Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production

Araque, Edgardo; Parra, Carolina; Manuel, Rodríguez; Freer, Juanita; Baeza, Jaime

doi:10.1016/j.enzmictec.2008.02.007

Cited by 118 publications

(70 citation statements)

References 19 publications

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“…Whereas, unsuitable temperature of cellulase enzymatic hydrolysis will supplement the difficulty of hydrolysing substrate to fermentable sugar. To overcome these problems, many researchers used the SSF in fed-batch mode to maintain the low level of viscosity in slurry [2], screened and induced yeast to fermentation at high temperature, and used genitic engineering to thermo-tolerant yeast [5,9,10].…”

Section: Introduction mentioning

confidence: 99%

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Srinorakutara¹,

Subkaree²,

Boonvitthya³

et al. 2015

JFSE

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Effect of commercial cellulose enzymes was investigated by batch enzymatic hydrolysis at 15.0% (w/v) solid. It was found that the best commercial cellulose enzyme was Cellic ® CTec comparing to Accellerase 1000 TM and Accelerase 1500 TM . The Cellic ® CTec gave the highest reducing sugar concentration and rice straw conversion. Moreover, when the hydrolysate obtained from hydrolysis using Cellic ® CTec was fermented by Saccharomyces cerevisiae TISTR 5596, it would give the highest ethanol. In this study, the Cellic ® CTec was used for fed-batch prehydrolysis prior to ethanol production by simultaneous saccharification and fermentation (SSF) way at 20% (w/v) solid loading. It could produce 35.76 g/L or 4.6% (v/v) of ethanol concentration and 83.67 L/ton dry matter (DM) of yield.

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Section: Introduction mentioning

confidence: 99%

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Srinorakutara¹,

Subkaree²,

Boonvitthya³

et al. 2015

JFSE

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“…Saccharomyces cerevisiae is naturally mesophilic, but fermentation is an exothermic process. As a result, the viability of the cells will be severely repressed by the increased temperature in the reaction vessel during fermentation (Edgardo et al 2008). Thermotolerant yeast was needed for SScF at high temperatures.…”

Section: Effect Of Inhibitors On the Hybrid Strains And Haploid Straimentioning

confidence: 99%

Hybridization Improves Inhibitor Tolerance of Xylose-fermenting Saccharomyces cerevisiae

Li¹,

Zhu²,

Li³

et al. 2017

BioResources

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Although some engineered S. cerevisiae strains exhibit good xylose utilization ability, the lack of tolerance to inhibitors generated in biomass pretreatment limits the application of such strains in the production of bioethanol from lignocellulosic biomass. By applying a sexual mating method, inhibitor tolerance was developed in xylose-utilizing strains. The final ethanol concentrations in simultaneous scarification and cofermentation (SScF) process at 38 °C with hybrid strains were 50% higher than the SScF process with the xylose-fermenting parent strain. The strain viability of the hybrid strain E7-12 at 24 h was 282 times higher than the parent strain in the SScF process at 25% solid loading. Due to the improved sugar utilization, the final ethanol concentration reached 69.7 g/L (E7-11) and 70.0 g/L (E7-12), which were 25.3 g/L and 25.6 g/L higher than that of SScF with the xylose-fermenting strain, respectively.

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“…The Saccharomyces cerevisiae yeast has proved to be more robust than bacteria, being more tolerant to ethanol and inhibitors present in hydrolysates of lignocellulosic materials [4]. Therefore, It is the most employed microorganism for fermenting the hydrolysates of lignocellulosic biomass.…”

Section: Microorganisms and Preparation For Inoculationmentioning

confidence: 99%

“…The spore suspension of Fungi containing 1-1.5×10 7 spores/mL was used as inoculums for the production of cellulase enzyme. Liquid medium for the preparation of the yeast was composed of 50 g glucose, 5 g yeast extract, 1 g KH 2 PO 4 , 0.3 g NH 4 Cl and 2 g MgSO 4 .7H 2 O per liter [5]. After yeast inoculation from the slant to this environment, it was placed in shaker at 41˚C and 130 rpm for 24 hours.…”

Section: Microorganisms and Preparation For Inoculationmentioning

confidence: 99%

See 1 more Smart Citation

Ethanol production from sugarcane bagasse by means of on-site produced and commercial enzymes; a comparative study

Shaibani

Yaghmaei

Andalibi

et al. 2012

Per. Pol. Chem. Eng.

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Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production

Cited by 118 publications

References 19 publications

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Hybridization Improves Inhibitor Tolerance of Xylose-fermenting Saccharomyces cerevisiae

Ethanol production from sugarcane bagasse by means of on-site produced and commercial enzymes; a comparative study

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