Selection of potential probiotic bifidobacteria and prebiotics for elderly by using in vitro faecal batch cultures

Valdés, Lorena; Salazar, Nuria; González, Sonia; Arboleya, Silvia; Ríos-Covián, David; Genovés, Salvador; Ramón, Daniel; Reyes‐Gavilán, Clara G. de los; Ruas‐Madiedo, Patricia; Gueimonde, Miguel

doi:10.1007/s00217-016-2732-y

Cited by 22 publications

(18 citation statements)

References 36 publications

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“…Thus far, the study of the internalization of prebiotics into prokaryotes is still in an early stage, although a large amount of research has been focused on how foreign nanoparticles are internalized into eukaryotic cells through endocytosis 11 . Most research on prebiotic materials have mainly focused on fermentation by probiotics 12 , 13 . By contrast, one of the goals of our research is to develop prebiotics in nanoparticle form and to elucidate their internalization into probiotics.…”

Section: Discussionmentioning

confidence: 99%

A new way of producing pediocin in Pediococcus acidilactici through intracellular stimulation by internalized inulin nanoparticles

Kim

Lee

Singh

et al. 2018

Sci Rep

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One of the most challenging aspects of probiotics as a replacement for antibiotics is to enhance their antimicrobial activity against pathogens. Given that prebiotics stimulate the growth and/or activity of probiotics, we developed phthalyl inulin nanoparticles (PINs) as prebiotics and observed their effects on the cellular and antimicrobial activities of Pediococcus acidilactici (PA). First, we assessed the internalization of PINs into PA. The internalization of PINs was largely regulated by glucose transporters in PA, and the process was energy-dependent. Once internalized, PINs induced PA to produce substantial amounts of antimicrobial peptide (pediocin), which is effective against both Gram-positive (Salmonella Gallinarum) and Gram-negative (Listeria monocytogenes) pathogens. When treated with small-sized PINs, PA witnessed a nine-fold increase in antimicrobial activity. The rise in pediocin activity in PA treated with PINs was accompanied by enhanced expression of stress response genes (groEL, groES, dnaK) and pediocin biosynthesis genes (pedA, pedD). Although the mechanism is not clear, it appears that the internalization of PINs by PA causes mild stress to activate the PA defense system, leading to increased production of pediocin. Overall, we identified a prebiotic in nanoparticle form for intracellular stimulation of probiotics, demonstrating a new avenue for the biological production of antimicrobial peptides.

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Section: Discussionmentioning

confidence: 99%

A new way of producing pediocin in Pediococcus acidilactici through intracellular stimulation by internalized inulin nanoparticles

Kim

Lee

Singh

et al. 2018

Sci Rep

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“…Previously described primers and conditions [20] were used for quantification of the different bacterial populations analyzed ( Akkermansia , Bacteroides group, Bifidobacterium , Clostridium cluster XIVa, Lactobacillus group, and Faecalibacterium ). qPCR reactions were performed in a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using SYBR Green chemistry (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Age-Associated Changes in Gut Microbiota and Dietary Components Related with the Immune System in Adulthood and Old Age: A Cross-Sectional Study

et al. 2019

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The fecal microbiota plays an important role in human health, and alterations in the microbiota–host interaction seem to be involved in the ageing process. Therefore, it is of interest to develop strategies for promoting a balanced microbiota in old age in order to prevent the physiological and immune decline associated with age. However, the specific microbiota changes in the transition from adulthood to senescence are not yet well understood. Here we assessed the levels of some intestinal microorganisms and short chain fatty acids (SCFAs) across different age-groups. In total, 153 adults from four age groups (<50, 50–65, 66–80, and >80 years-old) were recruited; the levels of different bacterial groups in fecal samples were determined by quantitative polymerase chain reaction (qPCR), and those of SCFA by gas chromatography. Dietary information was collected by using a Food Frequency Questionnaire. The presence of the Bifidobacterium, Faecalibacterium, Bacteroides group, and Clostridium cluster XIVa decreased with age up to 66–80 years of age, with differences reaching statistical significance for the latter group. Interestingly, the levels of some of these microorganisms recovered in the very old age group (>80 years), with these older individuals presenting significantly higher counts of Akkermansia and Lactobacillus group than adults and the younger elderly. In addition, ageing was associated with a progressively and statistically significant reduction in the fecal concentrations of SCFAs. Dietary intakes also showed some statistically significant differences among the groups for some macro- and micronutrients. Moreover, associations of some microorganisms with age and macronutrients were also evidenced. Considering the role that fecal microbiota alterations may have in terms of impairing homeostasis and resilience, our results underline the importance of understanding the ageing and immunosenescence processes by including the microbiota perspective.

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“…The levels of Bacteroides-Prevotella-Porphyromonas group, Faecalibacterium, Bifidobacterium, Lactobacillus-group, Staphylococcus, Akkermansia, Enterobacteria, Clostridium XIVa group, as well as of total bacteria, were determined by quantitative PCR (qPCR) using previously described primers and conditions 38 , 39 .…”

Section: Methodsmentioning

confidence: 99%

Fecal microbiota profile in a group of myasthenia gravis patients

Morís

Arboleya

Mancabelli

et al. 2018

Sci Rep

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The intestinal microbiota plays a key role in the maintenance of human health. Alterations in this microbiota have been described in several autoimmune diseases, including nervous system diseases. Nevertheless, the information regarding neuromuscular conditions is still limited. In this study, we aimed at characterizing the intestinal microbiota composition in myasthenia gravis patients (MG). To this end fecal samples were taken from ten patients, with antibodies against the acetylcholine receptor, and ten age and sex matched controls from the same population (Asturias region, Spain). Fecal samples were submitted to microbiota analyses by 16S rRNA gene profiling, bifidobacterial ITS-region profiling and qPCR. The fecal levels of short chain fatty acids were determined by gas chromatography. MG patients were found to harbor lower relative proportions of Verrucomicrobiaceae and Bifidobacteriaceae, among others, and increased of the phylum Bacteroidetes and the family Desulfovibrionaceae. The increase of these latter microbial groups was also confirmed at quantitative level by qPCR. In contrast, no statistically significant differences were found between MG patients and the control group in the bifidobacterial population at the species level or in short chain fatty acids profiles. Our data indicates an altered fecal microbiota pattern in MG patients and point out at specific microbiota targets for intervention in this population.

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Selection of potential probiotic bifidobacteria and prebiotics for elderly by using in vitro faecal batch cultures

Abstract: potentially probiotic strains and prebiotic substrates for the development of functional foods specifically targeted to the senior population.

Cited by 22 publications

References 36 publications

A new way of producing pediocin in Pediococcus acidilactici through intracellular stimulation by internalized inulin nanoparticles

A new way of producing pediocin in Pediococcus acidilactici through intracellular stimulation by internalized inulin nanoparticles

Age-Associated Changes in Gut Microbiota and Dietary Components Related with the Immune System in Adulthood and Old Age: A Cross-Sectional Study

Fecal microbiota profile in a group of myasthenia gravis patients

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