Selective Deposition and Self-Assembly of Triblock Copolymers into Matrix Arrays for Membrane Protein Production

Andreasson-Ochsner, Mirjam; Fu, Zhikang; May, Sylvia; Xiu, Low Ying; Nallani, Madhavan; Sinner, Eva‐Kathrin

doi:10.1021/la2038087

Cited by 14 publications

(11 citation statements)

References 37 publications

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“…The earlier experiment reported the synthesis of a diblock copolymer polybutadiene‐polyethylene oxide ([PBD] 21 –[PEO] 12 that was successfully applied for expression and reconstitution of α‐hemolysin as well as claudin‐2 into proteopolymerosome (Nallani et al, 2011). Later, selective deposition and self‐assembly of triblock copolymers (polymethyloxazoline‐polydimethylsiloxane‐polymethyloxazoline [PMOXA‐PDMS‐PMOXA], (ABA)) were demonstrated for fabrication of matrix arrays for dopamine receptor 2 (DRD2) membrane protein expression, producing membrane protein array for diagnostics or drug discovery purposes (Andreasson‐Ochsner et al, 2012). Likewise, production and integration of DRD2 into PMOXA (20) –PDMS (54) –PMOXA (20) and PBd (22) –PEO (13) block copolymer vesicles are used for ligand‐ and antibody‐binding assays (Figure 7e; May et al, 2013).…”

Section: Cfps: Latest Platforms and Applications In Biotechnology Andmentioning

confidence: 99%

Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Ayoubi‐Joshaghani

Dianat‐Moghadam

Seidi

et al. 2020

Biotech & Bioengineering

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Thanks to the synthetic biology, the laborious and restrictive procedure for producing a target protein in living microorganisms by biotechnological approaches can now experience a robust, pliant yet efficient alternative. The new system combined with lab‐on‐chip microfluidic devices and nanotechnology offers a tremendous potential envisioning novel cell‐free formats such as DNA brushes, hydrogels, vesicular particles, droplets, as well as solid surfaces. Acting as robust microreactors/microcompartments/minimal cells, the new platforms can be tuned to perform various tasks in a parallel and integrated manner encompassing gene expression, protein synthesis, purification, detection, and finally enabling cell‐cell signaling to bring a collective cell behavior, such as directing differentiation process, characteristics of higher order entities, and beyond. In this review, we issue an update on recent cell‐free protein synthesis (CFPS) formats. Furthermore, the latest advances and applications of CFPS for synthetic biology and biotechnology are highlighted. In the end, contemporary challenges and future opportunities of CFPS systems are discussed.

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Section: Cfps: Latest Platforms and Applications In Biotechnology Andmentioning

confidence: 99%

Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Ayoubi‐Joshaghani

Dianat‐Moghadam

Seidi

et al. 2020

Biotech & Bioengineering

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“…The date also show how the function incorporation values were measured and how M n (which can be quantiied using NMR) is related to M w as PDI = M w / M n . This table does not include the incorporation studies that do not include block copolymerprotein interactions, such as cell-free expression systems [73][74][75], nanopores [76,77], encapsulation in hydrophobic interior [6], hydrogel approaches [78,79], and non-amphiphilic polymers [80]. Due to this restriction on the data, the table showcases the results that were made available by Wolfgang Meier and coworkers implementing PMOXA-PDMS-PMOXA triblock copolymers.…”

Section: Figures 1 and 2)mentioning

confidence: 99%

Interactions between Aquaporin Proteins and Block Copolymer Matrixes

Abdelrasoul¹,

Doan²,

Lohi³

2017

Biomimetic and Bioinspired Membranes for New Frontiers in Sustainable Water Treatment Technology

