Selection of variant hepatoma cells in liver-specific growth media: regulation at the mRNA level

AMP-activated Protein Kinase Mediates Phenobarbital Induction of CYP2B Gene Expression in Hepatocytes and a Newly Derived Human Hepatoma Cell Line

“…Isolation of WGA Hepatoma Cells (a Differentiated Variant of HepG2)-The WGA cell population was isolated from HepG2 after metabolic selection as described by Armbruster et al (26) with some modification. HepG2 cells were first adapted to a new growth medium consisting of Leibovitz's L15 medium (Invitrogen) supplemented with 2% Ultroser G (Biosepra S.A., Cergy-Saint-Christophe, France).…”

Liver fructose-1,6-bisphosphatase cDNA: trans-complementation of fission yeast and characterization of two human transcripts

“…They should provide some additional modulation to a key enzyme that is otherwise subject to (1) acute allosteric regulation of adenosine monophosphate (AMP) and fructose 2,6-bisphosphate [ 121, and (2) long-term transcriptional gene regulation involving cyclic AMP (CAMP) and insulin [ 1 11. Fru-l,6-Pase and the well-characterized gluconeogenic phosphoenolpyruvate carboxykinase gene [5] will be the target of trans-activation experiments aimed at the cloning of cDNAs that control liver differentiation [2], in which the rat liver pcD-Fru-1,6-Pase minigene that we have isolated will be used in ancillary experiments.…”

“…Total RNA was thus extracted from human kidney cortex and from human intestinal and hepatic cell cultures. Hepatic cells comprised primary-cultured hepatocytes and, as negative controls, two well-differentiated subclones of the nongluconeogenic hepatoblastoma HepG2 line [2]. The colon carcinoma Caco-2 line provided for both undifferentiated and well-differentiated human enterocytic cells depending on culture conditions; Caco-2 differentiation proceeds upon long-term maintenance of confluent cultures [42].…”