Selective Biosynthesis of Furoic Acid From Furfural by Pseudomonas Putida and Identification of Molybdate Transporter Involvement in Furfural Oxidation

Zheng, Zhaojuan; Xu, Qianqian; Tan, Huanghong; Zhou, Feng; Ouyang, Jia

doi:10.3389/fchem.2020.587456

Cited by 19 publications

(15 citation statements)

References 49 publications

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“…It should be noted that the ped cluster encoding the above-mentioned dehydrogenases is not well conserved among Pseudomonads, and strains like P. taiwanensis VLB120 completely lack the cluster (pseudomonas.com [94]). However, Pseudomonads have a large repertoire of other dehydrogenases, and recent studies point to the involvement of, e.g., molybdenum-dependent enzymes [95][96][97].…”

Section: Dealing With the Toxicant: Detoxification And Damage Recoverymentioning

confidence: 99%

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

Bitzenhofer

Kruse

Thies

et al. 2021

Essays in Biochemistry

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Biotechnological production in bacteria enables access to numerous valuable chemical compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well as the combinatorial use of biocatalysts, pathways, and circuits even bring new-to-nature compounds within reach. However, the associated substrates and biosynthetic products often cause severe chemical stress to the bacterial hosts. Species of the Pseudomonas clade thus represent especially valuable chassis as they are endowed with multiple stress response mechanisms, which allow them to cope with a variety of harmful chemicals. A built-in cell envelope stress response enables fast adaptations that sustain membrane integrity under adverse conditions. Further, effective export machineries can prevent intracellular accumulation of diverse harmful compounds. Finally, toxic chemicals such as reactive aldehydes can be eliminated by oxidation and stress-induced damage can be recovered. Exploiting and engineering these features will be essential to support an effective production of natural compounds and new chemicals. In this article, we therefore discuss major resistance strategies of Pseudomonads along with approaches pursued for their targeted exploitation and engineering in a biotechnological context. We further highlight strategies for the identification of yet unknown tolerance-associated genes and their utilisation for engineering next-generation chassis and finally discuss effective measures for pathway fine-tuning to establish stable cell factories for the effective production of natural compounds and novel biochemicals.

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Section: Dealing With the Toxicant: Detoxification And Damage Recoverymentioning

confidence: 99%

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

Bitzenhofer

Kruse

Thies

et al. 2021

Essays in Biochemistry

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“…The construction of gcd and gtsABCD mutants was conducted as described in our previous study [ 43 ]. Specifically, genomic DNA of P. putida KT2440 was isolated using a Bacteria Genomic DNA Extraction Kit (Takara, Japan).…”

Section: Methodsmentioning

confidence: 99%

Efficient lactic acid production from dilute acid-pretreated lignocellulosic biomass by a synthetic consortium of engineered Pseudomonas putida and Bacillus coagulans

Zou

Ouyang

et al. 2021

Biotechnol Biofuels

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Background Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production of a range of biochemicals, and pretreatment is generally regarded as indispensable for its biorefinery. However, various inhibitors that severely hinder the growth and fermentation of microorganisms are inevitably produced during the pretreatment of lignocellulose. Presently, there are few reports on a single microorganism that can detoxify or tolerate toxic mixtures of pretreated lignocellulose hydrolysate while effectively transforming sugar components into valuable compounds. Alternatively, microbial coculture provides a simpler and more efficacious way to realize this goal by distributing metabolic functions among different specialized strains. Results In this study, a novel synthetic microbial consortium, which is composed of a responsible for detoxification bacterium engineered Pseudomonas putida KT2440 and a lactic acid production specialist Bacillus coagulans NL01, was developed to directly produce lactic acid from highly toxic lignocellulosic hydrolysate. The engineered P. putida with deletion of the sugar metabolism pathway was unable to consume the major fermentable sugars of lignocellulosic hydrolysate but exhibited great tolerance to 10 g/L sodium acetate, 5 g/L levulinic acid, 10 mM furfural and HMF as well as 2 g/L monophenol compound. In addition, the engineered strain rapidly removed diverse inhibitors of real hydrolysate. The degradation rate of organic acids (acetate, levulinic acid) and the conversion rate of furan aldehyde were both 100%, and the removal rate of most monoaromatic compounds remained at approximately 90%. With detoxification using engineered P. putida for 24 h, the 30% (v/v) hydrolysate was fermented to 35.8 g/L lactic acid by B. coagulans with a lactic acid yield of 0.8 g/g total sugars. Compared with that of the single culture of B. coagulans without lactic acid production, the fermentation performance of microbial coculture was significantly improved. Conclusions The microbial coculture system constructed in this study demonstrated the strong potential of the process for the biosynthesis of valuable products from lignocellulosic hydrolysates containing high concentrations of complex inhibitors by specifically recruiting consortia of robust microorganisms with desirable characteristics and also provided a feasible and attractive method for the bioconversion of lignocellulosic biomass to other value-added biochemicals.

