Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Meng, Chao; Zhao, Donglin; Guo, Wei; Chu, Yuanyuan; Chen, Guangping

doi:10.5772/63985

Cited by 12 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The linear regression equation was fitted as y = 0.1395x + 0.0875 with a correlation coefficient of 0.996 (n = 7). The limit of detection (LOD) was evaluated to 8.5 nM at 3s blank criterion, which was comparable with previously reported aptasensors, e.g., DNA/silver nanocluster-based fluorescence light-up system (91.6 nM) (Zhu et al, 2016), gold nanoparticles-based colorimetric assay (50 nM) (Huo et al, 2016), core-shell silver@SiO 2 nanoparticles-based fluorescent aptasensor (14.2 nM) (Song et al, 2016), AuNP-based fluorescence aptasensor (15.2 nM) (Meng et al, 2016) and Zinc(II)-protoporphyrin IX/G-quadruplex-based fluorescence aptasensor (10 μM) (Zhang et al, 2012). To further elucidate the merit of using the Tb-CDs for the development of fluorescence method, the AuNP-aptamer-NaCl system without Tb-CDs was also utilized for the detection of target ATP by using UV-vis absorption spectroscopy (Fig.…”

Section: Dose Responses Of the Fluorescence Aptasensing Platform Towasupporting

confidence: 88%

Terbium ion-coordinated carbon dots for fluorescent aptasensing of adenosine 5′-triphosphate with unmodified gold nanoparticles

Gao

Zhou

et al. 2016

Biosensors and Bioelectronics

View full text Add to dashboard Cite

This work reports on a novel time-resolved fluorescent aptasensing platform for the quantitative monitoring of adenosine 5'-triphosphate (ATP) by interaction of dispersive/agglomerate gold nanoparticles (AuNPs) with terbium ion-coordinated carbon dots (Tb-CDs). To construct such a fluorescent nanoprobe, Tb-CDs with high-efficient fluorescent intensity are first synthesized by the microwave method with terbium ions (Tb(3+)). The aptasensing system consists of ATP aptamer, AuNP and Tb-CD. The dispersive/agglomerate gold nanoparticles are acquired through the reaction of the aptamer with target ATP. Upon target ATP introduction, the aptamers bind with the analytes to form new aptamer-ATP complexes and coat on the surface of AuNPs to inhibit their aggregation in the high salt solution. In this case, the fluorescent signal of Tb-CDs is quenched by the dispersive AuNPs on the basis of the fluorescence resonance energy transfer (FRET). At the absence of target analyte, gold nanoparticles tend to aggregate in the high salt state even if the aptamers are present. Thus, the added Tb-CDs maintain their intrinsic fluorescent intensity. Experimental results indicated that the aptasensing system exhibited good fluorescent responses toward ATP in the dynamic range from 40nM to 4.0μM with a detection limit of 8.5nM at 3sblank criterion. The repeatability and intermediate precision is less than 9.5% at three concentrations including 0.04, 0.4 and 2.0μM ATP. The selectivity was acceptable toward guanosine 5'-triphosphate, uridine 5'-triphosphate and cytidine 5'-triphosphate. The methodology was applied to evaluate the blank human serum spiked with target ATP, and the recoveries (at 3 concentration levels) ranged between 97.0% and 103.7%. Importantly, this detection scheme is rapid, simple, cost-effective, and does not require extensive sample preparation or separation.

show abstract

Section: Dose Responses Of the Fluorescence Aptasensing Platform Towasupporting

confidence: 88%

Terbium ion-coordinated carbon dots for fluorescent aptasensing of adenosine 5′-triphosphate with unmodified gold nanoparticles

Gao

Zhou

et al. 2016

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…For example, in a lateral flow strip format, aptamer functionalized Gold-nanoparticles (AuNPs) have shown unequivocal visual detection of down to 2.5 nM thrombin (signal/noise = 3), providing superior sensitivity and specificity relative to conventional antibody-based assays (Xu et al, 2009 ). A FRET-based aptasensor was designed by Meng et al ( 2016 ) using functionalized AuNPs as energy acceptors; their assay showed no-cross reactivity to other nucleotides and could specifically detect ATP as low as 15.2 nmol/L.…”

Section: Enhancing Aptamer Assay Parametersmentioning

confidence: 99%

Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity

Kalra

Dhiman

Cho

et al. 2018

Front. Mol. Biosci.

126

View full text Add to dashboard Cite

Aptamers are structured nucleic acid molecules that can bind to their targets with high affinity and specificity. However, conventional SELEX (Systematic Evolution of Ligands by EXponential enrichment) methods may not necessarily produce aptamers of desired affinity and specificity. Thus, to address these questions, this perspective is intended to suggest some approaches and tips along with novel selection methods to enhance evolution of aptamers. This perspective covers latest novel innovations as well as a broad range of well-established approaches to improve the individual binding parameters (aptamer affinity, avidity, specificity and/or selectivity) of aptamers during and/or post-SELEX. The advantages and limitations of individual aptamer selection methods and post-SELEX optimizations, along with rational approaches to overcome these limitations are elucidated in each case. Further the impact of chosen selection milieus, linker-systems, aptamer cocktails and detection modules utilized in conjunction with target-specific aptamers, on the overall assay performance are discussed in detail, each with its own advantages and limitations. The simple variations suggested are easily available for facile implementation during and/or post-SELEX to develop ultrasensitive and specific assays. Finally, success studies of established aptamer-based assays are discussed, highlighting how they utilized some of the suggested methodologies to develop commercially successful point-of-care diagnostic assays.

show abstract

“…[17] Indeed, quite a few aptamer-based fluorescent sensors have been described. [8,16,[18][19][20][21] However, these sensors generally incorporate conventional dyes that are vulnerable to fluorescence quenching when they form aggregates at overly high concentration. This phenomenon, known as 'aggregationcaused quenching' (ACQ), is a notorious issue that restricts the usefulness of conventional dyes in biomolecular applications.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Biosensing with a Cationic AIEgen

et al. 2019

View full text Add to dashboard Cite

Fabrication of low-cost biosensing platforms with high selectivity and sensitivity is important for constructing portable devices for personal health monitoring. Herein, we report a simple biosensing strategy based on the combination of a cationic AIEgen (aggregation-induced emission fluorogen), TPE-2+, with an aptamer for specific protein detection. The target protein can displace the dye molecules on the dye–aptamer complex, resulting in changes in the fluorescence signal. Selectivity towards different targets can be achieved by simply changing the aptamer sequence. The working mechanism is also investigated.

show abstract

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Cited by 12 publications

References 27 publications

Terbium ion-coordinated carbon dots for fluorescent aptasensing of adenosine 5′-triphosphate with unmodified gold nanoparticles

Terbium ion-coordinated carbon dots for fluorescent aptasensing of adenosine 5′-triphosphate with unmodified gold nanoparticles

Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity

Aptamer-Based Biosensing with a Cationic AIEgen

Contact Info

Product

Resources

About