Selective binding, magnetic separation and purification of histidine-tagged protein using biopolymer magnetic core-shell nanoparticles

Zhou, Yang; Yan, Dandan; Yuan, Shaofei; Chen, Yawen; Fletcher, Emmanuella E.; Shi, Haifeng; Han, Bangxing

doi:10.1016/j.pep.2017.11.004

Cited by 37 publications

(25 citation statements)

References 31 publications

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“…Exclusion of HMW proteins with the Ni 2+ -IDA-CSS-NP is more evident compared to other reports of IMAC-based coreshell sorbents. 10,40 In our work, HMW proteins were not observed, even with a more sensitive staining protocol (silver staining), whereas in other studies 10,40 HMW proteins were abundant, as they were observed using a less sensitive staining protocol such as Coomassie staining. 41 Adsorption capacity of Ni 2+ -IDA-CSS-NP was determined by estimating the amount of protein in the elutions by Bradford assay 21 from three different purications, and resulted in 4.16 AE 0.45 mg mg À1 of sorbent and this capacity was higher than that reported by Li and colleagues in 2016, 42 where they used modied Ni 2+ -IDA magnetic silica nanoparticles and presented a capacity of 3.6 mg mg À1 of sorbent.…”

Section: His-spo0f Recombinant Protein Purication By the Ni 2+ -Idacontrasting

confidence: 64%

Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

et al. 2019

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Section: His-spo0f Recombinant Protein Purication By the Ni 2+ -Idacontrasting

confidence: 64%

Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

et al. 2019

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“…It was first discovered that a couple of nanoparticles almost like the following metals, MNP, ZnO, TiO 2 , CuO, and Ag 2 O is having a tendency to precipitate the proteins and confirmed DNA with great quality [4,24]. Magnetic nanoparticles offer an elevating multitude of a common approach for the purification of biomolecules, and DNA isolation from bacteria and fungi respectively [25].…”

Section: Discussionmentioning

confidence: 99%

Magnetic-silica nanoshell for extraction of fungal genomic DNA from Rhizopus oryzae

2020

Biointerface Res Appl Chem

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The main objective of the present article describes an established genomic DNA purification technique improved in vitro by using magnetic nanoparticles as a stable phase adsorbent. Stöber technique was used to synthesis Fe3O4@SiO3 nanoshell, and described using transmission electron microscopy (TEM) and dynamic light scattering. The quality and quantity of purified DNA were verified by using agarose gel electrophoresis and some PCR strategies such as universal-primed UP-PCR, RAPD patterns. Up to 50 µg of DNA was obtained from a slight quantity of one hundred mg of fungal mycelium. Fungal DNA produced with the aid of this technique was applied as a fungal DNA template for PCR method to amplify UP-PCR, RAPD provided replicable profiles. DNA purified from fungal mats was 100 % pure enough to generally be used at high concentrations for PCR technique. The end result confirmed that Fe3O 4 @SiO 3 nanoparticles of ∼140 nm diameter with concentration of 0.4 mg/ml produced premium quality and amount of isolated DNA produced from fungal mats. The obtained DNA was free from any polluting proteins, polysaccharides and coloured dyes. Magnetic nanoparticles (MNP)-mediated genomic DNA extraction is pretty simple (loose from filtration and centrifugation), speedy (30 min), and ecofriendly (not contain any toxic chemical compounds).

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“…One way to overcome this hurdle is to use a combined series of extraction/purification procedures, which does, however, conflict with the aim of realizing a simple, rapid on-site and low-cost sample-processing device for biomarker monitoring. As for magnetic separation, Figure 18 shows a time-efficient and relatively simplistic way to concentrate specific protein analytes from a complex matrix [142]. This technique can be applied to the purification of proteins, cells, or other biomolecules [143].…”

Section: How To Detect On-sitementioning

confidence: 99%

“…A simplified example of magnetic protein separation using functionalized-magnetic beads in a single tube. (Re-printed from Zhou et al[142]. Copyright (2018), with permission from Elsevier).…”

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confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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Selective binding, magnetic separation and purification of histidine-tagged protein using biopolymer magnetic core-shell nanoparticles

Cited by 37 publications

References 31 publications

Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

Magnetic-silica nanoshell for extraction of fungal genomic DNA from Rhizopus oryzae

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

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