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This chapter continues to further expand its focus on aquaporins (AQPs) by ofering a general outline on how the AQPs block copolymers, and polymer support structures can interrelate and such connections can be comprehensively classiied and deined. The irst section of the overview will consider the relationship between block copolymers and AQPs. It will also examine the general membrane protein integration into block copolymers, since this can cause AQP-block copolymer complexes in vesicular (proteopolymersomes) as well as in planar forms. The majority of considerations taken into account during AQP incorporation come from the research conducted in relation to the process of incorporating other types of membrane proteins. This chapter includes an overview of the various characterization methodologies needed for the study of proteopolymersomes, as well as freeze-fracture transmission electron microscopy (FF-TEM), luorescence correlation spectroscopy (FCS), small-angle X-ray scatering (SAXS), and stopped-low light scatering (SFLS). The research data presented in this chapter emphasizes the fact that a successful process of membrane fabrication requires the integration of reconstituted AQPs into a suitable supporting matrix formation.Keywords: aquaporin proteins, block copolymer, matrix, vesicular, membrane protein Assessing aquaporin proteins and block copolymer matrixes interactionsMost research performed on membrane protein inclusion has been conducted primarily with lipids as the host matrix components (original proteoliposomes publication on the subject came out in 1971) [1]. Since then, polymer-based incorporation process has received increased atention and the earliest proteopolymersomes publication emerged in 2000 [2]. The initial work stages in this research area concentrated on the inclusion of membranespanning proteins, such as membrane-bound ion channels (ATPases) and bacteriorhodopsin incorporation into polymethyloxazoline-polydimethylsiloxane-polymethyloxazoline (PMOXA-PDMS-PMOXA) triblock copolymer bilayers occurring in planar [3] or vesicular forms [4][5][6]. It is quite fascinating that the membrane proteins may be functionally incorporated into polymeric bilayers (e.g., based on PMOXA-PDMS-PMOXA) that occur up to 10 times thicker than their lipidic counterparts [7]. Moreover, researchers have observed proteopolymersomes with protein density values that drastically surpassed those of proteoliposomes [8]. Research on these phenomena has helped to establish a theoretical approach for generalized membrane protein incorporation into the amphiphilic structures. This methodology is based on the notion that the eiciency of the membrane protein incorporation process relies on its hydrophobicity potential and its coupling capacity to the host membrane is directly connected to the hydrophobic mismatch parameters. In order to reduce this mismatch dynamic, the method calls for the host membrane to be deformed in such a way that it matches the hydrophobic length parameter of the membrane protein's transm...

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“…Fortunately, unlike natural materials, a rich variety of polymers with different functional groups may be able to provide a solution to technical hurdles (e.g., hydrophobic mismatch). Examples are the report of Meier’s group on protein activity in a thick polymer membrane (~10 nm) [72,73] and a report on the successful incorporation of dopamine receptor D2 into a thick planar membrane [74]. Under simplified conditions, Pata and Dan predicted that the concentration of proteins decreases with increasing hydrophobic mismatch, and perturbation decay length of the polymer membrane is relatively longer than that of lipid bilayers [75].…”

Section: Effects Of Polymer Characteristics On the Stability And Fmentioning

confidence: 99%

Recent Progress in Advanced Nanobiological Materials for Energy and Environmental Applications

Choi

Montemagno

2013

Materials

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In this review, we briefly introduce our efforts to reconstruct cellular life processes by mimicking natural systems and the applications of these systems to energy and environmental problems. Functional units of in vitro cellular life processes are based on the fabrication of artificial organelles using protein-incorporated polymersomes and the creation of bioreactors. This concept of an artificial organelle originates from the first synthesis of poly(siloxane)-poly(alkyloxazoline) block copolymers three decades ago and the first demonstration of protein activity in the polymer membrane a decade ago. The increased value of biomimetic polymers results from many research efforts to find new applications such as functionally active membranes and a biochemical-producing polymersome. At the same time, foam research has advanced to the point that biomolecules can be efficiently produced in the aqueous channels of foam. Ongoing research includes replication of complex biological processes, such as an artificial Calvin cycle for application in biofuel and specialty chemical production, and carbon dioxide sequestration. We believe that the development of optimally designed biomimetic polymers and stable/biocompatible bioreactors would contribute to the realization of the benefits of biomimetic systems. Thus, this paper seeks to review previous research efforts, examine current knowledge/key technical parameters, and identify technical challenges ahead.

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Selective Deposition and Self-Assembly of Triblock Copolymers into Matrix Arrays for Membrane Protein Production

Cited by 14 publications

References 37 publications

Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Interactions between Aquaporin Proteins and Block Copolymer Matrixes

Recent Progress in Advanced Nanobiological Materials for Energy and Environmental Applications

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