Selective desensitization of jasmonate- and pH-dependent signaling in the induction of benzophenanthridine biosynthesis in cells of Eschscholzia californica

Färber, Katrin; Schumann, Brigitte; Miersch, Otto; Roos, Werner

doi:10.1016/s0031-9422(02)00562-9

Cited by 34 publications

(33 citation statements)

References 35 publications

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“…These pH shifts have been visualized in intact cells by confocal pH topography (Roos et al, 1998;Fä rber et al, 2003). Similar proton fluxes can be triggered by adding ;1 mM LPC to vacuoles in situ (i.e., cells with permeabilized plasma membrane).…”

Section: Introductionmentioning

confidence: 85%

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The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

et al. 2006

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Section: Introductionmentioning

confidence: 85%

“…Under such conditions, the cellular content of BBEmRNA peaks between 3 and 5 h (Figure 1). The increase of alkaloid content starts off between 8 and 10 h after elicitor contact (Fä rber et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

et al. 2006

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“…One cascade is JA-dependent and responds to high elicitor concentrations. The other is JA-independent and is triggered by low elicitor concentrations (Fä rber et al, 2003). The JA-independent pathway involves Ga proteins that interact and activate phospholipase A2, which leads to a transient efflux of protons from the vacuole and subsequent activation of other cytoplasmic components (Heinze et al, 2007;Viehweger et al, 2006).…”

Section: Signal Transductionmentioning

confidence: 99%

Opium poppy and Madagascar periwinkle: model non‐model systems to investigate alkaloid biosynthesis in plants

Facchini¹,

Luca²

2008

The Plant Journal

228

134

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SummaryAlkaloids represent a large and diverse group of compounds that are related by the occurrence of a nitrogen atom within a heterocyclic backbone. Unlike other types of secondary metabolites, the various structural categories of alkaloids are unrelated in terms of biosynthesis and evolution. Although the biology of each group is unique, common patterns have become apparent. Opium poppy (Papaver somniferum), which produces several benzylisoquinoline alkaloids, and Madagascar periwinkle (Catharanthus roseus), which accumulates an array of monoterpenoid indole alkaloids, have emerged as the premier organisms used to study plant alkaloid metabolism. The status of these species as model systems results from decades of research on the chemistry, enzymology and molecular biology responsible for the biosynthesis of valuable pharmaceutical alkaloids. Opium poppy remains the only commercial source for morphine, codeine and semisynthetic analgesics, such as oxycodone, derived from thebaine. Catharanthus roseus is the only source for the anti-cancer drugs vinblastine and vincristine. Impressive collections of cDNAs encoding biosynthetic enzymes and regulatory proteins involved in the formation of benzylisoquinoline and monoterpenoid indole alkaloids are now available, and the rate of gene discovery has accelerated with the application of genomics. Such tools have allowed the establishment of models that describe the complex cell biology of alkaloid metabolism in these important medicinal plants. A suite of biotechnological resources, including genetic transformation protocols, has allowed the application of metabolic engineering to modify the alkaloid content of these and related species. An overview of recent progress on benzylisoquinoline and monoterpenoid indole alkaloid biosynthesis in opium poppy and C. roseus is presented.

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“…Tentative evidence arose from the induction of secondary biosynthetic enzymes independent of an oxidative burst and/or elevated levels of jasmonates (van der Fits et al, 2000;Färber et al, 2003;Pauw et al, 2004b). Transient shifts of the intracellular pH that preceded the induction of secondary biosynthetic genes were earlier considered as potential components of signal pathways toward the expression of secondary biosynthesis (Kuchitsu et al, 1997;Roos et al, 1998;Roos, 2000;Shibuya and Minami, 2001).…”

mentioning

confidence: 99%

“…In contrast to the LPC/pH signal path, the jasmonatetriggered expression of alkaloid biosynthesis, which can be evoked in the same cell culture by higher elicitor concentrations, involves elements of the hypersensitive response (see above) but neither includes nor requires vacuolar/cytosolic pH shifts (Roos et al, 1998;Färber et al, 2003;Viehweger et al, 2006;Angelova et al, 2010).…”

mentioning

confidence: 99%

The Vacuolar Proton-Cation Exchanger EcNHX1 Generates pH Signals for the Expression of Secondary Metabolism in Eschscholzia californica

et al. 2015

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Cell cultures of Eschscholzia californica react to a fungal elicitor by the overproduction of antimicrobial benzophenanthridine alkaloids. The signal cascade toward the expression of biosynthetic enzymes includes (1) the activation of phospholipase A2 at the plasma membrane, resulting in a peak of lysophosphatidylcholine, and (2) a subsequent, transient efflux of vacuolar protons, resulting in a peak of cytosolic H + . This study demonstrates that one of the Na + /H + antiporters acting at the tonoplast of E. californica cells mediates this proton flux. Four antiporter-encoding genes were isolated and cloned from complementary DNA (EcNHX1-EcNHX4). RNA interference-based, simultaneous silencing of EcNHX1, EcNHX3, and EcNHX4 resulted in stable cell lines with largely diminished capacities of (1) sodium-dependent efflux of vacuolar protons and (2) elicitor-triggered overproduction of alkaloids. Each of the four EcNHX genes of E. californica reconstituted the lack of Na + -dependent H + efflux in a Dnhx null mutant of Saccharomyces cerevisiae. Only the yeast strain transformed with and expressing the EcNHX1 gene displayed Na + -dependent proton fluxes that were stimulated by lysophosphatidylcholine, thus giving rise to a net efflux of vacuolar H + . This finding was supported by three-dimensional protein homology models that predict a plausible recognition site for lysophosphatidylcholine only in EcNHX1. We conclude that the EcNHX1 antiporter functions in the elicitor-initiated expression of alkaloid biosynthetic genes by recruiting the vacuolar proton pool for the signaling process.

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Selective desensitization of jasmonate- and pH-dependent signaling in the induction of benzophenanthridine biosynthesis in cells of Eschscholzia californica

Cited by 34 publications

References 35 publications

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

Opium poppy and Madagascar periwinkle: model non‐model systems to investigate alkaloid biosynthesis in plants

The Vacuolar Proton-Cation Exchanger EcNHX1 Generates pH Signals for the Expression of Secondary Metabolism in Eschscholzia californica

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