Selection of immature bovine oocytes for developmental potential in vitro

Madison, V.; Avery, B.; Grève, T.

doi:10.1016/0378-4320(92)90065-l

Cited by 89 publications

(43 citation statements)

References 18 publications

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“…Since, only grade A and B oocytes were used in this study, that oocytes from early atretic follicles showed a higher, while those from late atretic follicles a lower developmental potential suggested a high correlation between COCs morphology and oocyte competence. Studies on the bovine have also shown that COCs with good morphology tended to develop better in vitro, although some indicated that oocytes with incomplete cumulus developed as well (Madison et al 1992, Blondin & Sirard 1995, de Wit et al 2000. However, both this and other studies showed that the COCs with good morphology could have come from the late atretic follicles and developed poorly (Jewgenow et al 1999, de Wit et al 2000.…”

Section: Discussionmentioning

confidence: 74%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

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Interactive effects of granulosa cell apoptosis, follicle size, cumulus-oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system Zheng-Bin Han, Guo-Cheng Lan, Yan-Guang Wu, Dong Han, Wei-Guo Feng, Jun-Zuo Wang and Jing-He Tan AbstractUsing a well-in-drop (WID) oocyte/embryo culture system that allows identification of follicular origin, we have investigated the effects of granulosa cells (GCs) apoptosis, follicle size, cumulus-oocyte complexes (COCs) morphology, and cumulus expansion on the developmental competence of goat oocytes matured and cultured individually following parthenogenetic activation. The WID system supported oocyte maturation and embryo development to a level similar to the conventional group system. The majority of goat oocytes acquired competence for development up to the 8-16 cell stage in follicles larger than 2 mm, but did not gain the ability to form morula/blastocyst (M/Bs) until follicles larger than 3 mm in diameter. The extent of atresia affected M/Bs formation. This effect varied according to the follicle size. Cumulus expansion increased with follicle size and decreased with increasing incidence of GCs apoptosis. Oocyte developmental potential was also correlated with cumulus expansion. Regardless of the degree of follicle atresia, 73-84% of the floating cells in the follicular fluid (FF) underwent apoptosis. Correlation between floating cell density in FF and oocyte developmental potency suggests the possibility to use the floating cell density as a simple and non-invasive marker for oocyte quality. It is concluded that the developmental potential of an oocyte is determined by multifactor interactions, and multiple factors must be considered together to accurately predict the quality of an oocyte. Reproduction (2006) 132 749-758Introduction A number of studies have shown that in vitro development of pre-implantation stage mammalian embryos is better when cultured in groups (group culture) than cultured singly in microdrops (microdrop individual culture, MIC) (Gardner et al. 1994, Kato & Tsunoda 1994, Keefer et al. 1994, Moessner & Dodson 1995. However, apart from the large-scale production of embryos for experimental purposes, most commercial and human embryo culture systems require culturing individually or in small groups. The reason for this need is the small number of oocytes available (ovum pick-up (OPU) technology, single slaughtered valuable oocyte donors and human IVF) or the required identification after culture. Besides, sometimes zona-free eggs must be cultured in isolation from each other to prevent conglomeration. Therefore, the establishment of an individual culture system that is as efficient as group culture has become of prime importance. Furthermore, although there are quite a few reports on individual culture of embryos, studies on individual culture of oocytes during maturation are few and with conflicting results (Carolan et al. 1996, Hagemann et al. 1998, Jewgenow et al. 19...

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Section: Discussionmentioning

confidence: 74%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

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“…There is considerable morphological variability among oocytes capable of normal development, and assessment of morphology has led to some improvements in the identification of oocytes that would develop in vitro (De Loose et al 1992). Although morphology can indicate maturity and fertilisation potential (Madison et al 1992), even after careful selection on the basis of visual appearance, 10 -20% of harvested immature oocytes from slaughterhouse pig ovaries degenerate during sperm co-incubation (Matas et al 1996). This suggests that other approaches warrant investigation.…”

Section: Discussionmentioning

confidence: 99%

Mitochondria directly influence fertilisation outcome in the pig

Shourbagy¹,

Spikings²,

Freitas³

et al. 2006

Reproduction

296

222

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The mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome. In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB). Using porcine in vitro fertilisation (IVF), we have assessed oocyte maturation, cytoplasmic volume, fertilisation outcome, mitochondrial number as determined by mtDNA copy number, and whether mitochondria are uniformly distributed between blastomeres of each embryo. After staining with BCB, we observed a significant difference in cytoplasmic volume between BCB positive (BCB 1 ) and BCB negative (BCB 2 ) oocytes. There was also a significant difference in mtDNA copy number between fertilised and unfertilised oocytes and unequal mitochondrial segregation between blastomeres during early cleavage stages. Furthermore, we have supplemented BCB 2 oocytes with mitochondria from maternal relatives and observed a significant difference in fertilisation outcomes following both IVF and intracytoplasmic sperm injection (ICSI) between supplemented, sham-injected and non-treated BCB 2 oocytes. We have therefore demonstrated a relationship between oocyte maturity, cytoplasmic volume, and fertilisation outcome and mitochondrial content. These data suggest that mitochondrial number is important for fertilisation outcome and embryonic development. Furthermore, a mitochondrial pre-fertilisation threshold may ensure that, as mitochondria are diluted out during post-fertilisation cleavage, there are sufficient copies of mtDNA per blastomere to allow transmission of mtDNA to each cell of the post-implantation embryo after the initiation of mtDNA replication during the early postimplantation stages.Reproduction (

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“…After aspiration, the syringe contents were slowly dispelled into sterile Petri dish (60 mm) for searching oocytes under a stereo-microscope. The oocytes collected were washed three times in PBS, evaluated under an inverted microscope and classified into compact oocytes with ≥5 layers of complete cumulus cells and evenly granulated dark ooplasm (COCs), partial denuded, denuded, shrunken and fragment oocytes (Madison et al 1992). Only, COCs were used for in vitro maturation.…”

Section: Oocyte Collectionmentioning

confidence: 99%

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Abdel-Khalek¹,

Gabr²,

Khalil³

et al. 2017

Polish Journal of Veterinary Sciences

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Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in a blastocyst stage. This study represented a successful in vitro production of Sudanese dromedary camel embryos from epididymal sperm cells and in vitro matured oocytes recovered from slaughtered camels.

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Selection of immature bovine oocytes for developmental potential in vitro

Cited by 89 publications

References 18 publications

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Mitochondria directly influence fertilisation outcome in the pig

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

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