Selection of probiotic bacteria for prevention of allergic diseases: immunomodulation of neonatal dendritic cells

Abstract

SummaryModification of intestinal microbiota early in life by administration of probiotic bacteria may be a potential approach to prevent allergic disease. To select probiotic bacteria for in vivo purposes, we investigated the capacity of probiotic bacteria to interact with neonatal dendritic cells (DC) and studied the ensuing T cell polarizing effect. Immature DC were generated from cord blood-derived monocytes and maturation was induced by maturation factors (

RETRACTED: Preventative and Therapeutic Role of Probiotics in Various Allergic and Autoimmune Disorders: An Up-to-Date Literature Review of Essential Experimental and Clinical Data

“…Bifidobacterium bifidum was found to be most potent polarizer in vitro-cultured dendritic cells to drive Th1-cell responses involving increased IFN-g producing T cells concomitant with reduction of IL-4-producing T-cells. 78 In addition, T-cells stimulated by Bifidobacterium bifidum matured dendritic cells as producers of more IL-10. 79 Moreover, Lactobacillus rhamnosus, member of another genus of probiotic bacteria, modulates dendritic cell function to induce a novel form of T-cell hyporesponsiveness.…”

“…Th2 cytokines and IgE production # Maassen et al 50 Lctbs casei IL-10 and Th1 cytokines production " Niers et al 52,78,111 Bfdbm bifidum/infantis; LL Th2 cytokines/IL-10 production …”

Probiotics and immunity

“…28,31 In addition, when DC maturation is induced by lipopolysaccharide (LPS) with or without added TNF-α and IL-1β, the mere presence of certain LAB strains during this maturation process affects the ability of DCs to polarize the cytokine production of CD4+ T cells. 37 Using a similar protocol, it can be shown that Lactobacillus rhamnosus GG (LGG) is able to induce T-cell hyporesponsiveness 38 and that Lactobacillus reuteri ASM20016 or Lactobacillus casei NIZO B255, but not Lactobacillus plantarum NIZO B253, are capable of priming DCs for the induction of regulatory T cells. 39 Similar fi ndings have been reported for Lactobacillus paracasei NCC2461 in mixed lymphocyte reactions using murine splenic CD4+ T cells and allogeneic splenocytes as accessory cells.…”

Probiotics and health: An evidence-based review

“…In fact: (1) probiotics modulate and stabilize the composition of the microbiota and, therefore, may have immunomodulatory effects; (2) some probiotics are able to inhibit the inflammatory response of the intestinal immune system through inhibition of NF-B activation or in combination with an anti-apoptotic action on intestinal epithelial cells [31,32]; (3) some probiotics are able to increase the activity of Natural Killer (NK) cells [33,34], which are first line of defense as they can perform cytotoxic activities independent from prior sensitization to antigens; (4) some probiotics increase the secretion of mucus [22]; (5) some probiotics have a direct immunomodulatory action: after being captured in the Peyer's patches, they can induce the secretion of cytokines and the expression of co-stimulatory molecules by antigen presenting cells (APC) [35]; (6) some strains of lactobacilli induce dendritic cells (DC) maturation [36]. DC can, through their particular cytostructure, pass through the layer of epithelial cells and capture antigens directly from the lumen.…”