Selective determination of famotidine in human plasma by high performance liquid chromatography in alkaline media with solid phase extraction

Abstract

Selective determination of famotidine in human plasma by high performance liquid chromatography in alkaline media with solid phase extraction A new method is described for the determination of famotidine by solid phase extraction from alkalinized human plasma followed by reversed phase (RP) HPLC in acetonitrile/alkaline buffer with molsidomine as an internal standard. Different acetonitrile/ aqueous buffer mobile phases as well as various RP columns were used. Alkaline medium allowed the limit of quantitation to be lowered to 5 ng/mL of plasma as the famotidine gives more intense absorption at about 286 nm (at pH values higher than 7). Moreover, work in alkaline media and at this wavelength is highly selective as peaks corresponding to impurities present in most samples are well separated. A method using a mildly alkaline mobile phase (acetonitrile/10 mM phosphate with 10 mM 1-heptanesulphonic acid, pH 7.5) was successfully used for determination of famotidine in human plasma in a pharmacokinetic study.

Instrumental analysis of some anti-ulcer drugs in different matrices

“…[20] RP-HPLC analysis of famotidine in human plasma was reported after solid phase extraction. [52] Another RP-HPLC methods were developed for the determination of famotidine in pharmaceutical products. [25,53,54] A quantitative method using silica gel high performance thin layer chromatography (HPTLC) plates with fluorescent indicator, automated sample application, and ultraviolet (UV) absorption videodensitometry was developed for the determination of famotidine tablets.…”

Quantitative determination of famotidine in human maternal plasma, umbilical cord plasma and urine using high‐performance liquid chromatography–mass spectrometry

“…The major disadvantage of prior famotidine assays by reversephase HPLC is its poor retention by the column because of its extreme polarity in acidic conditions. Attempts to improve the retention of famotidine on the column to achieve the desired separation from the early-eluting endogenous peaks have included the HPLC column switch technique (Zhong and Yeh, 1998) Determination of famotidine in human plasma and urine using LC-MS an ion-pair reagent (Dowling and Frye, 1999;Zarzhi et al, 1998), normal-phase HPLC (Zhong et al, 2011) and alkaline mobile phase (Anzenbacherova et al, 2003). After considering the compatibility of the HLPC method and the MS detector, the alkaline mobile phase was selected.…”