Selective cell encapsulation, lysis, pico-injection and size-controlled droplet generation using traveling surface acoustic waves in a microfluidic device

Mutafopulos, Kirk; Lu, Peter J.; Garry, Ryan; Spink, Pascal; Weitz, David A.

doi:10.1039/d0lc00723d

Cited by 34 publications

(30 citation statements)

References 45 publications

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“…Double emulsions (DE) droplets overcome the aforementioned problems since the inner phase and the external environment are isolated through a core-shell structure ( Hou et al, 2017 ; Wu et al, 2020 ; Jeong et al, 2021 ), which is formed by having one type of droplet dispersed in a lager immiscible droplet. As both the inner core and the shell structure can be separately controlled, DE droplets have been proven to be very useful for single-cell analysis ( Moore et al, 2018 ; Zhang et al, 2018 ; Mutafopulos et al, 2020 ), transport of active and ions ( Xie et al, 2017 ; Chowdhury et al, 2019 ; Pei et al, 2019 ; Sun et al, 2020 ; Wu et al, 2020 ; Jeong et al, 2021 ), functional microparticles ( Kim et al, 2014 ), and particularly, fabrication of microcapsules through the gelation of the shell structure.…”

Section: Introductionmentioning

confidence: 99%

Simple One-Step and Rapid Patterning of PDMS Microfluidic Device Wettability for PDMS Shell Production

Feng

Takahashi

Zhu

2022

Front. Bioeng. Biotechnol.

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Double emulsion (DE) droplets with controlled size and internal structure are a promising platform for biological analysis, chemical synthesis, and drug delivery systems. However, to further “democratize” their application, new methods that enable simple and precise spatial patterning of the surface wettability of droplet-generating microfluidic devices are still needed. Here, by leveraging the increase in hydrophilicity of polydimethylsiloxane (PDMS) due to the plasma-treatment used to permanently bond to glass, we developed a one-step method to selectively pattern the wettability of PDMS microfluidic devices for DE generation. Our results show that both Aquapel-treated and 1H,1H,2H,2H-Perfluorodecyltriethoxysilan (PFDTES)-treated devices are functionally showing the generality of our method. With the resulting microfluidic devices, both water-in-oil-in-water (w/o/w) and oil-in-water-in-oil (o/w/o) DE droplets can be produced. Using a PDMS mixture containing cross-linking agents, we formed PDMS microcapsules by solidifying the shell layer of water-in-PDMS-in-water DE droplets. We also characterize the morphological properties of the generated droplets/microcapsules. We anticipate the method developed in this work could be used in a broad range of applications of DE droplets.

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Section: Introductionmentioning

confidence: 99%

Simple One-Step and Rapid Patterning of PDMS Microfluidic Device Wettability for PDMS Shell Production

Feng

Takahashi

Zhu

2022

Front. Bioeng. Biotechnol.

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“…Two types of IDT manufacturing approaches have been used to date: (1) patterning IDTs directly onto piezoelectric substrates exposed to air, commonly lithium niobate wafers; or (2) embedding conductive metal liquids into channels shaped in the form of IDTs within PDMSbased microfluidic devices constructed on a piezoelectric substrate. SAW-producing microfluidic units with patterned IDTs open to the air have already been established for several droplet-based microfluidics applications such as droplet merging [19,21,22], production [23,24], injection [24], splitting [25] and sorting [26,27]. In contrast, embedded IDTs have so far been implemented for droplet production [28] and micro-mixing within a single aqueous droplet [29,30].…”

Section: Introductionmentioning

confidence: 99%

“…For example, many systems that operate in the MHz frequency domain restrict exposure time of biological samples to the acoustic field [35] or implement complicated feedback temperature control strategies including Peltier stages [36] to mitigate thermal contributions. Other works attempt to exploit device heating by using the phenomena in applications such as continuous flow polymerase chain reactions [20] or to deliberately kill cells [24]. A simple, integrated droplet-based acoustofluidics device that is mechanistically characterized and acoustically manipulates droplets with physiologically relevant conditions has yet to be shown.…”

