Selective and rapid liquid chromatography/negative-ion electrospray ionization mass spectrometry method for the quantification of valacyclovir and its metabolite in human plasma

Analysis of the antiviral drugs acyclovir and valacyclovir-hydrochloride in tsetse flies (Glossina pallidipes) using LC–MSMS

“…The most abundant ion in both cases was the ion at m/z 152 (or 151.9), which possibly represents a protonated guanine species also reported elsewhere [12]. These ions possibly result from neutral loss of 2-amino-3-methyl butanoic ethylene ester (143 Da) and a formaldehyde (30 Da) following ␥-hydrogen rearrangement in the case of valacyclovir [13] and due to loss of a 2-methoxyethanol radical (74 Da) for acyclovir as proposed elsewhere [12]. We propose that the ions at m/z 135 in both compounds could result from loss of the NH 3 (or OH) radical from the protonated guanine (m/z 152 or 151.9).…”

Stability evaluation and sensitive determination of antiviral drug, valacyclovir and its metabolite acyclovir in human plasma by a rapid liquid chromatography–tandem mass spectrometry method

“…The lower limits of quantification were 250 and 200 ng/mL for VCV and ACV respectively and the chromatographic run time was 12 min. Recently, a selective and rapid liquid chromatography/negative-ion electrospray ionization mass spectrometry method has been reported for the quantification of VCV and its metabolite in human plasma [30]. The analytes were separated on a reversed-phase porous graphitized carbon column with a short analytical run time of 4 min and were monitored using SIM mode.…”

Development and validation of a sensitive LC-MS/MS method for determination of valacyclovir in human plasma: Application to a bioequivalence study

“…Several techniques such as high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods have been reported in the literature for the quantitative estimation of valacyclovir and acyclovir in biological fluids [2][3][4][5][6][7][8] and pharmaceutical dosage forms [9][10][11]. A number of methods were developed in animals such as rabbit [5], rat [6], and horse [7] plasma for quantification of valacyclovir and acyclovir by LC-MS/MS.…”

“…A number of methods were developed in animals such as rabbit [5], rat [6], and horse [7] plasma for quantification of valacyclovir and acyclovir by LC-MS/MS. Only a few methods were reported in human plasma for quantification of valacyclovir and acyclovir [2][3][4] by LC-MS/MS. Among all, Yadav M. and Upadhyav V. et al [2] achieved the best results.…”