Selection of prepubertal goat oocytes using the brilliant cresyl blue test

Rodrı́guez-González, E.; López‐Béjar, Manel; Velilla, Esther; Paramio, M. T.

doi:10.1016/s0093-691x(02)00645-3

Cited by 99 publications

(79 citation statements)

References 32 publications

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“…We suggest that in this study, the concentration of cysteamine causes an increase in GSH intracytoplasmic concentration to aid the sperm head decondensation but not enough to increase the blastocyst formation. In our previous study [4] using BCB+ selected oocytes matured in the same culture medium but without cysteamine, the percentage of oocytes reaching the morulae plus the blastocyst stage was 12% compared with the 23.8% reached in this study when adding cysteamine to the maturation medium.…”

Section: Discussioncontrasting

confidence: 59%

“…Comparing oocytes exposed to the BCB test, BCB+ oocytes showed higher percentages of oocytes reaching MII, oocytes with 2 PN, oocytes with MPN formation, cleaved embryos, embryos developing beyond the 8-cell stage and the number of blastocysts cells than the BCBoocytes. Previous studies in our laboratory [4] have shown that prepubertal goat oocytes stained with BCB show significantly higher rates of nuclear maturation and normal fertilisation (2PN oocytes) than unstained oocytes (BCB-), although the percentage of blastocysts is still low (4%). Pujol et al [3] in calf oocytes have also shown increases in the percentages of the blastocyst yield of BCB+ compared to BCB-oocytes (12.3 vs. 1.6% respectively).…”

Section: Discussionmentioning

confidence: 82%

“…We suggest that cysteamine had a positive effect on embryo yield from prepubertal goats only when the BCB+ oocytes were used. Rodríguez-González et al [4] observed that BCB+ oocytes were larger than BCB-oocytes. This could mean that complete oocyte intraovarian growth is needed before cysteamine 184 E. Rodríguez-González et al could have a positive effect on oocyte maturation.…”

Section: Discussionmentioning

confidence: 97%

“…The BCB test has been used successfully to select oocytes for IVM-IVF in pigs [1,2] and cows [3]. In our earlier study with prepubertal goat oocytes matured without cysteamine [4], we showed that oocytes exposed and stained with BCB are larger (136.6 vs. 125.5 µm in diameter) and more able to develop to morula and blastocysts than unstained oocytes (12.0 vs. 3.6%). However, blastocyst development rates were still lower (4% in BCB+ oocytes) than those obtained with oocytes from adult goats [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 84%

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Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

et al. 2003

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-The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 µM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB-(unstained oocytes). The oocytes were matured in a conventional IVM medium supplemented with cysteamine 100 µM. The control group consisted of oocytes not exposed to BCB and matured without cysteamine. The IVM-oocytes were inseminated and cultured in synthetic oviductal fluid (SOF) for 7 days. The normal fertilisation rate (oocytes showing 2 pronuclei and 1 sperm tail) of BCB+ oocytes (40%) was higher than those of BCB-(21%) and control oocytes (22%). The percentage of morulae plus blastocysts was higher (P < 0.05) in the BCB+ group than in the BCB-group (23.8 vs. 5.1%, respectively). In conclusion, the integration of the BCB test and the addition of cysteamine in the protocol of in vitro embryo production from prepubertal goat oocytes has improved the developmental rates of embryo development. embryo / IVF / IVM / thiol

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Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 84%

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Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

et al. 2003

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“…The stage of nuclear maturation was assessed by phase-contrast microscopy (magnification, ×40). The criteria used to determine oocyte maturation stage have been described elsewhere (Rodriguez-Gonzalez et al, 2002). Oocytes were scored as: germinal vesicle (GV) stage, when their chromatin, either filamentous or slightly condensed, was enclosed in a nuclear membrane; germinal vesicle breakdown (GVBD) stage, when there was no visible nuclear membrane and chromatin was condensed; metaphase I (MI) stage, when the chromatin was distributed at the equator of a large spindle; anaphase I stage, when homologous chromosomes could be seen to move away from each other and there was a visible spindle; telophase I stage, when homologous chromosomes were apart, and metaphase II (MII) stage, when the chromatin was located at the spindle and the first polar body extrusion was detected.…”

Section: Evaluation Of Nuclear Maturation Stagementioning

confidence: 99%

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Hammami

Izquierdo

Catalá

et al. 2014

Animal

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The present study was designed to evaluate the effect of activin-A during the in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on nuclear maturation, blastocyst yield and blastocyst quality of prepubertal goat oocytes. In Experiment 1, three groups of oocytes were used during the IVM of prepubertal goat oocytes to determine the optimal concentration of recombinant human activin-A added to the maturation medium. Cumulus-oocyte complexes were matured in an IVM medium containing 0, 10 and 100 ng/ml (groups A0, A10 and A100), fertilized and in vitro cultured using standard procedures. In Experiment 2, the addition of 10 ng/ml activin-A at IVM (A10A0), IVC (A0A10) or IVM + IVC (A10A10) was studied and compared with the control group (A0A0). Results of the first experiment demonstrated that the addition of activin-A yielded similar percentages of maturation (⩽71.0%) and blastocyst formation rates (⩽24.9%) than the control group (A0). Experiment 2 showed that exposure of prepubertal goat oocytes to an IVC medium containing 10 ng/ml activin-A (A0A10) significantly increased the rates of development to the blastocyst stage, as compared with the control group (A0A0) (19.5 ± 2.21% v. 13.1 ± 2.37%, respectively; P < 0.05). With regard to the blastocyst quality, total number of cells, inner cell mass (ICM) and trophectoderm of prepubertal goat embryos produced in the presence of activin-A did not differ significantly among experimental groups. In summary, these results indicate that supplementation of the IVC medium with activin-A enhances embryo development of prepubertal goat oocytes.

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Induced cumulus expansion of poor quality buffalo cumulus oocyte complexes by Interleukin‐1beta improves their developmental ability

Chaubey

Kumar

et al. 2018

J of Cellular Biochemistry

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The present study was conceived with the aim of modulating the cumulus expansion characteristics of poor quality (BCB-) buffalo oocyte complexes (COCs) in order to improve their fertilization outcomes. BCB- COCs were subjected to in vitro maturation (IVM) in presence of Interleukin-1 beta (IL-1β) along with BCB- (control) and good quality (BCB+) COCs. Results were assessed morphologically, by scanning electron microscopy (SEM) and by expression analysis of cumulus expansion related genes. Also, numbers of zona pellucida bound spermatozoa were counted and development rates of oocytes were monitored under different groups. Expression of versican isoforms and ADAMTS-1 was observed to be significantly different between cumulus cells of BCB+ and BCB- COCs. Upon IL-1β supplementation, ADAMTS-1 expression increased in BCB- COCs along with corresponding cumulus expansion rates. SEM analysis also revealed improved cumulus expansion in IL-1β supplemented BCB- COCs. HAS2 and TNFAIP-6 were significantly up-regulated after IL-1β supplementation while PTGS2 expression remained unaffected. Significantly more numbers of sperms crossed the cumulus barrier, especially in 100 ng/mL IL-1β supplemented COCs. Besides, cleavage and blastocyst development rates were also improved upon IL-1β addition. We concluded that IL-1β supplementation in IVM medium can improve cumulus expansion and development ability of poor quality buffalo oocytes.

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Selection of prepubertal goat oocytes using the brilliant cresyl blue test

Cited by 99 publications

References 32 publications

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Induced cumulus expansion of poor quality buffalo cumulus oocyte complexes by Interleukin‐1beta improves their developmental ability

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