Selective axonal transport through branch junctions is directed by growth cone signaling and mediated by KIF1/kinesin-3 motors

Tymanskyj, Stephen R.; Curran, Bridget Mary; Ma, Le

doi:10.1016/j.celrep.2022.110748

Cited by 8 publications

(1 citation statement)

References 60 publications

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“…Since branches formed from a single neuron can be heterogeneous in synapse formation and activity, it is reasonable to conclude that these branches may also differ from each other in the distribution of synaptic cargo. A recent study (Tymanskyj, Curran, and Ma, 2022) demonstrates that triggering of growth cone activity specifically at the end of one axonal branch of vertebrate neurons in culture leads to preferential transport of synaptic vesicles and LAMP1 vesicles into that branch. It is interesting to investigate whether axonal branches exhibit heterogeneous distribution of synaptic cargo in neurons in vivo, and the underlying mechanisms that bring this about.…”

Section: Introductionmentioning

confidence: 99%

Preferential transport of synaptic vesicles across neuronal branches is regulated by the levels of the anterograde motor UNC-104/KIF1A

Vasudevan

Ratnakaran

Murthy

et al. 2023

Preprint

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Asymmetric transport of cargo across axonal branches is a field of active research. Mechanisms contributing to preferential cargo transport along specific branchesin vivoin wild type neurons are poorly understood. We find that anterograde synaptic vesicles preferentially enter the synaptic branch or pause at the branch point inC. elegansPLM neurons. The anterograde motor UNC-104/KIF1A regulates this vesicle behaviour at the branch point. Reduced levels of functional UNC-104 cause vesicles to predominantly pause at the branch point and lose their preference for turning into the synaptic branch. SAM-4/Myrlysin, which aids in recruitment/activation of UNC-104 on synaptic vesicles, regulates vesicle behaviour at the branch point similar to UNC-104. Increasing the levels of UNC-104 increases the preference of vesicles to go straight towards the asynaptic end. This suggests that the neuron optimises UNC-104 levels on the cargo surface to maximise the fraction of vesicles entering the branch and minimise the fraction going to the asynaptic end.

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Section: Introductionmentioning

confidence: 99%