Selective capture and rapid identification of Panax notoginseng metabolites in rat faeces by the integration of magnetic molecularly imprinted polymers and high-performance liquid chromatography coupled with orbitrap mass spectrometry

Cai, Qizhi; Yang, Zhaochu; Chen, Ning; Zhou, Xuemin; Hong, Junli

doi:10.1016/j.chroma.2016.05.089

Cited by 21 publications

(10 citation statements)

References 40 publications

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“…Fe 3 O 4 magnetic nanoparticles were prepared based on the method reported in the literature . Subsequently, Fe 3 O 4 (0.3 g) were dispersed into ethanol (50 mL), ammonium hydroxide (2 mL), and ultra‐pure water (2 mL) were added sequentially, TEOS (5 mL) was added slowly, and then the mixture was kept under stirring at room temperature for 12 h. The synthesized SiO 2 @Fe 3 O 4 was separated, washed with ultra‐pure water, and dried in a vacuum.…”

Section: Methodsmentioning

confidence: 99%

“…However, there are certain drawbacks that exist such as long adsorption time, low binding capacity and selectivity, irregular shape and poor sites accessible due to the crushed polymers and incomplete template removal [31][32][33][34][35]. To overcome these limitations, surface imprinting has been developed in recent years [35][36][37][38][39][40]. Surface imprinting often makes use of silica gel particles due to their large surface area and low cost.…”

Section: Introductionmentioning

confidence: 99%

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Efficient discovery and capture of new neuronal nitric oxide synthase–postsynaptic density protein‐95 uncouplers from herbal medicines using magnetic molecularly imprinted polymers as artificial antibodies

Huang

Zhang

et al. 2017

J of Separation Science

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In the scope of stroke treatment, new neuronal nitric oxide synthase-postsynaptic density protein-95 uncouplers from herbal medicines were discovered and captured. To do so, highly selective magnetic molecularly imprinted polymers with a core-shell structure were prepared as artificial antibodies. According to the results of computational simulations, we designed and synthesized various polymers with varying amounts and types of template, functional monomer, cross-linker, and solvent. Characterization and performance tests revealed that the most appropriate artificial antibodies showed uniform spherical morphologies, large adsorption capacities, fast-binding kinetics, high selectivity, and quick separation. These artificial antibodies were then used as sorbents for dispersive magnetic solid-phase extraction coupled with high-performance liquid chromatography and mass spectrometry to capture and identify structural analogs to ZL006 from extracts of Scutellariae radix, Psoraleae fructus, and Trifolium pratense. Furthermore, according to the neuroprotective effect and coimmunoprecipitation test, Baicalein, Neobavaisoflavone, Corylifol A, and Biochanin A can be the potential uncouplers of neuronal nitric oxide synthase-postsynaptic density protein-95. Therefore, this present study contributes valuable information for the discovery of neuronal nitric oxide synthase-postsynaptic density protein-95 uncouplers from herbal medicines.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient discovery and capture of new neuronal nitric oxide synthase–postsynaptic density protein‐95 uncouplers from herbal medicines using magnetic molecularly imprinted polymers as artificial antibodies

Huang

Zhang

et al. 2017

J of Separation Science

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“…In the past few decades, MIPs have found important and promising applications such as separation [23–25], sensing [26–28], disease diagnosis [29–31], toxicity neutralization [32], and nanomedicine [33]. However, the applications of MIPs to saponins still remains challenging and limited [34,35]. This could be attributed to the amphiphilicity of saponins.…”

Section: Introductionmentioning

confidence: 99%

Preparing molecularly imprinted nanoparticles of saponins via cooperative imprinting strategy

Zhang

Wang

et al. 2020

J of Separation Science

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Saponin is an important class of natural products with various pharmacological activities. The selective separation of saponins is an essential step before further analysis.Molecular imprinting has been an effective strategy for preparing antibody mimics. However, a facile and efficient imprinting strategy for saponins is still lacking owing to their amphiphilic nature. Herein, we have prepared the saponins imprinted nanoparticles via cooperative imprinting strategy. This new strategy relies on the combination of various non-covalent interactions (hydrophobic and hydrogen bonding) and covalent boronate affinity interactions. The obtained imprinted nanoparticles could rebind specific saponins from complex matrices with good selectivity, superb tolerance to interference, and fast binding equilibrium. This method was verified to be versatile and facile. Thus, this strategy could greatly facilitate the preparation of imprinted nanoparticles for the specific recognition of saponins. K E Y W O R D Samphiphilic molecules, mass spectrometry, molecular imprinting, sample preparation, saponins 2162

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“…LC is the most prevalent separation technique for mixtures of ginsenosides because of its speed and selectivity. LC has the additional advantage of compatibility with various detection techniques ______________________________________________________________________________________________________ such as UV absorbance [15][16][17][18][19], evaporative light scattering (ELS) [20], fluorescence (FL) [21], pulsed amperometry (PA) [22], MS [15,23,24], and tandem mass spectrometry (MS/MS) [25][26][27]. UV is the most frequently used detector for simple sample matrixes because of its inexpensive cost and simplicity, but ginsenoside detection is limited to short wavelengths of 200 nm to 205 nm.…”

Section: Introductionmentioning

confidence: 99%

Certification of standard reference material 3389 ginsenoside calibration solution

Wilson¹,

Sander²,

Place³

et al. 2019

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Standard Reference Material (SRM) 3389 is intended as a calibration solution for use in the determination of ginsenosides in natural matrix samples. A unit of SRM 3389 consists of two different ampule solutions: (1) four ampules of a six-component mixture of ginsenoside Rb1, Rb2, Rc, Rd, Re, and Rg1 and (2) one ampule of a single component ginsenoside Rf. This publication documents the production, analytical methods, and statistical evaluations involved in production of this SRM.

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Selective capture and rapid identification of Panax notoginseng metabolites in rat faeces by the integration of magnetic molecularly imprinted polymers and high-performance liquid chromatography coupled with orbitrap mass spectrometry

Cited by 21 publications

References 40 publications

Efficient discovery and capture of new neuronal nitric oxide synthase–postsynaptic density protein‐95 uncouplers from herbal medicines using magnetic molecularly imprinted polymers as artificial antibodies

Efficient discovery and capture of new neuronal nitric oxide synthase–postsynaptic density protein‐95 uncouplers from herbal medicines using magnetic molecularly imprinted polymers as artificial antibodies

Preparing molecularly imprinted nanoparticles of saponins via cooperative imprinting strategy

Certification of standard reference material 3389 ginsenoside calibration solution

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