematic evolution of ligands by exponential enrichment) technology targeting whole intact cells was used to screen for ssDNA aptamers specific to the strains with high cariogenicity. After nine rounds of subtractive SELEX, sufficient pool enrichment was achieved as shown by radioactive isotope analysis. The enriched pool was cloned and sequenced randomly, followed by MEME online and RNA structure software analysis of the sequences. Results from the flow cytometry indicated that aptamers H1, H16, H4, L1, L10, and H19 could discriminate highly cariogenic S. mutans strains from poorly cariogenic strains. Among these, Aptamer H19 had the strongest binding capacity with cariogenic S. mutans strains with a dissociation constant of 69.45 ± 38.53 nM. In conclusion, ssDNA aptamers specific to highly cariogenic clinical S. mutans strains were successfully obtained. These ssDNA aptamers might be used for the early diagnosis and treatment of dental caries.

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Section: Discussionmentioning

confidence: 99%

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Cui¹,

Liu²,

Su³

et al. 2016

ABBS

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“…These interactions all have been successfully applied into promoting the selectivity of SERS-based method into specific target monitoring [28][29][30][31]. Host-guest interaction is an important phenomenon in supramolecular chemistry, which describes the special interaction between the host molecules with unique structure and the guest smaller molecules or ions.…”

Section: Host-guest Interactionmentioning

confidence: 99%

“…Beside the common antibody modification, other interactions that base on immunological recognition has also been applied into the detection of specific pollutants recently. For example, aptamer is made of single-stranded DNA oligomer that can be selected against specific target (biological macromolecule or small organic molecule) come from systematic evolution of ligands by exponential enrichment (SELEX) [31]. On the basis of capture and enrichment by the PCB-77 specific aptamer, the improved detection of PCB77 was accomplished with a LOD down to 1 × 10 −8 mol L −1 [52].…”

Section: Antibody-antigen Interactionmentioning

confidence: 99%

Precision Target Guide Strategy for Applying SERS into Environmental Monitoring

Ouyang¹,

Li²,

Zhu³

et al. 2017

Raman Spectroscopy and Applications

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Surface enhanced Raman spectroscopy (SERS) is a promising analytical technique that exhibits various applications in trace detection and identification. When it is applied into environmental monitoring, we should concern several key points to improve detection sensitivity and selectivity for the detection in complex matrix. In this tutorial review, we mainly focus on the strategies for improving the use of SERS into environmental application. The strategies are summarized for enhancing the ability of the substrate to selectively capture specific targets, and for achieving separation and concentration of the analytes from the matrix and the assembly structures for multiple phase detection. We have also introduced several newly developed detection systems using portable instruments and miniaturized devices that are more suitable for infield applications. In addition, we discuss the present challenges that hide it from wide real application and give the outlook for the future development in applying SERS in environmental monitoring.

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“…As a new platform technology, aptamer-based techniques bioassay has recently been adopted in food analysis, clinical, medical and environmental monitoring [80][81][82][83][84][85]. In food safety monitoring, aptamers are applied for detection of chemical and/or biological hazards.…”

Section: Application Of Aptamer In Food Safetymentioning

confidence: 99%

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Ndikubwimana¹

2015

J Bioprocess Biotech

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Selection of DNA aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis

Cited by 73 publications

References 46 publications

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Precision Target Guide Strategy for Applying SERS into Environmental Monitoring

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

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Selection of DNA aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis

Cited by 73 publications

References 46 publications

Identification of ssDNA aptamers specific to clinical isolates of &lt;italic&gt;Streptococcus mutans&lt;/italic&gt; strains with different cariogenicity

Identification of ssDNA aptamers specific to clinical isolates of &lt;italic&gt;Streptococcus mutans&lt;/italic&gt; strains with different cariogenicity

Precision Target Guide Strategy for Applying SERS into Environmental Monitoring

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Contact Info

Product

Resources

About

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity