Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors

Cabañas, Keyla Tortoló; Peña-Moreno, Irina Charlot; Parente, Denise Castro; García, Antonio Bell; Gutiérrez, Roxana García; Morais, Marcos Antônio de

doi:10.1007/s13205-018-1541-3

Cited by 11 publications

(12 citation statements)

References 32 publications

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“…The aldehydes like furfural and HMF may inhibit key enzymes intervening in the rate of protein synthesis of the central metabolism of the yeasts affecting negatively the growth and fermentation (Cabañas et al 2019). Because of that, the presence of these compounds is highly unwanted in the fermentation substrate.…”

Section: Resultsmentioning

confidence: 99%

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Calegari¹,

Silva²,

Gomes³

et al. 2021

Preprint

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The presence of microbial contamination in the wort during the fermentation process results in damages of billions of dollars per year all around the world and promotes the industry dependence of chemicals and antibiotics to control the contamination. For these reasons, this study aimed to use the electron beam to sterilise wort from sugarcane molasses and investigate its bioethanol fermentation. Five treatments (T0 – T4) were carried out using ionizing doses of radiation through the electron accelerator: (0, 10, 20, 40, and 80 kGy) and a positive control (T5-steam by autoclave) were performed. It was evaluated total mesophiles, total bacteria, sugars, phenolics, flavonoids, 5-hidroximetylfurfural, and Furfural. After the irradiation process, it was conducted an alcoholic fermentation assay using baker’s yeast Saccharomyces cerevisiae. It was not observed inversion of sugars and formation of the inhibitory by-products flavonoids, furfural, and 5-hidroximetylfurfural, with exception of the phenolic compounds. Only T4 and T5 were able to sterilise the wort. However, T3 was able to eliminate > 99.99% of the microorganisms. In the fermentation T2 promoted the best ethanol yield and productivity among the irradiated treatments, evidencing the possibility of electron beam use in the wort treatment prior to fermentation which may allow a reduction in losses caused by microbial contamination, besides the possibility of promoting fermentation yield and productivity increase. The operation cost for applying the e-beam with a dose of 20 kGy in a standard ethanol plant was estimated at US$ 0.014 per m3 of wort.

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Section: Resultsmentioning

confidence: 99%

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Calegari¹,

Silva²,

Gomes³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The control setup was YNB+2% glucose without any inhibitors (Cabañas et al . 2019). The microtitre plates were incubated at 40°C, 150 rev min −1 for 48 h, and growth was measured at 600 nm with an Eon™ Microplate Spectrophotometer (BioTek ® Instruments Inc., Winooski, VT) after 48 h. All experiments were carried out in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Compost as an untapped niche for thermotolerant yeasts capable of high‐temperature ethanol production

Avchar

Lanjekar

Dhakephalkar

et al. 2021

Letters Applied Microbiology

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Efficient bioethanol production from lignocellulosic biomass requires thermotolerant yeasts capable of utilizing multiple sugars, tolerating inhibitors and fermenting at high temperatures. In this study, 98 thermotolerant yeasts were isolated from nine compost samples. We selected 37 yeasts that belonged to 11 species; 31 grew at 45°C; 6 strains grew at 47°C, while 9 yeasts could utilize multiple sugars. Many yeast isolates showed high ethanol production in the range of 12–24 g l−1, with fermentation efficiencies of 47–94% at 40°C using 5% glucose. Kluyveromyces marxianus CSV3.1 and CSC4.1 (47°C), Pichia kudriavzevii CSUA9.3 (45°C) produced 21, 22 and 23 g l−1 of ethanol with efficiencies of 83, 87 and 90%, respectively, using 5% glucose. Among these yeasts, K. marxianus CSC4.1 and P. kudriavzevii CSUA9.3 exhibited high tolerance against furfural, 5‐HMF, acetic acid and ethanol. These two strains produced high amounts of ethanol from alkali‐treated RS, with 84 and 87% efficiency via separate hydrolysis and fermentation; 76 and 74% via simultaneous saccharification and fermentation at 47 and 45°C, respectively. Therefore, this study demonstrates compost as a potential anthropogenic niche for multiple sugar‐utilizing, inhibitor‐tolerant ethanologenic yeasts suitable for high‐temperature ethanol production via SHF of rice straw.

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“…When compared with the commercial strain, TMB3720 exhibited a fourfold increase in ethanol productivity and 1.8‐fold increase in yield . Canbañas et al . also isolated eight S. cerevisiae strains from local Cuban molasses distilleries for use in cellulosic ethanol production.…”

Section: Expanding S Cerevisiae Inhibitor Resistancementioning

confidence: 99%

“…also isolated eight S. cerevisiae strains from local Cuban molasses distilleries for use in cellulosic ethanol production. The strains were evaluated for ethanol production and inhibitor resistance, and the S. cerevisiae L/25–7‐77 isolate showed promise as a future chassis for biocatalyst development …”

Section: Expanding S Cerevisiae Inhibitor Resistancementioning

confidence: 99%

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Brandt

Jansen

Görgens

et al. 2019

Biofuels Bioprod Bioref

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Lignocellulose-derived biofuels present an attractive carbon-neutral alternative to fossil fuels amidst growing global energy and climate concerns. Bioethanol in particular, has been shown to be a viable additive to and / or replacement for petroleum in countries such as Brazil. The bioconversion of lignocellulose biomass to bioethanol is a developing technology with the yeast Saccharomyces cerevisiae playing a pivotal role in the fermentation-based processes. This yeast however, is challenged to ferment under harsh conditions, specifically, during exposure to lignocellulose-derived microbial inhibitors that are formed during pretreatment processes. This detrimentally affects biocatalyst performance, ultimately decreasing ethanol productivity and yield. To this end, S. cerevisiae strains are in development to increase the yeast microbial inhibitor resistance towards cost-effective cellulosic bioethanol production. This review discusses the current status of inhibitor resistance in S. cerevisiae strains and perspectives on the future of multi-tolerant phenotypes with a specific focus on existing and emerging strain development strategies designed to improve resistance phenotypes.

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Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors

Cited by 11 publications

References 32 publications

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Compost as an untapped niche for thermotolerant yeasts capable of high‐temperature ethanol production

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

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