Selection of tomato genotypes resistant to tomato leaf curl virus disease using biochemical and physiological markers

Abstract

SUMMARYA total of 34 tomato genotypes (24 F1+10 parents) were tested for resistance to tomato leaf curl virus (ToLCV) disease by various whitefly inoculation techniques under field and insect-proof glasshouse (mass and cage) conditions. Of the ten tomato parental lines, two accessions [EC-520061 (Solanum habrochaites) and EC-521080 (Solanum pimpinellifolium)] were identified as highly resistant while four accessions (EC-520049, EC-528372, WIR-5032 and WIR-3957) of wild species were resistant to ToLCV. Out of the 24 F1 crosses, PBC×EC-520061, H-86×EC-520061, H-24×EC-520061 and DVRT-2×EC-520061 were found to be highly resistant against ToLCV disease. Biochemical (total phenol and total sugar concentration) and physiological (chlorophyll content and leaf area index) parameters were also used in healthy and disease-inoculated leaves of ten parents and six F1 hybrids to test the conformity of ToLCV-resistant and susceptible disease reactions. The results showed that among the 16 genotypes (10 parents+6 F1), EC-520061, EC-520049, PBC×EC-520061 and H-86×EC-520061 were stable for both biochemical and physiological markers while EC-521080 showed higher accumulations of total phenol and sugar concentrations and reduced leaf size between healthy and disease-inoculated leaves. The present study demonstrates the importance of the whitefly inoculation technique and biochemical and physiological markers in virus resistance screening programmes, and identifies a potential source of resistance to the ToLCV in Solanum species.

Effect of Environmental Factors on Biochemical Properties of Tomato Leaf Curl Virus Infected Leaves of Tomato

“…Bertamini et al, (2002) and Hemida (2005) observed almost similar outcomes with our findings that chlorophyll contents of virus infected leaves were found to be reduced at maximum temperature. Our experimental results are also comparable with Singh et al, (2014) who investigated that occurrence of TLCV has a significant effect on chlorophyll concentration in infected host plant which exhibit very close association among susceptible and resistant genotypes. Chlorophyll concentration reduced in infected virus plants at high temperature has been studied many scientists which might be due to chlorosis which are more frequently occurred in the infected leaves.…”

Untangling the Physio-Chemical and Transcriptional Changes of Black Gram Cultivars After Infection With Urdbean Leaf Crinkle Virus

“…From this, we conclude that the viral infection increased the sugar accumulation and decreased the photosynthetic properties (chlorophyll "a" and "b"). The increase or decrease in VBN (Bg) 6 is not very high and it indicates that the nature of moderate resistance in VBN (Bg) 6 is due to its better photosynthetic properties and less accumulation of sugar, and our findings agree with the statements of Singh et al (2015), Souza et al (2017), andPatwa et al (2020).…”

Phenotypic evaluation and utilization of tomato germplasm resistance for ToLCV and its vector Bemisia tabaci under natural condition

“…Similar findings were given for Arka vikas by [13] as a susceptible variety with 76.67 PDI. Based on the coefficient of infection, [14] screened nine tomato genotypes (viz., EC-520049 (S. chmielewskii), EC-520058, EC-520060 and EC-520061 (S. habrochaites), EC-520070, EC-520071, EC-520077, EC-520079 (S. pimpinellifolium) and H-88-78-1(S. lycopersicum; a derivative of S. habrochaites f. glabratum) and were found to be highly resistant. The results are further supported by [15] , who screened thirty five tomato genotypes including wild accessions for its resistance/susceptible reaction against tomato leaf curl disease in field condition during summer cropping season 2015.…”