Selection of Microorganisms Producing L-Serine from Glycine

Abstract

Various wild and mutant strains of bacteria, yeasts, actinomycetes and fungi (total 1416 strains) were cultured in a medium containing DL-glyceric acid (10 mg/mi), L-threo nine (10 mg/ml) or glycine (5 mg/ml).As a result, Nocardia butanica ATCC 21197, Nocardia paraffinica ATCC 21198 and Brevibacterium ketoglutamicum ATCC 15587 were found to accumulate 1 mg/ml of L-serine in the broth. These 3 strains were belonged to a group of microorganisms which were weak in L-serine decomposition activity, although most microorganisms decomposed over 90% of the L-serine into some compounds other than glycine.Some microorganisms produced a-aminobutyrate, L-isoleucine or glycine from L-threonine.