Section: Introductionmentioning

confidence: 99%

Controlled microfluidic droplet acoustoinjection on one chip

Frey

Lora

Jahnke

et al. 2022

Preprint

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We present an all-in-one acoustofluidics device for controlled acoustic field-mediated injection of surfactant stabilized water-in-oil droplets. The microfluidic channels and interdigitated transducer (IDT) channels are produced on the same master wafer and cast within one PDMS slab, making our acoustofluidics device simple to construct while retaining the same height for all channels. The IDTs with a curved, serpentine, paired and focusing geometry are easily embedded into the PDMS slab by filling the IDT channels with low melting point metal alloy. In this article, we propose the working mechanism of our embedded IDTs, which we call acoustoinjection, and carry out a precise characterization by laser doppler vibrometry (LDV) and infrared imaging to describe the injection of droplets within microfluidic channels. Although we observe that the device has acoustic resonance in the MHz frequency domain, we show that it operates most efficiently for acoustoinjection in the kHz frequency domain. In this frequency domain, our acoustofluidics device generates a pressure wave that causes destabilization of the surfactant-supported droplet interface enabling the injection of aqueous solution into the water-phase of the droplet with minimum heat generation. We show droplet injection for different surfactant concentrations, droplet passing speeds, and injection rates with high accuracy. This integrated device has the potential to serve as an alternative to electric field mediated picoinjection technologies by acoustic field-mediated and non-harmful manipulation of droplets with bio-content.

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“…Bright-field imaging is capable of directly measuring the absorption of cells and has been applied to protein depiction in red blood cells or stained cells. 6,7 However, it suffers from insufficient imaging contrast and volumetric resolving capability. Apart from amplitude imaging, phase imaging significantly amplifies the imaging contrast, 8−12 whereas, encounters difficulties in identifying and sorting cells due to the lack of imaging specificity.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The application of flow cytometry enables single-cell imaging, provides detail cellular information, and works as a prevalent tool for sorting, capture, and elimination of specific cells, making it accessible to various applications such as cell observation, − analysis, and manipulation. , Among the methodologies, optical microscopy, featuring nonionization, rich image contrast, and high spatiotemporal resolution, remains one of the fastest evolving microscopies utilized in cell imaging applications. Bright-field imaging is capable of directly measuring the absorption of cells and has been applied to protein depiction in red blood cells or stained cells. , However, it suffers from insufficient imaging contrast and volumetric resolving capability. Apart from amplitude imaging, phase imaging significantly amplifies the imaging contrast, − whereas, encounters difficulties in identifying and sorting cells due to the lack of imaging specificity.…”

Section: Introductionmentioning

confidence: 99%

Acoustic Standing Wave Aided Multiparametric Photoacoustic Imaging Flow Cytometry

Sun

Jin

et al. 2021

Anal. Chem.

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Photoacoustic imaging reveals great potential for the study of individual cells due to the rich imaging contrast for both label-free and labeled cells. However, previously reported photoacoustic imaging flow cytometry configuration suffers from inadequate imaging quality and challenge to distinguish multiple cells. In order to solve such issues, we propose a novel acoustic standing wave aided multiparametric photoacoustic imaging flow cytometry (MPAFC) system. The acoustic standing wave is introduced to improve the imaging quality and speed. Multispectral illumination along with cell geometry, photoacoustic amplitude, and acoustic frequency spectrum enables the proposed system to precisely identify multiple types of cells with one scanning. On the basis of the identification, elimination of melanoma cells, and targeted labeled glioma cells have been performed with an elimination efficiency of >95%. Additionally, the MPAFC system is able to image and capture melanoma cells at a lowest concentration of 100 cells mL −1 in pure blood. Current results suggest that the proposed MPAFC may provide a precise and efficient tool for cell detection, manipulation, and elimination in both fundamental and clinical studies.

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Selective cell encapsulation, lysis, pico-injection and size-controlled droplet generation using traveling surface acoustic waves in a microfluidic device

Abstract: We generate traveling surface acoustic waves with an interdigital transducer to create droplets on-demand; encapsulate single cells; lyse cells and immediately encapsulate their contents; and pico-inject new materials into existing droplets.

Cited by 34 publications

References 45 publications

Simple One-Step and Rapid Patterning of PDMS Microfluidic Device Wettability for PDMS Shell Production

Simple One-Step and Rapid Patterning of PDMS Microfluidic Device Wettability for PDMS Shell Production

Controlled microfluidic droplet acoustoinjection on one chip

Acoustic Standing Wave Aided Multiparametric Photoacoustic Imaging Flow Cytometry

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