Selection of polyvalent bacteriophages infecting Salmonella enterica serovar Choleraesuis

Parra, Bárbara; Robeson, James

doi:10.1016/j.ejbt.2016.01.008

Cited by 18 publications

(31 citation statements)

References 15 publications

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“…The genome of the bacteriophage FP01 was obtained in a single contig of 44,900 bp ( Fig. 1), coincident with the previous description of the gDNA molecular weight of the phage FP01 [18]. The genome of the phage FP01 has a total of 90 CDSs with a 49.41% G+C content ( Fig.…”

Section: Resultssupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

“…The polyvalent bacteriophage FP01 was isolated from wastewater in the V region of Chile, using Salmonella enterica serotype Choleraesuis VAL201 as host [18]. The phage FP01 belongs to the order Caudoviridae, family Siphoviridae, which are bacterial viruses of double-strand DNA [18]. The bacteriophage FP01 is able to proliferate in E. coli C, E. coli B, E. coli K12, and Salmonella enterica serovars Typhi, Paratyphi B, and Choleraesuis [18], indicating that FP01 has a common attachment site on the susceptible bacterial species [18,19].…”

Section: Introductionmentioning

confidence: 99%

“…The phage FP01 belongs to the order Caudoviridae, family Siphoviridae, which are bacterial viruses of double-strand DNA [18]. The bacteriophage FP01 is able to proliferate in E. coli C, E. coli B, E. coli K12, and Salmonella enterica serovars Typhi, Paratyphi B, and Choleraesuis [18], indicating that FP01 has a common attachment site on the susceptible bacterial species [18,19]. A better understanding of the mechanisms and evolution of polyvalent bacteriophages can be gained by comparative genomic analysis.…”

Section: Introductionmentioning

confidence: 99%

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Genomic Analysis of the Polyvalent Bacteriophage FP01

Vásquez¹,

Retamales²,

Parra³

et al. 2019

Preprint

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Recently the polyvalent bacteriophage FP01, isolated from wastewater in Valparaiso, Chile, was described to have lytic activity across species against Escherichia coli and Salmonella enterica serovars. Due to it polyvalent nature the bacteriophage FP01 could have potential application in food and agri-industry. Also, fundamental aspects of polyvalent bacteriophage biology are not well known. In this study we sequenced and describe the complete genome of the polyvalent phage FP01 (MH745368) using the nanopore technology. The bacteriophage FP01 genome has a 44,900 bp, double-stranded DNA with an average G+C content of 49.41% and 90 coding sequences (CDSs). We found that the phage FP01 critically depends on host factors for replication and transcription. Also, it has a critical lysogenic repressor pseudogene. Phylogenetic analyses indicated that the phage FP01 is closely related to phages lambda and P22. These results suggest that the phage FP01 could be a lytic variant of a lysogenic phage or acquired genes from lysogenic phages during host infection.

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Section: Resultssupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic Analysis of the Polyvalent Bacteriophage FP01

Vásquez¹,

Retamales²,

Parra³

et al. 2019

Preprint

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“…Phages are usually species-specific and even strain-specific8, however, some polyvalence is observed, predominantly among phages of Enterobacteria910 and staphylococci11. These polyvalent phages are able to infect strains from either different genera or species12.…”

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Characterization of two polyvalent phages infecting Enterobacteriaceae

Hamdi

Rousseau²,

Labrie³

et al. 2017

Sci Rep

115

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Bacteriophages display remarkable genetic diversity and host specificity. In this study, we explore phages infecting bacterial strains of the Enterobacteriaceae family because of their ability to infect related but distinct hosts. We isolated and characterized two novel virulent phages, SH6 and SH7, using a strain of Shigella flexneri as host bacterium. Morphological and genomic analyses revealed that phage SH6 belongs to the T1virus genus of the Siphoviridae family. Conversely, phage SH7 was classified in the T4virus genus of the Myoviridae family. Phage SH6 had a short latent period of 16 min and a burst size of 103 ± 16 PFU/infected cell while the phage SH7 latent period was 23 min with a much lower burst size of 26 ± 5 PFU/infected cell. Moreover, phage SH6 was sensitive to acidic conditions (pH < 5) while phage SH7 was stable from pH 3 to 11 for 1 hour. Of the 35 bacterial strains tested, SH6 infected its S. flexneri host strain and 8 strains of E. coli. Phage SH7 lysed additionally strains of E. coli O157:H7, Salmonella Paratyphi, and Shigella dysenteriae. The broader host ranges of these two phages as well as their microbiological properties suggest that they may be useful for controlling bacterial populations.

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