Selective cell killing by microparticle absorption of pulsed laser radiation

Lin, Charles P.; Kelly, Michael; Sibayan, Santiago Antonio B; Latina, Mark A.; Anderson, R. Rox

doi:10.1109/2944.796318

Cited by 104 publications

(96 citation statements)

References 11 publications

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“…This is in agreement with Kelly et al who showed that cell death coincided with intracellular bubble formation detected by the stroboscopic imaging method [1][2][3]. Although the thresholds were the same (on average), some false positive and some false negative events were observed (6 false positive and 7 false negative events were detected out of 77 cells within 20% of the ED50 threshold).…”

Section: Significancesupporting

confidence: 79%

“…As the rate of energy deposition exceeds the rate of thermal relaxation, the energy becomes confined to the absorbing structuresthe melanin granules -within the RPE layer. These pigment microparticles form intracellular hot spots and initiate microscopic cavitation bubbles that expand and collapse on the timescale of 0.1 -1 ^sec [1][2][3]. The mechanical actions associated with microbubble expansion and implosion imparts damage to cells [2][3][4][5].…”

Section: Collaborators/consultantsmentioning

confidence: 99%

“…Previous studies have used a fast time-resolved imaging technique to visualize the transient microbubbles [1][2][3]. In this method, a laser pulse was applied to heat the particles and a second laser pulse, delayed in time relative to the first, was used to illuminate the sample for stroboscopic image capture.…”

Section: Collaborators/consultantsmentioning

confidence: 99%

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Mechanism of Ultrashort Pulse Laser Injury to the RPE (Retinal Pigment Epithelium)

Lin¹

2003

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Section: Significancesupporting

confidence: 79%

Section: Collaborators/consultantsmentioning

confidence: 99%

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Mechanism of Ultrashort Pulse Laser Injury to the RPE (Retinal Pigment Epithelium)

Lin¹

2003

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“…This phenomenon is central to a number of processes including eye surgery, selective killing of cells by irradiation of incorporated absorbing particles, 1 steam cleaning of surfaces, 2 and laser desorption mass spectrometry experiments of large biomolecules. [3][4][5] To understand the separation of a fluid from a laserheated substrate, optical reflectance and scattering studies 6,7 as well as photoacoustic experiments 8 have been performed.…”

Section: Introductionmentioning

confidence: 99%

Explosive Boiling of Water Films Adjacent to Heated Surfaces: A Microscopic Description

et al. 2001

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Molecular dynamics simulations are used to investigate the separation of water films adjacent to a hot metal surface. The simulations clearly show that the water layers nearest the surface overheat and undergo explosive boiling. For thick films, the expansion of the vaporized molecules near the surface forces the outer water layers to move away from the surface. These results are of interest for mass spectrometry of biological molecules, steam cleaning of surfaces, and medical procedures.

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“…In the nanosecond (ns) to microsecond pulse regime, these high temperatures and pressures can lead to melanosome microcavitation, producing spatially confined mechanical damage to the surrounding tissue. [1][2][3][4][5][6][7][8][9][10] A better understanding of the melanosome temperature required for cavitation, and of the subsequent mechanical effects, is necessary to determine the cellular damage mechanisms associated with melanosome microcavitation. The temperature required for cavitation, in the absence of irradiation, is known as the nucleation temperature and has been estimated by various groups, using temperature-dependent microcavitation data for ns-pulse exposures at 532 nm.…”

Section: Introductionmentioning

confidence: 99%

Temperature dependence of nanosecond laser pulse thresholds of melanosome and microsphere microcavitation

et al. 2016

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Abstract. Melanosome microcavitation is the threshold-level retinal pigment epithelium (RPE) damage mechanism for nanosecond (ns) pulse exposures in the visible and near-infrared (NIR). Thresholds for microcavitation of isolated bovine RPE melanosomes were determined as a function of temperature (20 to 85°C) using single ns laser pulses at 532 and 1064 nm. Melanosomes were irradiated using a 1064-nm Q-switched Nd:YAG (doubled for 532-nm irradiation). For comparison to melanosome data, a similar temperature (20 to 65°C) dependence study was also performed for 532 nm, ns pulse exposures of black polystyrene microbeads. Results indicated a decrease in the microcavitation average radiant exposure threshold with increasing sample temperature for both 532-and 1064-nm single pulse exposures of melanosomes and microbeads. Threshold data and extrapolated nucleation temperatures were used to estimate melanosome absorption coefficients in the visible and NIR, and microbead absorption coefficients in the visible, indicating that melanin is a better absorber of visible light than black polystyrene. The NIR melanosome absorption coefficients ranged from 3713 cm −1 at 800 nm to 222 cmat 1319 nm. These data represent the first temperature-dependent melanosome microcavitation study in the NIR and provide additional information for understanding melanosome microcavitation threshold dependence on wavelength and ambient temperature. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 UnportedLicense. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Selective cell killing by microparticle absorption of pulsed laser radiation

Cited by 104 publications

References 11 publications

Mechanism of Ultrashort Pulse Laser Injury to the RPE (Retinal Pigment Epithelium)

Mechanism of Ultrashort Pulse Laser Injury to the RPE (Retinal Pigment Epithelium)

Explosive Boiling of Water Films Adjacent to Heated Surfaces: A Microscopic Description

Temperature dependence of nanosecond laser pulse thresholds of melanosome and microsphere microcavitation

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