Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth

Kunova, Andrea; Bonaldi, M.; Saracchi, M.; Pizzatti, C.; Chen, Xiaoyulong; Cortesi, P.

doi:10.1186/s12866-016-0886-1

Cited by 78 publications

(53 citation statements)

References 57 publications

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“…The inhibitory effect of the three strains on the growth of P. infestans mycelium was assessed on LC, CS, and CFS using the plate dual culture method(29). First, a P. infestans mycelium disk (diameter = 7 mm) was placed on the center of R solid medium (diameter = 9 cm) and cultivated for three days in advance.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Wang

Zhang

et al. 2019

Preprint

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15Potato late blight triggered by Phytophthora infestans ((Mont.) de Bary) represents a 16 great food security threat worldwide and is difficult to control. Currently, Bacillus spp. 17 have been considered biocontrol agents to control many fungal diseases. Here, 18 Bacillus subtilis WL-2 was selected as the antifungal strain with the most potential 19 against P. infestans mycelium growth. Additionally, the functional metabolites 20 extracted from WL-2 were identified as IturinA-family cyclic lipopeptides (CLPs) via 21 2 high-performance liquid chromatography (HPLC) and electrospray ionization mass 22 spectrometry (ESI-MS). Analyses using scanning and transmission electron 23 microscopy (SEM and TEM) revealed that IturinA caused a change in the mycelial 24 surface and damage to the internal cell structure, including cell membrane disruption 25 and irregular organelle formation. Moreover, propidium iodide staining and nucleic 26 acid and protein release were detected to clarify the cell membrane damage caused by 27 IturinA. Additionally, IturinA triggered reactive oxygen species (ROS) generation and 28 malondialdehyde (MDA) production. Mitochondrial membrane potential (MMP), 29 mitochondrial respiratory chain complexes activity (MRCCA), respiratory control rate 30 (RCR), and oxidative phosphorylation efficiency (P/O) assays indicated that P. 31 infestans mitochondria affected by IturinA were so seriously damaged that the MMP 32 and MRCCA declined remarkably and that mitochondrial ATP production ability was 33 weakened. Therefore, IturinA induces cell membrane damage, oxidative stress, and 34 dysfunction of mitochondria, resulting in P. infestans hyphal cell death. As such, the 35 results highlight that B. subtilis WL-2 and IturinA have great potential as candidates for 36 inhibiting P. infestans mycelium growth and controlling potato late blight. 37 IMPORTANCE 38Potato (Solanum tuberosum L.) is the fourth most common global food crop, and 39 its planting area and yield increase yearly. Notably, in 2015, China initiated a potato 40 staple food conversion strategy, and by 2020, approximately 50% of potatoes will 41 be consumed as a staple food. The plant pathogen fungus Phytophthora infestans 42 ((Mont.) de Bary) is the culprit of potato late blight; however, biological agents 43 3 rather than chemicals are highly necessary to control this threatening disease. In 44 this study, we discovered an antifungal substance, IturinA, a lipopeptide produced 45 by Bacillus subtilis WL-2. Moreover, our research revealed the actual mechanism 46 of IturinA against P. infestans mycelium growth and clarified the potential of B. 65Behind rice, wheat, and corn, potato (Solanum tuberosum L.) is the fourth most 66 stable food crop in the world. However, potato production is often endangered by 67 many pathogens. Worryingly, late blight triggered by Phytophthora infestans ((Mont.) 68 de Bary) could directly reduce or even eliminate potato production, and an outbreak 69 of this disease could even result in a grievous ...

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Section: Methodsmentioning

confidence: 99%

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Wang

Zhang

et al. 2019

Preprint

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“…The prepared soils were labelled as natural soil and sterile soil [ 29 – 30 ]. Two treatments for the different soil types were carried out: the inoculation of 5 mL of only the Streptomyces sp.…”

Section: Methodsmentioning

confidence: 99%

Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere

et al. 2018

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Streptomyces species 1–14 isolated from cassava rhizosphere soil were evaluated for their antibacterial efficacy against Fusarium oxysporum f.sp. cubense race 4 (FOC4). Of the 63 strains tested, thirteen exhibited potent antibacterial properties and were further screened against eight fungal pathogens. The strain that showed maximum inhibition against all of the test pathogens was identified by 16S rDNA sequencing as Streptomyces sp. 1–14, was selected for further studies. Through the propagation of Streptomyces sp. 1–14 in soil under simulated conditions, we found that FOC4 did not significantly influence the multiplication and survival of Streptomyces sp. 1–14, while indigenous microorganisms in the soil did significantly influence Streptomyces sp. 1–14 populations. To achieve maximum metabolite production, the growth of Streptomyces 1–14 was optimized through response surface methodology employing Plackett-Burman design, path of steepest ascent determinations and Box-Behnken design. The final optimized fermentation conditions (g/L) included: glucose, 38.877; CaCl2•2H2O, 0.161; temperature, 29.97°C; and inoculation amount, 8.93%. This optimization resulted in an antibacterial activity of 56.13% against FOC4, which was 12.33% higher than that before optimization (43.80%). The results obtained using response surface methodology to optimize the fermentation medium had a significant effect on the production of bioactive metabolites by Streptomyces sp. 1–14. Moreover, during fermentation and storage, pH, light, storage temperature, etc., must be closely monitored to reduce the formation of fermentation products with reduced antibacterial activity. This method is useful for further investigations of the production of anti-FOC4 substances, and could be used to develop bio-control agents to suppress or control banana fusarium wilt.

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“…Recently streptomycetes are gaining interest in agriculture as PGP bacteria and BCA (Kunova et al . ). Streptomycetes are commonly found in soil and are able to colonize rhizosphere and root tissues (Cao et al .…”

Section: Introductionmentioning

confidence: 97%

Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria

Thilagam

Hemalatha

2019

J Appl Microbiol

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Aim The aim of this study was to screen potential plant growth promoting rhizobacterial (PGPR) actinobacterial isolate with effective inhibition against anthracnose causing fungal pathogen Colletotrichum capsici. Methods and Results In this study, actinobacterias were isolated from rhizosphere soil using dilution plate method and tested for antagonistic potential against pathogenic fungi C. capsici. In primary and secondary screening tests, the actinobacterial isolate BS‐26 displayed high antagonistic activity against the fungal pathogen. Isolate BS‐26 was identified as Streptomyces violaceoruber based on 16S rDNA sequencing. Furthermore, indole acetic acid production, phosphate solubilization and ammonia production have been confirmed in the S. violaceoruber that suggest their potential to be used as PGPR bacteria. A green house experiment showed that application of S. violaceoruber fermentation broth reduced the incidence of the chilli anthracnose and promoted the growth of chilli seedlings with a significant increase in germination %, total plant height, fresh weight and chlorophyll content when compared to controls. Conclusion Streptomyces violaceoruber can be applied as a biofertilizer and biocontrol agent for growing chillies against the attack of fungal pathogen C. capsici. Significance of Impact of the Study The damage caused by anthracnose disease is an issue of concern, affecting negatively the economy involved in chilli cultivation. As chemical methods of control have serious disadvantages, biocontrol approach using beneficial (PGPR) micro‐organisms shall be a better alternative to control crop diseases.

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Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth

Cited by 78 publications

References 57 publications

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere

Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria

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