Selection of putative colorectal cancer markers by applying PCA on the soluble proteome of tumors: NDK A as a promising candidate

Álvarez-Chaver, Paula; Rodríguez-Piñeiro, Ana M.; Rodrı́guez-Berrocal, Francisco Javier; García-Lorenzo, Andrés; Cadena, Marı́a Páez de la; Martínez-Zorzano, Vicenta S.

doi:10.1016/j.jprot.2011.02.031

Cited by 17 publications

(14 citation statements)

References 57 publications

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“…Heat shock 70 kDa protein 4, also known as ischemia‐responsive protein‐94 (Irp94), is a ligand for natural killer cell receptor protein‐2 on dendritic cells, which in turn plays a role in both innate and adaptive immunity [60]. DJ‐1 or PARK7 is known as a tumor biomarker in several kinds of cancer [61] and has been shown to be increased in CRC compared to normal mucosa [62].…”

Section: Discussionmentioning

confidence: 99%

Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model

et al. 2013

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Cancer stem cells (CSCs) represent a minor population of self-renewing cancer cells that fuel tumor growth. As CSCs are generally spared by conventional treatments, this population is likely to be responsible for relapses that are observed in most cancers. In this work, we analyzed the preventive efficiency of a CSC-based vaccine on the development of liver metastasis from colon cancer in a syngeneic rat model. We isolated a CSC-enriched population from the rat PROb colon carcinoma cell line on the basis of the expression of the aldehyde dehydrogenase-1 (ALDH1) marker. Comparative analysis of vaccines containing lysates of PROb or ALDH high cells by mass spectrometry identifies four proteins specifically expressed in the CSC subpopulation. The expression of two of them (heat shock protein 27-kDa and aldose reductase) is already known to be associated with treatment resistance and poor prognosis in colon cancer. Preventive intraperitoneal administration of vaccines was then performed before the intrahepatic injection of PROb cancer cells. While no significant difference in tumor occurrence was observed between control and PROb-vaccinated groups, 50% of the CSC-based vaccinated animals became resistant to tumor development. In addition, CSC-based vaccination induced a 99.5% reduction in tumor volume compared to the control group. To our knowledge, this study constitutes the first work analyzing the potential of a CSC-based vaccination to prevent liver metastasis development. Our data demonstrate that a CSCbased vaccine reduces efficiently both tumor volume and occurrence in a rat colon carcinoma syngeneic model.

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Section: Discussionmentioning

confidence: 99%

Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model

et al. 2013

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“…Two-dimensional gel electrophoresis remains one of the most common approaches for mining cancer biomarkers as well as for studying the complex biological events underlying colorectal tumorigenesis, regardless of its technical limitations [13,19,20]. Numerous studies have been conducted to identify protein biomarkers of CRC using either biofluids [4,16,21,22] or colorectal tissues [19,[23][24][25][26][27][28][29][30]. However, until now, proteins that are specifically regulated during the transition of colorectal adenoma to carcinoma have not been identified as diagnostic biomarkers of CRC.…”

Section: Introductionmentioning

confidence: 99%

Proteomic and cytokine plasma biomarkers for predicting progression from colorectal adenoma to carcinoma in human patients

et al. 2013

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In the present study, we screened proteomic and cytokine biomarkers between patients with adenomatous polyps and colorectal cancer (CRC) in order to improve our understanding of the molecular mechanisms behind turmorigenesis and tumor progression in CRC. To this end, we performed comparative proteomic analysis of plasma proteins using a combination of 2DE and MS as well as profiled differentially regulated cytokines and chemokines by multiplex bead analysis. Proteomic analysis identified 11 upregulated and 13 downregulated plasma proteins showing significantly different regulation patterns with diagnostic potential for predicting progression from adenoma to carcinoma. Some of these proteins have not previously been implicated in CRC, including upregulated leucine-rich α-2-glycoprotein, hemoglobin subunit β, Ig α-2 chain C region, and complement factor B as well as downregulated afamin, zinc-α-2-glycoprotein, vitronectin, and α-1-antichymotrypsin. In addition, plasma levels of three cytokines/chemokines, including interleukin-8, interferon gamma-induced protein 10, and tumor necrosis factor α, were remarkably elevated in patients with CRC compared to those with adenomatous polyps. Although further clinical validation is required, these proteins and cytokines can be established as novel biomarkers for CRC and/or its progression from colon adenoma.

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“…However, the measurement of serum NDKA levels in this group was conceived to verify the correct quantification of the molecule using the commercial ELISA assay, and to corroborate the difference in serum NDKA levels between healthy controls and CRC patients evidenced in our previous work by Western blot29. In the screening cohort, a small number of CRC patients were also found, corresponding to a prevalence of 0.78% which is expected in a familial-risk screening context (0.65–0.7%)5152.…”

Section: Discussionmentioning

confidence: 77%

Evaluation of serum nucleoside diphosphate kinase A for the detection of colorectal cancer

Otero-Estévez

Chiara

Barcia-Castro

et al. 2016

Sci Rep

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We previously described the over-expression of nucleoside diphosphate kinase A (NDKA) in tumours and serum from colorectal cancer (CRC) patients, suggesting its use as biomarker. In this study we evaluated the diagnostic accuracy of serum NDKA to detect advanced neoplasia (CRC or advanced adenomas). Furthermore, the performance of NDKA was compared with the faecal immunochemical test (FIT). The study population included a case-control cohort and a screening cohort (511 asymptomatic first-degree relatives of CRC patients that underwent a colonoscopy and a FIT). Serum NDKA was elevated in CRC patients in the case-control cohort (p = 0.002). In the screening cohort, NDKA levels were higher for advanced adenomas (p = 0.010) and advanced neoplasia (p = 0.006) compared to no neoplasia. Moreover, elevated NDKA was associated with severe characteristics of adenomas (≥3 lesions, size ≥ 1 cm or villous component). Setting specificity to 85%, NDKA showed a sensitivity of 30.19% and 29.82% for advanced adenomas and advanced neoplasia, respectively. NDKA combined with FIT (100 ng/mL cut-off) detected advanced adenomas and advanced neoplasia with 45.28% and 49.12% sensitivity, with specificity close to 90%. The combination of serum NDKA and FIT can improve the detection of advanced neoplasia, mainly for lesions located on the proximal colon, in asymptomatic individuals with CRC family-risk.

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Selection of putative colorectal cancer markers by applying PCA on the soluble proteome of tumors: NDK A as a promising candidate

Cited by 17 publications

References 57 publications

Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model

Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model

Proteomic and cytokine plasma biomarkers for predicting progression from colorectal adenoma to carcinoma in human patients

Evaluation of serum nucleoside diphosphate kinase A for the detection of colorectal cancer

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