Selection of the optimal day for oocyte retrieval based on the diameter of the dominant follicle in hCG-primed in vitro maturation cycles

Son, Weon‐Young; Chung, Jin‐Tae; Herrero, Belen; Dean, Nicola; Demirtaş, Ezgi; Holzer, Hananel; Elizur, Shai E.; Chian, Ri‐Cheng; Tan, Seang Lin

doi:10.1093/humrep/den332

Cited by 91 publications

(82 citation statements)

References 22 publications

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“…In addition, they also did not mention whether they used the same criteria for triggering hCG as their previous study or not. We have previously reported that there were no differences in the rates of clinical pregnancy and implantation between the IVM cycles where embryos were transferred with and without embryos produced from in vivo matured oocytes when hCG priming was administered when the DF reached 10-12 mm, even though the study groups were for women who had polycystic ovaries [18]. Our present data is also consistent with that of Paulson et al [19], who reported that all pregnancies occurred in the cycles in which one of the embryos was derived from the DF (17 %, 11/66) in unstimulated IVF cycles of women with regular cycles and all of the implantations were singleton.…”

Section: Discussionmentioning

confidence: 93%

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Son

Chung

Das

et al. 2012

J Assist Reprod Genet

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Purpose To analyze the fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle IVF in women with regular cycles. Methods Natural cycle IVF was performed in 28 patients who had normal ovaries, > 6 antral follicle counts and were less than 40 years old (n028 cycles). An hCG trigger of 10,000 IU was administered 36 h before oocyte collection when the diameter of the dominant follicle (DF) was over 12 mm. Oocytes were retrieved from DF as well as from the cohort of smaller follicles. Embryological aspects of the mature and immature oocytes retrieved from these cycles as well as the implantation and clinical pregnancy rates depending on the origin of the embryos transferred were evaluated. Result(s) Overall clinical pregnancy and implantation rates were 20.8 % and 6.7 %, respectively. There were no differences in in vitro maturation (IVM), fertilization and embryo development between immature oocytes retrieved with and without in vivo matured oocytes. However, the clinical and implantation rates in cycles with embryos produced from in vivo matured oocytes transferred were better than the cycles where only IVM embryos were transferred (30.8 %, 9.1 % vs. 9.1 %, 3.2 %). Conclusion(s) Although our results show that immature oocytes from natural cycle IVF can fertilize normally and can be used to increase the number of embryos available for transfer, the embryos derived from the immature oocytes in natural cycles IVF have a poorer reproductive potential.

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Section: Discussionmentioning

confidence: 93%

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Son

Chung

Das

et al. 2012

J Assist Reprod Genet

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“…Ovarian tissue cryopreservation (OTC) is an alternative protocol that has attracted interest due to the fact that the only precondition for its application is the presence of ovaries, while variables such as age [9], menstrual cycle phase [12,25] and subjacent disease do not have a bearing on whether or not it can be performed. In addition, the tissue reimplantation that follows depends purely on the absence of malignant cells [2,13,20,21,24].…”

Section: Introductionmentioning

confidence: 99%

“…Retrieval of immature oocytes from unstimulated ovaries followed by IVM is an effective treatment option for many infertile women [4,5,11,25] and cancer sufferers. In oncologic patients, oocytes can be retrieved by means of standard oocyte aspiration [12,15] or in association with OTC [9,10,12,19].…”

Section: Introductionmentioning

confidence: 99%

Spontaneous in vitro maturation of oocytes prior to ovarian tissue cryopreservation in natural cycles of oncologic patients

Escribá

Grau

Escrich

et al. 2012

J Assist Reprod Genet

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Purpose To evaluate the recovery rate and spontaneous in vitro maturation (IVM) of immature oocytes enclosed within or released from follicles during the processing of ovarian tissue prior to its cryopreservation. Methods Thirty-three oncologic patients who had not previously undergone chemo or radiotherapy underwent ovarian tissue cryopreservation (OTC) during natural menstrual cycles. Immature oocytes, enclosed within follicles or released during ovarian cortex processing, were collected and matured spontaneously in vitro for 48 h. Nuclear maturation was assessed every 24 h and the ability of the IVM oocytes to display a normal activation response following parthenogenetic activation was evaluated. The following outcome measures were also evaluated: disease, age, FSH, LH, E2, P4 and AMH serum levels, menstrual cycle day, recovery and spontaneous IVM and parthenogenetic activation rates. Results Oocytes recovered per patient were 3.3±0.7 (1.8-4.7 oocytes, 95CI), regardless of the menstrual phase. The mean number of IVM oocytes per patient was 1.3±0.2 oocytes (95CI: 0.8-1.8), regardless of menstrual phase (p0 0.86) and oocyte origin (p00.61). Forty-one percent of oocytes extruded the second polar body and formed one pronucleus after parthenogenetic activation. Conclusion Twenty-one of the 33 women (63.6 %) requesting OTC produced at least one mature oocyte.

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“…Despite the limited number of oocytes obtained by aspiration (3.3 in average), clinical pregnancy rate of 36.4% was achieved, which is between the 17 to 41% rate that is reported for this technique. Eventhough there are reports that claim an advantage in the administration of hCG before obtaining the oocytes (Son et al, 2008a, 2008b, Tan & Child, 2002, Chian, 2004 it's being also manifested that hCG interrupts comunication between follicular cells and the oocyte; this is an important event to the adquisition of a good embryonary potential development (Albertini et al, 2001). In our IVM protocol, we do not use hCG in vivo.…”

Section: Discussionmentioning

confidence: 99%

In vitro Maturation of Oocytes in High Altitude Women with Polycystic Ovaries

Tominaga¹,

Pella²,

Lechuga³

et al. 2015

JBRA Assisted Reproduction

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Objective: Determine the effectiveness of in vitro maturation of oocytes in the infertility treatment in high altitude women with polycystic ovaries. Methods: Design: descriptive and retrospective study. Participants: Women with polycystic ovaries and infertility. Interventions: there were 11 women from locations above 7,546 feet above sea level with polycystic ovaries and infertility in which were performed in vitro maturation of oocytes, followed by intracytoplasmic sperm injection, culture and embryo vitrification. After that, the endometrium was prepared and the embryos were thawed and transferred. Main results mesurements: oocytes maturation, fertilization, clinical pregnancy and implantation rates. Results: Oocytes maturation rate was 86.1%; fertilization rate 90.3%; clinical pregnancy rate 36.4% and implantation rate 17.4%. Conclusions: In vitro maturation of oocytes is an effective technique in the infertility treatment of high altitude women with polycystic ovaries.

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Selection of the optimal day for oocyte retrieval based on the diameter of the dominant follicle in hCG-primed in vitro maturation cycles

Abstract: Our results suggest that oocyte collection in IVM cycles should be performed when the DF is 14 mm diameter or less. Sibling immature oocytes may be affected detrimentally if a DF >14 mm is present at oocyte collection.

Cited by 91 publications

References 22 publications

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Spontaneous in vitro maturation of oocytes prior to ovarian tissue cryopreservation in natural cycles of oncologic patients

In vitro Maturation of Oocytes in High Altitude Women with Polycystic Ovaries

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