Selective Detection of Phosphotyrosine in the Presence of Various Phosphate‐Containing Biomolecules with the Aid of a Terbium(III) Complex

Abstract

Finding phosphotyrosine: Phosphotyrosine (either in its monomeric form or as a component of oligopeptides) was selectively detected by using luminescence in the presence of a specialized terbium(III) complex, without any signals from nonphosphorylated tyrosine, phosphoserine, phosphothreonine, nucleotides, or nucleic acids. Tyrosine phosphorylation could clearly be distinguished even in the presence of single‐stranded RNA.

Selective detection of tyrosine-containing proximally phosphorylated motifs using an antenna-free Tb3+ luminescent sensor

“…However, as the pTAY peptide did not induce significant signal enhancement, it suggested that direct phosphorylation on the Y antenna may be required. 6 Consistent with previous studies 18,19,21 we observed no Tb 3+ luminescence enhancement in response to some biologically relevant phospho-anions including ATP, AMP, and PPi. Although the signal was not observed, Tb 3+ was expected to bind the pSpS phosphorylated site.…”

“…Hamachi et al and Molecular Probes Inc.) have achieved significant progress in the detection of mono-phosphorylated peptide and protein sequences. [18][19][20][21][22][23] The signal selectivity of Tb 3+ for pY arises from the luminescence sensitization of the Tb 3+ ion (Scheme 1). [14][15][16][17] Although transition metal-based luminescent phospho-protein sensors were demonstrated to be effective and versatile in their applications, they cannot be readily optimized to recognize among the residues that are phosphorylated, i.e.…”

Binuclear Terbium(III) Complex as a Probe for Tyrosine Phosphorylation

“…We have previously demonstrated by using Tb-DOTAM that the benzene ring of pTyr in the target peptides can be used as an antenna to enhance the emission from the Tb III center (see Figure 1). [16] Of Tyr, Ser, Thr, and their phosphorylated products, only pTyr possesses both the notable antenna effect (benzene ring) and sufficient binding activity towards the Tb III complex (phosphate group). Thus, the selectivities (i) and (ii) are fulfilled.…”

A Label-free Time-resolved Luminescent Platform for Sensitive Endonuclease V Detection Based on Exonuclease III Regulated DNA-Tb3+ Luminescence

“…27 Therefore, combining with Tb 3+ , dGMP and G-rich DNA were recruited to detect small molecules and nucleic acid. 27,28 Interestingly, to our surprise, a remarkable distinction emerged when the Tb 3+ emission is enhanced by the same concentrations of guanine in different forms. As shown in Table 1, dGMP can result in a nonlinear enhancement of Tb 3+ emission with the increase of the concentrations of dGMP, but other dNTPs (N = C, A or T) cannot.…”

“…It has thus drawn the attention of analysts for developing applications for its use in sensors, especially in high sensitivity analysis and biochemistry fields. 28 In this work, we interestingly found that, though both guaninerich (G-rich) ssDNA and dGMP could greatly enhance the longlived luminescence of Tb 3+ , 27,28 the efficiencies of enhancement were considerably different. Inspired by this phenomenon, integrating the excellent properties of ligand-sensitized Tb 3+ TRL and signal regulation technique of Exonuclease III (Exo III), a convenient and outstanding performance EndoV detection strategy was developed.…”