Selective adsorption of heterophile polyglycerophosphate antigen from antigen extracts of Streptococcus mutans and other gram-positive bacteria

Hamada, Shigeyuki; Tai, Stella; Slade, Hutton D.

doi:10.1128/iai.14.4.903-910.1976

Cited by 30 publications

(17 citation statements)

References 29 publications

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“…The S. mutans cell wall is known to contain PGP-type teichoic acids (9,19,24,51) in addition to the type-specific polysaccharides. It was of interest to determine whether these polymers function as binding sites for agglutination by Con A.…”

Section: Resultsmentioning

confidence: 99%

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Binding of lectins to Streptococcus mutans cells and type-specific polysaccharides, and effect on adherence

Hamada¹,

Gill²,

Slade³

1977

Infect Immun

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The lectin concanavalin A (Con A) agglutinated the cells of 13 of 15 strains of the seven serotypes of Streptococcus mutans in an 18-h incubation period. Strains of types a, d, f, and g agglutinated within 2 h. Strains of a, d, and f were also agglutinated in 2 h by the castor bean lectin RCA. S. sanguis, S. salivarius, S. bovis, Actinomyces viscosus, A. naeslundii, and Lactobacillus plantarum were agglutinated within 2 h. The S. mutans type f polysaccharide was precipitated by Con A. The a, b, c, d, and e polysaccharides were not precipitated. Glucan from d and e strains of S. mutans and dextran T2000 were also precipitated by Con A. D-glucose inhibited the agglutination of type f cells by Con A and the agglutination of type d cells by D-galactose. The quantity of [acetyl-3H]Con A bound was not proportional to the degree of agglutination. Cells grown in sucrose medium bound more Con A than those grown in glucose medium. After treatment with dextranase, the sucrose-grown cells bound twoto fourfold more Con A. The binding of Con A to the type-specific polysaccharide or to teichoic acid could not be determined by the use of specific antibody due to the binding of Con A to the antibody globulin on the cell surface. Con A bound to S. mutans cells did not inhibit the activity of cell-bound glucosyltransferase, glucan synthesis, and in vitro adherence. Bound Con A also did not inhibit the ability of heattreated cells to bind glucosyltransferase, synthesize glucan, and produce in vitro adherence. Streptococcus mutans, an organism that is associated with the development of dental caries in man and animals (43), has been classified into seven serotypes (a tof) (7,41). These type-specific antigens are polysaccharides located in the cell wall (15, 17, 22, 28, 30, 34, 35, 52). Recently plant lectins (agglutinins) have been found to react specifically with sugar residues at the nonreducing terminals of polysaccharides and glycoproteins (12, 31, 32, 45), frequently resulting in agglutination of bacterial cells (25, 26, 32, 44, 50). Among these lectins, concanavalin A (Con A) isolated from jack beans has been shown to react with polysaccharides containing aD -glycopyranosyl, aD -mannopyranosyl, or ,8-D-fructopyranosyl residues (13). On the other hand, an agglutinin (RCA) isolated from castor beans (Ricinus communis) was found to be specifically reactive with polysaccharide or glycoproteins containing a and /3-D-galactose residues (39, 40, 49). The reaction of Con A with teichoic acids has been reported by a number of workers (2, 5,8, 11, 24, 42).

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Section: Resultsmentioning

confidence: 99%

“…PGP antibodies from S. mutans whole cell antisera were removed by adsorption of the sera with group A streptococcal cells as previously described (19).…”

mentioning

confidence: 99%

Binding of lectins to Streptococcus mutans cells and type-specific polysaccharides, and effect on adherence

Hamada¹,

Gill²,

Slade³

1977

Infect Immun

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“…In the preparation of antisera to whole cells of S. mutans strain Ingbritt, one rabbit was found to respond specifically to PGP. This anti-PGP serum has been described previously (22). Anti-PGP serum reacts specifically with LTA in the PHA assay; and the serum yields one band on agar gel diffusion with LTA or with crude whole-cell extract containing glycerolteichoic acid.…”

Section: Materils and Methodsmentioning

confidence: 99%

Purification and characterization of a rhamnose-containing cell wall antigen of Streptococcus mutans B13 (serotype d)

Prakobphol¹,

Linzer²,

Genco³

1980

Infect Immun

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A rhamnose-containing polysaccharide (RCP) was extracted and purified from cell walls of Streptococcus mutans B13 (serotype d) and was chemically and immunologically characterized. Walls were initially extracted with 5% trichloroacetic acid at 4 degrees C to remove the serotype antigen and were then sequentially extracted with increasing concentrations of hot acid. Extracts lacking galactose were combined and chromatographed on a column of diethylaminoethyl--Sephadex A25. The purified RCP contained 90% carbohydrate, 1.4% protein, and 0.16% phosphorus. Analysis by gas chromatography indicated that the RCP was composed of rhamnose and glucose in a 1.6:1 ratio. RCP was immunogenic in rabbits when animals were immunized with whole cells or cell walls. Antisera prepared against partially extracted cell walls of B13 appeared specific for RCP. These sera were not reactive with purified serotype d antigen or lipoteichoic acid in passive hemagglutination assays or by agar gel diffusion. The RCP appeared to be a cell wall polysaccharide that was both chemically and immunologically distinct from the serotype d antigen.

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“…Cells (20 mg/ml of saline) were autoclaved at 120°C for 20 min. After centrifugation, the cell-free supernatant was employed as an antigen, as described previously (18).…”

Section: Preparation Of Antigen Reference and Clinicallymentioning

confidence: 99%

Isolation and immunobiological classification of Streptococcus sanguis from human tooth surfaces

Hamada¹,

Torii²,

Tsuchitani³

et al. 1980

J Clin Microbiol

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A total of 113 pure cultures of Streptococcus sanguis were obtained from dental plaque samples of 64 subjects. Ail isolates synthesized glucan from sucrose, elaborated peroxide, and were alpha-hemolytic. Two biotypes and four serotypes were differentiated within the species. Biotype A (95 isolates) fermented salicin and inulin and hydrolyzed arginine and esculin, whereas biotype B (18 isolates) did not possess these activities. The isolates were serotyped with autoclaved extracts against whole-cell antiserum to strains ATCC 10556 or ST3 (serotype I), ATCC 10557 (serotype II), ATCC 10558 (serotype III), and ST7 (serotype IV), by the capillary precipitin test. Serotypes I, II, III, and IV were found to consist of 24, 16, 37, and 15 isolates. Type IV was demonstrated anew in this study. The remaining 21 isolates were not typed because of either multiple reactions or nonreactivity against the standardized typing sera. Ail isolates of serotype Il belonged to biotype B, which resembles Streptococcus mitior physiologically. Five isolates representing four serotypes and an untypable strain were examined for their cariogenicity against specific-pathogen-free Sprague-Dawley rats fed high sucrose diet no. 2000. Organisms of each isolate were established in the mouths of the rats, but only three isolates induced weak caries that were restricted to pits and fissures of occlusal surfaces of the teeth.

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Selective adsorption of heterophile polyglycerophosphate antigen from antigen extracts of Streptococcus mutans and other gram-positive bacteria

Cited by 30 publications

References 29 publications

Binding of lectins to Streptococcus mutans cells and type-specific polysaccharides, and effect on adherence

Binding of lectins to Streptococcus mutans cells and type-specific polysaccharides, and effect on adherence

Purification and characterization of a rhamnose-containing cell wall antigen of Streptococcus mutans B13 (serotype d)

Isolation and immunobiological classification of Streptococcus sanguis from human tooth surfaces

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