Selective degradation of transcripts during meiotic maturation of mouse oocytes

Su, You-Qiang; Sugiura, Koji; Woo, Yong; Wigglesworth, Karen; Kamdar, Sonya J.; Affourtit, Jason P.; Eppig, John J.

doi:10.1016/j.ydbio.2006.09.008

Cited by 291 publications

(289 citation statements)

References 73 publications

(84 reference statements)

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“…On the basis of the GO annotation (of the identified known ESTs), it was determined that ,94% of transcripts detected in our library were involved in crucial molecular events associated with oocyte and preimplantation embryonic development in different mammalian species such as bovine (Dalbie's-Tran and Mermillod 2003;Fair et al, 2007;Ghanem et al, 2007;Salilew-Wondim et al, 2007), ovine (Baillet et al, 2008), human (Assou et al, 2006) and mice (Su et al, 2007). Identified genes were also classified as oocyte-specific genes involved in folliculogenesis, oogenesis, fertilization, zygotic genome activation and preimplantation embryo development (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

Rajput

Kumar

Roy

et al. 2013

Animal

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A buffalo oocyte-specific subtracted cDNA library was constructed to identify exclusively or preferentially oocyte-expressed genes. The library represented an enriched population of transcripts obtained from oocytes of diverse ovarian follicular origin and at different stages of in vitro maturation. A total of 1173 high-quality sequences of oocyte-specific genes were clustered into 645 unique sequences, out of which 65.76% were represented as singlets and 34.26% as contig expressed sequence tags (ESTs; clusters). Analysis of sequences revealed that 498 of these sequences were identified as a known sequence in mammalian species including buffalo, 103 as uncharacterized ESTs and 44 unknown sequences including 1 novel EST, so far not reported in any species. Gene ontology annotation classified these sequences into functional categories of cellular events and biological processes associated with oocyte competence. Expression status of the isolated unknown ESTs confirmed that many of these are expressed in oocytes exclusively and in others preferentially, some in excess of 80-fold greater in comparison with a variety of somatic tissues. The isolated novel EST was detected to be expressed exclusively in oocytes and testicular cells only. To our knowledge, this is the first report giving a detailed transcriptome account of oocyte-expressed genes in buffalo. This study will provide important information on the physiological control of oocyte development, as well as many questions yet to be addressed on the reproductive process of buffalo.

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Section: Discussionmentioning

confidence: 99%

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

Rajput

Kumar

Roy

et al. 2013

Animal

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“…The sequences for the Rec8, SMC3, SMC1β, STAG3 and Rpl19 primer pairs are listed in Table 1. Calculations of the relative fold changes of Rec8, SMC3, SMC1β and STAG3 were performed using the 2 -ΔΔCt method, as described previously [27]. All experiments were repeated three times, and the values shown are the mean±SEM.…”

Section: Serum Hormone Measurementsmentioning

confidence: 99%

The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice

Cheng

Hou

et al. 2013

J Assist Reprod Genet

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Purpose The present study examined the effect of aging on female reproductive potential. Methods Six-week-old and 9-month-old CD1 mice were referred to as the 'young' and 'aged' groups, respetively. Oocytes were collected after superovulation, and their viability were compared using parthenogenetic activation. The aneuploidy of the oocytes (MII) was assessed using chromosome spread, and the whole ovarian follicle number was counted using an unbiased stereological method. Serum hormone levels were measured using the radio-immunity method, and the expression of the Cohesin subunit genes in the oocytes (GV) were assessed using RT-PCR. Results The mean number of recovered (25.8 vs. 16.2; P<0.05) and live oocytes (24.0 vs. 11.73; P <0.01) per head in the young-mice group (6-week-old) was significantly higher than that of the aged group (9-month-old). The aneuploidy rate of the ovulated oocytes in the aged group was significantly higher than that of the young group (36.8 % vs. 10 %; P<0.01), and the rate of blastocyst formation in the young group (85.23 %) was significantly higher than that of the aged group (81.2 %; P <0.05). The number of primordial follicles (the oocyte pool) per ovary in the aged group was significantly decreased compared with the young group (330±33.51 vs. 2079.6±420.70; P<0.01), and the level of AMH in the aged group was significantly higher than that of the young group (4.66±0.11 ng/ml vs. 4.07±0.18 ng/ml; P<0.01). Conclusions We propose that maternal aging significantly reduces the oocyte pool, superovulation efficiency and developmental potential and increases the oocyte aneuploidy rate.

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“…En Xenopus el estado inactivo de los RNAm maternos está mediado por la proteína CPEB, que interacciona al mismo tiempo tanto con la caja CPE en el extremo 3´UTR del RNAm materno, como al factor de inicio de la traducción 4E (eIF4E), que al no unirse al complejo de inicio de la traducción 4F (eIF4F) inhibe la traducción; por otro lado, la interacción CPEB/CPE ocasiona que el complejo proteico CCR4/NOT empiece a degradar la cola de poliadeninas (29) . Una vez que la meiosis se reactiva y el proceso de maduración del ovocito inicia, la activación de los RNAm maternos se da a través del proceso de poliadenilación citoplásmica (33) . Durante la maduración meiótica, la cinasa aurora (Eg2) fosforila la CPEB; de esta forma fosforilada CPEB se une a la proteína Maskin, por lo que se libera eIF4E, el cual puede ir y conformar el complejo eIF4F, dando lugar a la traducción (34) .…”

Section: Inactivación/activación De Los Rnam Maternosunclassified

“…Once meiosis is reactivated and oocyte the maturation process begin, the maternal mRNA activation occurs through the process of cytoplasmic polyadenylation (33) . During meiotic maturation, the Aurora kinase (Eg2) phosphorylates the PCEB; in this way phosphorylated PCEB joins Maskin protein, so it releases eIF4E, which can go and create the eIF4F complex, giving rise to the translation (34) .…”

Section: Inactivación/activación De Los Rnam Maternosmentioning

confidence: 99%

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

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Selective degradation of transcripts during meiotic maturation of mouse oocytes

Cited by 291 publications

References 73 publications

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

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