Selective Action Mechanism of Fenclorim on Rice and <i>Echinochloa crusgalli</i> Is Associated with the Inducibility of Detoxifying Enzyme Activities and Antioxidative Defense

Hu, Lifeng; Huang, Yajie; Ding, Bowen; Cai, Ruwen; Bai, Lianyang

doi:10.1021/acs.jafc.1c00550

Cited by 17 publications

(17 citation statements)

References 29 publications

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“…48 In addition, the GST activity analysis showed that fenclorim increased the activity of rice instead of E. crus-galli. 48 Some studies have already reported the risk to select resistant weed biotypes applying herbicide safeners. Cloquintocet-mexyl and mefenpyr-diethyl reduced Agrostis stolonifera injury from pinoxaden, but also reduced efficacy against Poa trivialis, and did not affect Lolium perenne control.…”

Section: Discussionmentioning

confidence: 99%

“…48 The transcriptome analysis showed that fenclorim up-regulated six GST genes in rice, while it down-regulated four GST genes in E. crus-galli. 48 In addition, the GST activity analysis showed that fenclorim increased the activity of rice instead of E. crus-galli. 48 Some studies have already reported the risk to select resistant weed biotypes applying herbicide safeners.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of this safener occurs for multiple crops for multiple herbicide modes of action, such as rice to fenoxaprop‐ p ‐ethyl, 45 corn to foramsulfuron 46 and tembotrione 47 . Another safener, fenclorim, can protect rice from injury when sprayed with the herbicide pretilachlor, but it does not protect E. crus‐galli 48 . The transcriptome analysis showed that fenclorim up‐regulated six GST genes in rice, while it down‐regulated four GST genes in E. crus‐galli 48 .…”

Section: Discussionmentioning

confidence: 99%

“…Another safener, fenclorim, can protect rice from injury when sprayed with the herbicide pretilachlor, but it does not protect E. crus‐galli 48 . The transcriptome analysis showed that fenclorim up‐regulated six GST genes in rice, while it down‐regulated four GST genes in E. crus‐galli 48 . In addition, the GST activity analysis showed that fenclorim increased the activity of rice instead of E. crus‐galli 48 …”

Section: Discussionmentioning

confidence: 99%

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The safener isoxadifen‐ethyl confers fenoxaprop‐p‐ethyl resistance on a biotype of Echinochloa crus‐galli

Cutti

Rigon

Girelli

et al. 2022

Pest Management Science

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BACKGROUND: Some herbicides are commercially formulated with safeners to increase crop selectivity. Fenoxaprop-p-ethyl is formulated with the safener isoxadifen-ethyl for Echinochloa crus-galli control in rice. Safeners act on crops by increasing herbicide metabolism, but this effect may also occur in weeds. The objective of this study was to investigate the effect of the safener isoxadifen-ethyl on the resistance to fenoxaprop-p-ethyl in a biotype of E. crus-galli.RESULTS: A screening of 52 biotypes identified lack of control in the biotype SANTPAT-R treated with the recommended dose of 69 g ha −1 of the commercial formulation of fenoxaprop-p-ethyl with the safener isoxadifen-ethyl. While this biotype survived doses greater than 2208 g ha −1 of the formulation fenoxaprop-p-ethyl + isoxadifen-ethyl, it was killed with 69 g ha −1 of fenoxaprop-p-ethyl without the safener. A glutathione-s-transferase (GST) enzymes inhibitor reduced the resistance factor in two dose-response curves. A minor effect of a CytP450 inhibitor was observed. The previous spraying of the safener isoxadifen-ethyl followed by fenoxaprop-p-ethyl induced survival in the resistant but not in the susceptible biotype. The GST1 and GSTF1 genes were up-regulated in the resistant biotype. ACCase gene mutations were not found, and no cross-resistance to other ACCase inhibitors was identified.CONCLUSION: The safener isoxadifen-ethyl present in the commercial herbicide formulation of fenoxaprop-p-ethyl is associated with resistance in the E. crus-galli SANTPAT-R biotype. This resistance is related with herbicide metabolization mediated by GST pathways. This is the first field-selected weed biotype with herbicide resistance due to safener presence in the sprayed formulation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The safener isoxadifen‐ethyl confers fenoxaprop‐p‐ethyl resistance on a biotype of Echinochloa crus‐galli

