Selection of lactic acid bacteria as candidate probiotics and in vivo test on Artemia nauplii

Abstract

Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La. casei BR51 and X2 were preferred, as they were efficient even in the absence of nutrient supply. La. casei X2 was finally selected as candidate probiotic, due to the best growth performances of Artemia, with or without the pathogen.

Dietary supplementation of probiotics improves survival and growth of Rohu (Labeo rohita Ham.) hatchlings and fry in outdoor tanks

“…Kumar et al (2013) reported that seaweed powder plays an important role in improving overall health status and innate immunity in Rohu. Lamari et al (2014) reported that several LAB strains seem to exert a beneficial role in the structure of fish. Cao et al (2007) and Baruah et al (2005) also have reported that incorporation of microbial phytase in basal diets improved overall growth performance and bioavailability of nutrients in juvenile Rohu.…”

Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects

“…Some authors reported 100% Artemia mortality after 24 to 48 h of V. alginolyticus administration (Rico-Mora & Voltolina 1995, Mahdhi et al 2011, Lamari et al 2014, while other studies revealed no Artemia mortality in the same period (Gomez-Gil et al 1998). In this study, V. alginolyticus 03/8525 significantly reduced Artemia survival after 48 and 72 h of the administration, whereas the preventive colonization of Artemia with Lactobacillus pentosus H16 protects the nauplii against mortality.…”

CINÉTICA, PRUEBA DE CRECIMIENTO Y EFECTO DE INHIBICIÓN DELactococcus lactisSOBREYersinia pseudotuberculosis

“…los probióticos tienen un efecto benéfico en el tracto intestinal, manteniendo y fortaleciendo los mecanismos de defensa, sin alterar las funciones fisiológicas y bioquímicas [2]. los principales géneros utilizados como probióticos en cultivos son Aspergillus, Saccharomyces, Bacillus, Bacteroides y Lactobacillus [3]. la yersinia es un microorganismo infeccioso importante en humanos y animales.…”