Selective antiproliferative activity of caffeic acid phenethyl ester analogues on highly liver-Metastatic murine colon 26-L5 carcinoma cell line

Merkl R., Hrádková I., Filip V., Šmidrkal J. (2010): Antimicrobial and antioxidant properties of phenolic acids alkyl esters. Czech J. Food Sci., 28: 275-279.Some phenolic acids alkyl esters (methyl, ethyl, propyl, butyl and hexyl) and determine their antioxidant and antimicrobial activities were prepared. The antimicrobial activity against the tested microorganisms escherichia coli DMF 7503, Bacillus cereus DMF 2001, listeria monocytogenes DMF 5776, Fusarium culmorum DMF 0103, and Saccharomyces cerevisiae DMF 1017 was investigated and expressed by minimum inhibitory concentration (MIC) in the range of 1.2-20mM. The inhibitory activity of phenolic acids butyl esters was found to be higher than that of methyl esters (MIC below 1.25mM). The antioxidant activity of the selected phenolic acids alkyl esters was investigated by Rancimat method. The esters of 3,4-dihydroxyphenolic acids (protocatechuic and caffeic acids) exhibited higher antioxidant activities in comparison with the respective phenolic acids. The highest antioxidant activity was found in the case of caffeic alkyl esters.

show abstract

“…The mobile phase was chloroform/methanol (9:1). The spots were visualised under UV (254 nm) (Nagaoka et al 2002).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and antioxidant properties of phenolic acids alkyl esters

Merkl¹,

Hrádková²,

Filip³

et al. 2010

Czech J. Food Sci.

228

142

View full text Add to dashboard Cite

show abstract

“…Thus, it was considered that inhibiting STAT3 phosphorylation at Tyr705 might reduce VEGF expression in tumor cells. CA and its synthetic derivative, CADPE [3-(3,4-Dihydroxyphenyl)-acrylic acid 2-(3,4-dihydroxy-phenyl)-ethyl ester] were found to be actively involved in inhibiting STAT3 activity [219,220]. Since STAT3 phosphorylation at Tyr705 is essential for its nuclear translocation as well as for binding to specific promoter sequences on its target genes, the activities of CA and CADPE were investigated against STAT3 phosphorylation in vitro, using renal carcinoma Caki cells.…”

Section: Caffeic Acidmentioning

confidence: 99%

Targeting the STAT3 signaling pathway in cancer: Role of synthetic and natural inhibitors

Siveen

Sikka

Surana

et al. 2014

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

481

510

View full text Add to dashboard Cite

Signal transducers and activators of transcription (STATs) comprise a family of cytoplasmic transcription factors that mediate intracellular signaling that is usually generated at cell surface receptors and thereby transmit it to the nucleus. Numerous studies have demonstrated constitutive activation of STAT3 in a wide variety of human tumors, including hematological malignancies (leukemias, lymphomas, and multiple myeloma) as well as diverse solid tumors (such as head and neck, breast, lung, gastric, hepatocellular, colorectal and prostate cancers). There is strong evidence to suggest that aberrant STAT3 signaling promotes initiation and progression of human cancers by either inhibiting apoptosis or inducing cell proliferation, angiogenesis, invasion, and metastasis. Suppression of STAT3 activation results in the induction of apoptosis in tumor cells, and accordingly its pharmacological modulation by tyrosine kinase inhibitors, antisense oligonucleotides, decoy nucleotides, dominant negative proteins, RNA interference and chemopreventive agents have been employed to suppress the proliferation of various human cancer cells in culture and tumorigenicity in vivo. However, the identification and development of novel drugs that can target deregulated STAT3 activation effectively remains an important scientific and clinical challenge. This review presents the evidence for critical roles of STAT3 in oncogenesis and discusses the potential for development of novel cancer therapies based on mechanistic understanding of STAT3 signaling cascade.

show abstract

“…(Marder et al, 1998). These molecules have been reported to possess tremendous pharmacological properties, which include antimicrobial, antioxidant and anticancer activities (Nagaoka et al, 2002). The bioactivities of flavonoids are attributed to their ability to interact with a diversity of cellular enzymes.…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of 5, 7-Dimethoxyflavone Against Liver Cancer Cell Line Hepg2 Involves Apoptosis, Ros Generation and Cell Cycle Arrest

Zhang

Wang

2017

Afr J Tradit Complement Altern Med

View full text Add to dashboard Cite

Background: Flavonoids are considered potential anticancer agents owing to their properties to interact with a diversity of cellular entities. Among flavonoids, methylated flavones are more efficient anticancer agents due to their higher stability in vivo. The purpose of the present study was, therefore, to evaluate the anticancer effect of methylated natural flavonoid 5, 7-dimethoxyflavone (5, 7-DMF) Materials and methods: MTT assay was used to determine the anticancer activity and IC50 of 5, -DMF). Cell viability, cell cycle distribution, reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) were carried out by flow cytometry. Apoptosis was studied by DAPI staining. Results: MTT assay revealed that the molecule reduced the cell viability of HepG2 cancer cells. The IC50 of 5, 7-DMF was found to be 25 µM. Our result indicated that 5, 7-DMF triggered production of ROS and significantly reduced ΔΨm. It also leads to arrest of HepG2 cells in Sub-G1 stage of cell cycle, and ultimately induced apoptosis in a concentration-dependent manner, as indicated by DAPI staging. Additionally, 5, 7-DMF also reduced the colony forming potential of the HepG2 cells concentration dependently. Conclusion: Taken together, we conclude that 5, 7-DMF induces cell death via ROS generation, cell cycle arrest and apoptosis, and, therefore, may prove beneficial in the treatment of liver cancer.

show abstract

Selective antiproliferative activity of caffeic acid phenethyl ester analogues on highly liver-Metastatic murine colon 26-L5 carcinoma cell line

Cited by 122 publications

References 18 publications

Antimicrobial and antioxidant properties of phenolic acids alkyl esters

Antimicrobial and antioxidant properties of phenolic acids alkyl esters

Targeting the STAT3 signaling pathway in cancer: Role of synthetic and natural inhibitors

Anticancer Activity of 5, 7-Dimethoxyflavone Against Liver Cancer Cell Line Hepg2 Involves Apoptosis, Ros Generation and Cell Cycle Arrest

Contact Info

Product

Resources

About