Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.

Abstract

Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred.Electronic supplementary materialThe online version of this article (10.1007/s10142-018-0597-9) contains supplementary material, which is available to authorized users.

FishSNP: a high quality cross-species SNP database of fishes

“…The presence of homologous genes or sequences initially complicates allele identification, however the simplicity with which SNPs can be scored promotes allele discrimination 6 . SNP markers have been widely employed in fish disease resistance 7 , 8 , feed conversion efficiency 9 , 10 , growth rate 11 , 12 , muscle yield 13 , reproductive characteristics 14 , 15 and tolerance to environmental stressors 16 , 17 since the emergence of high-throughput sequencing technology. Similarly, SNP marker-related sequencing data increased rapidly.…”

Biotech in China 2021, at the beginning of the 14th five-year period (“145”)

“…Examples are transgenic Yellow River carps (Luo et al 2018 ), engineered at the CAS Institute of Hydrobiology for a triploid expression of the fish’s growth hormone leading to a nearly twofold faster growth, and the goat Yangang, created by somatic cloning at Northwest A&F University (Zhi 2016 ).…”

Elite allele mining for growth rate traits in common carp ( Cyprinus carpio ) by association analysis

“…Currently, association mapping has been used extensively in the mining of excellent alleles of economic traits in livestocks (Brym et al., 2011; Justyna et al., 2017; Nalaila et al., 2012; Podisi et al., 2011) and crops (Dang et al., 2015; Wen et al., 2008; Yang et al., 2011). However, only a few studies were reported in aquaculture animals (Jiang et al., 2019; Li et al., 2020; Luo et al., 2018; Zheng et al., 2016).…”