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“…102 Ouyang and co-workers evaluated the ability of P. putida KT2440 (also deposited as DSM 6125 and ATCC 47054) to oxidize HMF and furfural. 103,104 This strain was able to smoothly convert around 2, entry 13). The authors identified a Mo-containing transporter from this microorganism that is involved in the oxidation of the furans.…”

Section: Whole-cell Catalysismentioning

confidence: 97%

“…P. putida is an important industrial microorganism because of its versatile metabolism and low nutritional requirements as well as the high robustness against extreme environmental conditions such as extreme pH and tolerance to toxic chemicals and solvents . Ouyang and co-workers evaluated the ability of P. putida KT2440 (also deposited as DSM 6125 and ATCC 47054) to oxidize HMF and furfural. , This strain was able to smoothly convert around 170 mM of furans to the target carboxylic acids, with almost quantitative yields (Table , entry 13). The authors identified a Mo-containing transporter from this microorganism that is involved in the oxidation of the furans .…”

Section: Catalytic Oxidationmentioning

confidence: 99%

“…Ouyang and co-workers evaluated the ability of P. putida KT2440 (also deposited as DSM 6125 and ATCC 47054) to oxidize HMF and furfural. , This strain was able to smoothly convert around 170 mM of furans to the target carboxylic acids, with almost quantitative yields (Table , entry 13). The authors identified a Mo-containing transporter from this microorganism that is involved in the oxidation of the furans . A radiation-resistant strain Deinococcus wulumuqiensis R12 was reported to be highly HMF-tolerant, enabling effective oxidation of 300 mM HMF to HMFCA (90% yield) .…”

Section: Catalytic Oxidationmentioning

confidence: 99%

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(Chemo)biocatalytic Upgrading of Biobased Furanic Platforms to Chemicals, Fuels, and Materials: A Comprehensive Review

Zong

2022

ACS Catal.

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Biobased furans, such as furfural, 5-hydroxymethylfurfural, and 2,5-furandicarboxylic acid, are recognized as the top-value platform chemicals in the valorization of biomass to chemicals, fuels, and materials. Biocatalysis has emerged as a promising technique in the chemical and pharmaceutical industries because of exquisite selectivity, mild reaction conditions, environmental friendliness, etc. In this comprehensive review, we summarize the recent advances in (chemo)biocatalytic conversion of biobased furans, based on the reaction types including oxidation, reduction, C–C and C–N bonds formation, esterification, and amidation. Particularly, the biocatalysts and their catalytic mechanisms/pathways are critically discussed to allow the rational design and construction of efficient enzymes/multienzyme systems/chemobiocatalytic systems. Besides, various biocatalyst engineering and reaction engineering strategies are highlighted to intensify the processes. Future research directions are proposed to expand the chemical space of furans as well as to improve the commercial acceptance of biocatalytic processes.

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Selective Biosynthesis of Furoic Acid From Furfural by Pseudomonas Putida and Identification of Molybdate Transporter Involvement in Furfural Oxidation

Cited by 19 publications

References 49 publications

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

Efficient lactic acid production from dilute acid-pretreated lignocellulosic biomass by a synthetic consortium of engineered Pseudomonas putida and Bacillus coagulans

(Chemo)biocatalytic Upgrading of Biobased Furanic Platforms to Chemicals, Fuels, and Materials: A Comprehensive Review

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