Mycotoxins, with their teratogenic, carcinogenic, and mutagenic effects, pose a significant threat to food safety, socio-economic development, and human health. They are commonly found in cereal crops at concentrations that are difficult to detect, necessitating rapid, sensitive, and accurate detection methods. Metal-Organic Framework (MOF) boasts advantages such as a high specific surface area, high porosity, tunable structures, good conductivity, and stability, making them widely used as excellent metallic materials. Recently, many have made progress in constructing electrochemical sensors based on MOF in combination with metal nanoparticles, porphyrins, polycyclic aromatic hydrocarbons, graphene, etc., for the detection of mycotoxins in food, achieving promising results. In this review, we discuss the harmful effects of mycotoxins, list the regulatory standards for mycotoxins in food set by different organizations and countries, and highlight the methods for detecting common mycotoxins in food using MOF-based electrochemical sensors, providing a reference for subsequent detection of mycotoxins in food.