Selection of DNA Aptamers That Recognize α-Synuclein Oligomers Using a Competitive Screening Method

Tsukakoshi, Kaori; Abe, Koichi; Sode, Koji; Ikebukuro, Kazunori

doi:10.1021/ac300330g

Cited by 185 publications

(163 citation statements)

References 32 publications

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“…However,t he selection of aptamersf or Ab is difficult, and the target range of nucleic acid aptamers is restricted owing to their low pI. [73,74] Peptides, as an alternative to nucleic acid aptamers for protein binding,c an give ab road target range for protein detection because of their simple chemicals tructure, synthetic accessibility,a nd the greater functional diversity of amino acidsa sc ompared with nucleotides. Li's group reported that Ab 1-42 oligomers could be determined by protein-binding peptidesw ith electrochemical reporters via supermolecule formation ( Figure 8).…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

Detection of Aβ Monomers and Oligomers: Early Diagnosis of Alzheimer's Disease

Zhou

Liu

Hao

et al. 2016

Chemistry An Asian Journal

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Alzheimer's disease (AD), as the most common progressive neurodegenerative disorder, is pathologically characterized by deposition of extracellular plaque composed of amyloid-β peptide (Aβ). Different assembled states of Aβ have been considered as both important biomarkers and drug targets for the diagnosis and therapy of AD. Recent studies demonstrate that small, diffusible Aβ oligomers formed by aggregation of Aβ monomers are the major toxic agents in AD. Therefore, the development of reliable assays for Aβ (both monomers and oligomers) will be important for the early differential diagnosis of dementia, predicting the progression of AD, as well as monitoring the effectiveness of novel anti-Aβ drugs for AD. In this review, we summarize the recent progress made in the development of techniques for detection of Aβ monomers and oligomers. In particular, the principles governing the design of these sensors are classified and summarized. Moreover, the advantages and disadvantages of the assays are evaluated. This review also discusses the improvements and challenges for application of these assays in the early diagnosis of AD.

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Section: Electrochemical Sensorsmentioning

confidence: 99%

Detection of Aβ Monomers and Oligomers: Early Diagnosis of Alzheimer's Disease

Zhou

Liu

Hao

et al. 2016

Chemistry An Asian Journal

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“…Moreover, the reported antibody of Aβ oligomer also recognizes Aβ monomer and other Aβ aggregates and metabolites to some extent. 36 Alternatively, the organic dye-based fluorescence assays (eg, thioflavin T [ThT]) have been commonly used for monitoring the formation of Aβ aggregates in laboratory investigation. 37,38 However, most of the dyes cannot be used to discriminate Aβ oligomer from other β-sheets of Aβ aggregates, 37 thus limiting their applications for the routine test of Aβ oligomer for early diagnosis of AD.…”

Section: Introductionmentioning

confidence: 99%

A sensitive and selective electrochemical biosensor for the determination of beta-amyloid oligomer by inhibiting the peptide-triggered in situ assembly of silver nanoparticles

Xing¹,

Feng²,

Zhang³

et al. 2017

IJN

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Soluble beta-amyloid (Aβ) oligomer is believed to be the most important toxic species in the brain of Alzheimer’s disease (AD) patients. Thus, it is critical to develop a simple method for the selective detection of Aβ oligomer with low cost and high sensitivity. In this paper, we report an electrochemical method for the detection of Aβ oligomer with a peptide as the bioreceptor and silver nanoparticle (AgNP) aggregates as the redox reporters. This strategy is based on the conversion of AgNP-based colorimetric assay into electrochemical analysis. Specifically, the peptide immobilized on the electrode surface and presented in solution triggered together the in situ formation of AgNP aggregates, which produced a well-defined electrochemical signal. However, the specific binding of Aβ oligomer to the immobilized peptide prevented the in situ assembly of AgNPs. As a result, a poor electrochemical signal was observed. The detection limit of the method was found to be 6 pM. Furthermore, the amenability of this method for the analysis of Aβ oligomer in serum and artificial cerebrospinal fluid (aCSF) samples was demonstrated.

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“…We have used an aptamer previously reported to recognise oligomers and fibrils formed from αS and Aβ21 to enable the sensitive and specific visualisation of protein aggregates at the nanoscale. Aptamers are single‐stranded oligonucleotides developed to have high affinity and specificity and can be made for almost any molecule or structure 22, 23.…”

mentioning

confidence: 99%

Nanoscopic Characterisation of Individual Endogenous Protein Aggregates in Human Neuronal Cells

et al. 2018

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The aberrant misfolding and subsequent conversion of monomeric protein into amyloid aggregates characterises many neurodegenerative disorders, including Parkinson's and Alzheimer's diseases. These aggregates are highly heterogeneous in structure, generally of low abundance and typically smaller than the diffraction limit of light (≈250 nm). To overcome the challenges these characteristics pose to the study of endogenous aggregates formed in cells, we have developed a method to characterise them at the nanometre scale without the need for a conjugated fluorophore. Using a combination of DNA PAINT and an amyloid‐specific aptamer, we demonstrate that this technique is able to detect and super‐resolve a range of aggregated species, including those formed by α‐synuclein and amyloid‐β. Additionally, this method enables endogenous protein aggregates within cells to be characterised. We found that neuronal cells derived from patients with Parkinson's disease contain a larger number of protein aggregates than those from healthy controls.

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Selection of DNA Aptamers That Recognize α-Synuclein Oligomers Using a Competitive Screening Method

Cited by 185 publications

References 32 publications

Detection of Aβ Monomers and Oligomers: Early Diagnosis of Alzheimer's Disease

Detection of Aβ Monomers and Oligomers: Early Diagnosis of Alzheimer's Disease

A sensitive and selective electrochemical biosensor for the determination of beta-amyloid oligomer by inhibiting the peptide-triggered in situ assembly of silver nanoparticles

Nanoscopic Characterisation of Individual Endogenous Protein Aggregates in Human Neuronal Cells

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