Selection of soybean mutant lines with altered seed coat colour and their antioxidant activity

Abstract

In this study, the flavonoid and isoflavone contents in four checks with seed coat colour altered from yellow to black were determined, and their antioxidant activity was measured. Three anthocyanins (delphinidin-3-Ob-D-glucoside, D3G; cyanidin-3-O-b-D-glucoside, C3G; and petunidin-3-O-b-D-glucoside, Pt3G) were detected in the four checks, while their controls (cvs. 'Danbaek' and 'Daepung') were not found. Among them, D-16 derived from cv. 'Danbaek' showed the highest anthocyanin contents. The isoflavone contents of all checks showed lower levels compared to their controls. Among six isoflavones, glycitein was only detected in DP-39. To analyse the radical scavenging activities among the four checks, we conducted DPPH, FRAP and UWLA assays. Significant differences in antioxidant activities were obtainable in the four checks, which contained significant differences in levels of anthocyanins and isoflavones. Based on these results, gamma irradiation may change the isoflavone and anthocyanin contents of soybean. Our four checks selected in this study may be useful for breeding soybean varieties to alter their nutritional values.

Selection of mutants with high linolenic acid contents and characterization of fatty acid desaturase 2 and 3 genes during seed development in soybean (Glycine max)

“…Gamma radiation is a useful physical mutagen for producing new traits for crop improvement . The application of gamma‐ray irradiation to generate mutants with increased linolenic acid contents has rarely been studied in soybean .…”

Identification of lignin‐deficient Brachypodium distachyon (L.) Beauv. mutants induced by gamma radiation

“…Gamma radiation as a physical mutagen is the most frequently used source of ionizing radiation for creating mutants during mutation breeding . Gamma radiation has been applied to crops to develop new varieties and to improve important agronomical traits, such as resistance to disease, pathogens, salinity and drought . By applying gamma radiation, mutants with high kernel weight have been induced in wheat, and mutants with increased chlorophyll and carotenoid content have been obtained in sugar beet .…”

“…9 Gamma radiation has been applied to crops to develop new varieties and to improve important agronomical traits, such as resistance to disease, pathogens, salinity and drought. 10,11 By applying gamma radiation, mutants with high kernel weight have been induced in wheat, and mutants with increased chlorophyll and carotenoid content have been obtained in sugar beet. 12,13 Single-nucleotide polymorphisms (SNPs) in OASA1 have been identified by targeting induced local lesions in the genome of rice mutants, created using gamma radiation, with altered seed storage proteins.…”