The aim of the study was to group the lactation curve (LC) of Holstein cows in several clusters based on their milking characteristics and to investigate physiological differences among the clusters. Milking data of 330 lactations which have a milk yield per day during entire lactation period were used. The data were obtained by refinement from 1332 lactations from 724 cows collected from commercial farms. Based on the similarity measures, clustering was performed using the k-medoids algorithm; the number of clusters was determined to be six, following the elbow method. Significant differences on parity, peak milk yield, DIM at peak milk yield, and average and total milk yield (p < 0.01) were observed among the clusters. Four clusters, which include 82% of data, show typical LC patterns. The other two clusters represent atypical patterns. Comparing to the LCs generated from the previous models, Wood, Wilmink and Dijsktra, it is observed that the prediction errors in the atypical patterns of the two clusters are much larger than those of the other four cases of typical patterns. The presented model can be used as a tool to refine characterization on the typical LC patterns, excluding atypical patterns as exceptional cases.