Selection of tolerant cells from carrot suspension culture to bensulfuron methyl, glyphosate and glufosinate.

Suwanwong, Srisom; Usui, Kenji; Ishizuka, Kôzô

doi:10.3719/weed.34.315

Cited by 3 publications

(5 citation statements)

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“…The enzyme activity was inhibited by 50% at ≈8 × 10 −10 mol L −1 and severe inhibition was observed at concentrations > 10 −7 mol L −1 . These results were consistent with those of the previous paper, in which the response to BSM was demonstrated in the plant‐cultured cells (Suwanwong et al . 1988; Watanabe et al .…”

Section: Resultssupporting

confidence: 94%

“…The growth inhibition by BSM has also been reported in carrot and rice cells (Watanabe et al . 1988; Suwanwong et al . 1989; Sengnil et al .…”

Section: Resultsmentioning

confidence: 99%

“…1991). Furthermore, the response of carrot and rice to BSM was also investigated by means of plant tissue cultures (Suwanwong et al . 1989; Watanabe et al .…”

Section: Introductionmentioning

confidence: 99%

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Growth inhibition and acetolactate synthase activity of soybean seedlings and suspension‐cultured cells treated with bensulfuron‐methyl

Pornprom

Usui

Ishizuka

2005

Weed Biology and Management

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Keywords: acetolactate synthase, bensulfuron-methyl, growth inhibition, soybean seedlings, soybean suspension-cultured cells.activity for a better understanding of the relationship between the inhibition of ALS activity and the growth response. MATERIALS AND METHODS Plant materialsSoybean (Glycine max L. cv. Enrei) was grown on MS medium (Murashige and Skoog 1962) and its suspension-cultured cells were established and maintained in MS medium, as described previously (Pornprom et al. 1994). ChemicalsTechnical-grade BSM (methyl 2-[[[[(4,6-dimethoxypyrimidin-2-yl) amino] carbonyl] amino] sulfonyl] methyl] benzoate; 96% purity), the gift of Du Pont, Tokyo, Japan, was dissolved in sodium-phosphate buffer (pH = 8.04) and applied as sterile, filtered solutions. All the other chemicals were purchased from Wako Pure Chemical Industries, Osaka, Japan. Inhibition of growth by bensulfuron-methylThe surface-sterilized soybean seeds were placed in 1/2 strength MS medium containing different concentrations of herbicide (10 -10 -10 -6 mol L -1 BSM) at 27∞C under dim light (ª30 mEm -2 s -1 ) for 10 days. Then, the germination rates of the seeds and the fresh weight of the soybean seedlings were measured.Soybean suspension-cultured cells were maintained under dim light at 27∞C and were subcultured every 10 days at the linear phase of the growth cycle. Then,

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Section: Resultssupporting

confidence: 94%

“…The growth inhibition by BSM has also been reported in carrot and rice cells (Watanabe et al . 1988; Suwanwong et al . 1989; Sengnil et al .…”

Section: Resultsmentioning

confidence: 99%

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Growth inhibition and acetolactate synthase activity of soybean seedlings and suspension‐cultured cells treated with bensulfuron‐methyl

Pornprom

Usui

Ishizuka

2005

Weed Biology and Management

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“…Based on the 150 value, the cells were 100-fold more tolerant than normal cells to oxyfluorfen. The result may indicate the possibility of isolating native tolerant cells from a heterogeneous mixture during exposure to this herbicide11, 14,15,19,20).…”

Section: Discussionmentioning

confidence: 99%

Selection for Herbicide Tolerance in Soybean Using Cell Suspension Culture.

Pornprom¹,

Usui²,

Ishizuka³

1994

J. Weed Sci. Tech

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“…Several papers describe the procedures of glufosinate‐resistant cell selection and regeneration from the variant cells (Ishida et al . 1989; Suwanwong et al . 1989; Pornprom et al .…”

Section: Introductionmentioning

confidence: 99%

Increased activity and reduced sensitivity of glutamine synthetase in glufosinate‐resistant vetiver (Vetiveria zizanioides Nash) cells

Prasertsongskun¹,

Sangduen²,

Suwanwong³

et al. 2002

Weed Biology and Management

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Vetiver (Vetiveria zizanioides Nash) cells derived from an inflorescence were cultured in a modified N6 liquid medium supplemented with 10 µm 2,4‐D and 10 mm proline. Exponentially growing cell suspensions were subcultured with a selection medium containing glufosinate (ammonium dl‐homoalanin‐4‐yl(methyl)phosphinate). The glufosinate‐resistant cells which can grow in a medium containing 5 × 10−5 M glufosinate was selected by a stepwise selection, and its I50 value was determined to be 4.2 × 10−5 M. The growth of susceptible cells was inhibited by lower concentrations of glufosinate and its I50 value was 2.5 × 10−7 M. This indicated that the selected cells were 170‐fold resistant compared with the susceptible cells. Glutamine synthetase (GS) activity of the resistant cells was twice as high as that of the susceptible cells. The I50 values of glufosinate were 3.2 × 10−5 M and 9.0 × 10−7 M for GS from the resistant and susceptible cells, respectively. The accumulation of ammonia caused by GS inhibition was higher in the susceptible cells. Absorption of [3,4–14C]glufosinate was not significantly different between the resistant and susceptible cells. Both cell types did not metabolize glufosinate. These results suggest that the resistance of the selected vetiver cell suspension to glufosinate is mainly due to increased GS activity and its decreased sensitivity to the herbicide.

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Selection of tolerant cells from carrot suspension culture to bensulfuron methyl, glyphosate and glufosinate.

Cited by 3 publications

References 0 publications

Growth inhibition and acetolactate synthase activity of soybean seedlings and suspension‐cultured cells treated with bensulfuron‐methyl

Growth inhibition and acetolactate synthase activity of soybean seedlings and suspension‐cultured cells treated with bensulfuron‐methyl

Selection for Herbicide Tolerance in Soybean Using Cell Suspension Culture.

Increased activity and reduced sensitivity of glutamine synthetase in glufosinate‐resistant vetiver (Vetiveria zizanioides Nash) cells

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