Selection of rhizobacteria for biocontrol of Fusarium verticillioides on non-rhizospheric soil and maize seedlings roots

Einloft, Tiago Centeno; Hartke, Sara; Oliveira, Patrícia Bolzan de; Saraiva, Priscila Silveira; Dionello, Rafael Gomes

doi:10.1007/s10658-021-02259-y

Cited by 11 publications

(6 citation statements)

References 78 publications

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“…Over time, several bacteria are emerging as novel PGPR and declared to have biological control potentials against different fungal plant diseases. Bacillus species were reported in several studies to have an important potential as BCAs [45][46][47]. As our study highlighted several species belonging to the same genus, Ali et al [47] also reported the great ability of Bacillus tequilensis S5 to reduce the mycelial growth by 76.6%.…”

Section: Discussionsupporting

confidence: 73%

Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot

Ezrari

Mhidra

Radouane

et al. 2021

Plants

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Citrus trees face threats from several diseases that affect its production, in particular dry root rot (DRR). DRR is a multifactorial disease mainly attributed to Neocosmospora (Fusarium) solani and other several species of Neocosmospora and Fusarium spp. Nowadays, biological control holds a promising control strategy that showed its great potential as a reliable eco-friendly method for managing DRR disease. In the present study, antagonist rhizobacteria isolates were screened based on in vitro dual culture bioassay with N. solani. Out of 210 bacterial isolates collected from citrus rhizosphere, twenty isolates were selected and identified to the species level based on the 16S rRNA gene. Molecular identification based on 16S rRNA gene revealed nine species belonging to Bacillus, Stenotrophomonas, and Sphingobacterium genus. In addition, their possible mechanisms involved in biocontrol and plant growth promoting traits were also investigated. Results showed that pectinase, cellulose, and chitinase were produced by eighteen, sixteen, and eight bacterial isolates, respectively. All twenty isolates were able to produce amylase and protease, only four isolates produced hydrogen cyanide, fourteen isolates have solubilized tricalcium phosphate, and ten had the ability to produce indole-3-acetic acid (IAA). Surprisingly, antagonist bacteria differed substantially in their ability to produce antimicrobial substances such as bacillomycin (five isolates), iturin (ten isolates), fengycin (six isolates), surfactin (fourteen isolates), and bacteriocin (subtilosin A (six isolates)). Regarding the PGPR capabilities, an increase in the growth of the bacterial treated canola plants, used as a model plant, was observed. Interestingly, both bacterial isolates Bacillus subtilis K4-4 and GH3-8 appear to be more promising as biocontrol agents, since they completely suppressed the disease in greenhouse trials. Moreover, these antagonist bacteria could be used as bio-fertilizer for sustainable agriculture.

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Section: Discussionsupporting

confidence: 73%

Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot

Ezrari

Mhidra

Radouane

et al. 2021

Plants

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“…Similarly, Fallahzadeh-Mamaghani et al [ 48 ] concluded that P. polymixa N179 improved wheat crop growth and yield [ 49 ]; Maheshwari [ 50 ] obtained similar results by using biocontrol agents to successfully control early blight disease against tomato. Einloft et al [ 28 ] discovered that using a consortium of bacillus biocontrol agents can reduce the impact of F. verticillioides on maize seedlings. According to [ 51 ], in field conditions, Bacillus subtilis significantly reduced the disease incidence against F. graminearum .…”

Section: Discussionmentioning

confidence: 99%

“…The selected isolate BCB16 was grown overnight in LB broth medium and serially diluted to a concentration of 10 9 CFU/ml. Seed bacterization was carried out in accordance with Einloft et al [ 28 ], with minor modifications. For each treatment, 100 surface-sterilized wheat seeds were submerged in 250 ml bacterial inoculum (BCB16) in an orbital shaker for 2 h at 120 rpm.…”

Section: Methodsmentioning

confidence: 99%

Biocontrol rhizobacteria enhances growth and yield of wheat ( Triticum aestivum ) under field conditions against Fusarium oxysporum