Cutti

Rigon

Girelli

et al. 2022

Pest Management Science

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“…Herbicides are an efficient tool in modern agriculture and effectively control weeds in the crop field [11,12]. Generally, herbicides have a specific site of action that kills weeds by selecting the potential molecular target.…”

Section: Introductionmentioning

confidence: 99%

Searching of Novel Herbicides for Paddy Field Weed Management—A Case Study with Acetyl-CoA Carboxylase

Antony

Ramanathan

2022

Agronomy

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Weed management is the major biological constraint in paddy (Oryza sativa L.) producing areas. Predominantly, barnyard grass (Echinochloa crus-galli) is a rice-mimicking weed that causes 57% of yield loss in rice production. Conventionally, herbicides are the site-specific weed inhibitors often used to suppress E. crus-galli growth. Acetyl-CoA carboxylase (ACCase) is an important target for developing novel herbicides with remarkable selectivity against gramineous weeds. Notably, fenoxaprop-P-ethyl (FPPE) is a selective ACCase herbicide extensively used in paddy fields to inhibit barnyard grass. However, prolonged use of FPPE herbicide elicits phytotoxicity in cultivated rice and herbicide resistance in weeds. Recently, phytotoxins are emerging as an alternative to commercial herbicides with safer environmental profiles. Nevertheless, discovering natural herbicides through in vivo and in vitro techniques is time-consuming and expensive. Therefore, high-end computational screening strategies including Tanimoto similarity, docking, binding free energy, and herbicide-likeness were used to pinpoint the lead molecule. Finally, molecular dynamics and MM/PBSA calculations were employed to validate the binding kinetics of the hit compound. Indeed, sinigrin was identified as a promising phytotoxic inhibitor against the ACCase enzyme. The findings of our study were well correlated with the existing experimental results. Overall, the current work will aid in the development of commercializing phytotoxin herbicides in foreseeable future.

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Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop‐P‐methyl resistance in Digitaria sanguinalis

Liu

Hou

Zhang

et al. 2023

Pest Management Science

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BackgroundHaloxyfop‐P‐methyl, an acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicide, has been extensively used to control grass weeds. Widespread use of haloxyfop‐P‐methyl in cotton fields in China has led to the development of glutathione transferase (GST)‐mediated resistance in Digitaria sanguinalis. An RNA‐seq analysis identified DsGSTU1, a tau class glutathione transferase from the D. sanguinalis transcriptome as a potential candidate. Here, we cloned DsGSTU1 from D. sanguinalis young leaf tissues and subsequently characterized DsGSTU1 by a combination of sequence analysis, as well as functional heterologous expression in rice.RESULTSThe full‐length coding DNA sequence (CDS) of DsGSTU1 is 717 bp in length. Higher DsGSTU1 expression was observed in haloxyfop‐P‐methyl‐resistant (HR) D. sanguinalis than in haloxyfop‐P‐methyl‐susceptible (HS) plants. Overexpression of the DsGSTU1 gene was confirmed by transformation into the wild‐type (WT) Nipponbare rice with pBWA(V)HS, a recombinant expression vector. GST activity in transgenic rice seedlings was 1.18–1.40‐fold higher than the WT rice seedlings before and after haloxyfop‐P‐methyl treatment, respectively. Additionally, transgenic rice seedlings overexpressing DsGSTU1 were less sensitive to haloxyfop‐P‐methyl.CONCLUSIONOur combined findings suggest that DsGSTU1 is involved in metabolic resistance to haloxyfop‐P‐methyl in D. sanguinalis. A better understanding of the major genes contributing to herbicide‐resistant D. sanguinalis facilitates the development of resistance management strategies for this global invasive grass weed. © 2023 Society of Chemical Industry.

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Selective Action Mechanism of Fenclorim on Rice and Echinochloa crusgalli Is Associated with the Inducibility of Detoxifying Enzyme Activities and Antioxidative Defense

Cited by 17 publications

References 29 publications

The safener isoxadifen‐ethyl confers fenoxaprop‐p‐ethyl resistance on a biotype of Echinochloa crus‐galli

The safener isoxadifen‐ethyl confers fenoxaprop‐p‐ethyl resistance on a biotype of Echinochloa crus‐galli

Searching of Novel Herbicides for Paddy Field Weed Management—A Case Study with Acetyl-CoA Carboxylase

Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop‐P‐methyl resistance in Digitaria sanguinalis

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