Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe

Abstract

Quantitation of superoxide radical (O 2–·) production at the site of radical generation remains challenging. A simple method to detect nanomolar to micromolar levels of superoxide radical in aqueous solution has been developed and optimized. This method is based on the efficient trapping of O2–· using a novel fluorescent probe (2‐chloro‐1,3‐dibenzothiazolinecyclohexene), coupled with a spectra character‐signaling increase event. A high‐specificity and high‐sensitivity fluorescent probe was synthesized in‐house and used to image O2–· in living cells. Better selectivity for O2–· over competing cellular reactive oxygen species and some biological compounds illustrates the advantages of our method. Under optimal conditions, the linear calibration range for superoxide anion radicals was 5.03 × 10−9−3.33 × 10−6 m. The detection limit was 1.68 × 10−9 m. Fluorescence images of probe‐stained macrophages stimulated with 4β‐phorbol 12‐myristate 13‐acetate were obtained successfully using a confocal laser scanning microscope.

Functional roles of superoxide and hydrogen peroxide generated by mitochondrial DNA mutation in regulating tumorigenicity of HepG2 cells

“…CAT has also been established as an antioxidant that can scavenge extracellular H 2 O 2 and inhibited the proliferation of HER‐2/Neutransformed rat‐1 fibroblasts . Tiron, a specific scavenger for O 2 •− , could be used as an anticancer agent in preclinical models. Notwithstanding, the specific outcomes with these ROS scavengers, antioxidants continued to be of key interest for chemoprevention.…”

“…•− , 29 could be used as an anticancer agent in preclinical models. Notwithstanding, the specific outcomes with these ROS scavengers, antioxidants continued to be of key interest for chemoprevention.…”

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

“…The in vitro detection of O 2 •− has been realized over the past decade, and more recently the sensing in biological samples has been achieved . For instance, a fluorescent probe based on a vanillin scaffold functionalized with 8‐amino quinolone was synthesized to detect O 2 •− .…”

A Hemicyanine‐Embedded Diphenylselenide‐Containing Probe “HemiSe” in which SePh2 Stays Reduced for Selective Detection of Superoxide in Living Cells

“…[21] However,t hese methods are hindered by certaindisadvantages. by the An group), [25] hydroethidine [26] and boronate (e.g. On the other hand, organic fluorescent probes have become more andm ore reliable for monitoring bioactive molecules andh ave transformedf rom a corpus of sporadicreports to an important and large methodologic biological fieldo fs tudy that interfaces with am ultitude of other criteria;i tb ears major advantages such as good selectivity,h igh sensitivity,a nd real-timed etection.…”