The objective of this research was to examine the population structure and production of Madura cows. Indonesian local Madura cows are originally from Madura island. Its development is constantly improved to sustain the existence and production of Madura cows as the main contributor to beef. The research was conducted in Lenteng sub-district, Sumenep district, East Java province from August to September 2023. The research method used was the survey method and sampling was done by accidental sampling. Madura cows data used were 263 heads. Data analysis using descriptive. The results showed that the population structure of Madura cows was 9.89% male calves and 13.69% females, 7.22% young males and 28.52% females, 0% bull and 41% cows. The number of cows to the population was 40.68%, the number of births to the population was 13.69%, the number of mortalities to the population was 2.28%, the Natural Increase was 11.41% with Calf Crop was 29.51%. The results indicated that the production of Madura cows is still low. It is suggested to procure selected males for artificial insemination or natural mating. Based on the population structure there is a rarity of males.