Agha,

Ullah,

Khan

et al. 2023

Bioengineered

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The current study aimed to identify the survival of bio-control bacteria with antifungal activity against Fusarium oxysporum and assess their growth promoting activity in wheat crop field conditions. To evaluate the fungicidal activities of isolated bacteria using the dual culture method, both qualitative and quantitative bioassays were performed. Plant Growth Promoting activities such as Indole 3-Acetic Acid (IAA), phosphate solubilization, Hydrogen cyanide (HCN), and Siderophore production were assessed for three biocontrol bacterial isolates (BCB 07, BCB16, and BCB 83) out of 180 with 70% antagonistic activity against Fusarium oxysporum . Chitinase, protease, and cellulase interaction in isolates was also tested. BCB16 was selected as it had 70% antagonist activity against F. oxysporum but also had the highest PGPR (Plant Growth Promoting Rhizobacteria) traits when compared to the other two isolates. BCB16 was also tested for survival in talc powder and in wheat crop field conditions. Even after 4 months in talc powder, the survival rate remained stable. In a wheat crop field, BCB16 reduced the disease incidence of Fusarium oxysporum by 54.38%. When compared to fungus alone treatment, BCB16 increased average yield by 57% alone and 32% in challenged conditions. BCB16 was identified molecularly using the 16s rRNA gene. Bacillus amyloliquefaciens shared 97% of the deduced sequence. The sequence was submitted to genbank and assigned the accession number OM333889. Bacillus amyloliquefaciens has the potential to be used in the field as an alternative to synthetic fungicides against Fusarium oxysporum .

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“…The culture substrate used in this greenhouse trial consisted of 2/3 commercial peat and 1/3 sand. The substrate mixture was autoclaved twice at 121 • C for 30 min [35] in hermetically sealed plastic bags. A weight of 200 g of substrate per pot was used for testing the rhizospheric bacteria growth-promoting effect.…”

Section: Effects Of Bacterial Isolates On Sugar Beet Growth In Greenh...mentioning

confidence: 99%

Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris

El Housni,

Ezrari,

Radouane

et al. 2024

Microorganisms

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Cercospora beticola Sacc. is an ascomycete pathogen that causes Cercospora leaf spot in sugar beets (Beta vulgaris L.) and other related crops. It can lead to significant yield losses if not effectively managed. This study aimed to assess rhizosphere bacteria from sugar beet soil as a biological control agent against C. beticola and evaluate their effect on B. vulgaris. Following a dual-culture screening, 18 bacteria exhibiting over 50% inhibition were selected, with 6 of them demonstrating more than 80% control. The bacteria were identified by sequencing the 16S rRNA gene, revealing 12 potential species belonging to 6 genera, including Bacillus, which was represented by 4 species. Additionally, the biochemical and molecular properties of the bacteria were characterized in depth, as well as plant growth promotion. PCR analysis of the genes responsible for producing antifungal metabolites revealed that 83%, 78%, 89%, and 56% of the selected bacteria possessed bacillomycin-, iturin-, fengycin-, and surfactin-encoding genes, respectively. Infrared spectroscopy analysis confirmed the presence of a lipopeptide structure in the bacterial supernatant filtrate. Subsequently, the bacteria were assessed for their effect on sugar beet plants in controlled conditions. The bacteria exhibited notable capabilities, promoting growth in both roots and shoots, resulting in significant increases in root length and weight and shoot length. A field experiment with four bacterial candidates demonstrated good performance against C. beticola compared to the difenoconazole fungicide. These bacteria played a significant role in disease control, achieving a maximum efficacy of 77.42%, slightly below the 88.51% efficacy attained with difenoconazole. Additional field trials are necessary to verify the protective and growth-promoting effects of these candidates, whether applied individually, combined in consortia, or integrated with chemical inputs in sugar beet crop production.

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Selection of rhizobacteria for biocontrol of Fusarium verticillioides on non-rhizospheric soil and maize seedlings roots

Cited by 11 publications

References 78 publications

Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot

Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot

Biocontrol rhizobacteria enhances growth and yield of wheat ( Triticum aestivum ) under field conditions against Fusarium oxysporum

Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris

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