Selective Detection of Dopamine Using Glassy Carbon Electrode Modified by a Combined Electropolymerized Permselective Film of Polytyramine and Polypyrrole‐1‐propionic Acid

Abstract

A sensitive and selective electrochemical method for the determination of dopamine using a combined electropolymerized permselective film of polytyramine and polypyrrole‐1‐propionic acid on a glassy carbon (GC) electrode was developed. The formation of a “layer‐by‐layer” film has allowed for selective detection of dopamine in the presence of 3,4‐dihydroxyphenylalanine (L‐DOPA), DOPAC, ascorbic acid, uric acid, epinephrine and norepinephrine. The modified electrodes exhibited a detection limit of 100 nM with linearity ranging from 5×10−6 to 5×10−5 M. No cleaning step was required during the course of repeated measurement.

Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison

“…Hence, both types of sensor chips can be re-used with good reproducibility more than 15 times, with relative standard deviation (% RSD) of 6%, and 7%, respectively. The low % RSD values suggest the high surface stability of the strongly electrodeposited Pty film and chemisorpted MCH-SAM on the sensor chip surfaces [32,36]. Similar results regarding reusability capacity, of about 12 to 20 times, for a Pty-ssDNA-modified sensor chip in capacitive biosensor measurements were previously recorded [6,37].…”

A solid state sensor based polytyramine film modified electrode for the determination of dopamine and ascorbic acid in a moderately acidic solution

“…The thickness and the amount of Pty deposited on the electrode surface are directly affected by the number of potential cycles and scan rates. For instance, the accumulation of the polymeric film increases with scan rates of 10 mV s À1 or lower resulting with a non-homogeneous film possessing considerable pine-holes [44]. Increasing the scan rates and limiting the number of reversible cycles may produce a uniform and thin polymeric layer.…”

A dopamine sensor based on a methoxypolyethylene glycol polymer covalently modified glassy carbon electrode

“…7 However, a major obstacle encountered with the detection of DA in real biological samples is the coexistence of some interferences, of which ascorbic acid (AA) is of particular importance due to the fact that AA is oxidized at potentials close to that of DA on conventional solid electrodes, resulting in an overlapping voltammetric response, and this species is present in most biological samples at much higher concentrations. 5,18,19 To overcome this problem, a variety of materials for the preparation of the modied electrodes have been reported in the majority of literature, such as materials based on nanoparticles modied electrodes, [20][21][22][23][24][25][26][27][28][29][30][31] polymercoated electrodes [32][33][34][35][36][37] and composite materials modied electrodes. [38][39][40][41][42] Among them, polymer lm modied electrodes are extensively used because such modied electrodes seem to be stable and can provide a sufficient number of active centers for wide application in electrocatalytic processes.…”