Selective determination of hydrogen peroxide by adduct formation with a dinuclear iron(iii) complex and flow injection analysis/tandem mass spectrometry

Abstract

A highly selective method for the determination of hydrogen peroxide is presented. In a flow injection analysis (FIA) instrument, the analyte is brought into contact with a dinuclear heptadentate iron(III) complex. The formation of the peroxide adduct is quantified using electrospray tandem mass spectrometry (ESI-MS/MS). Selected reaction monitoring (SRM) based on the transition from the triply charged peroxide adduct with m/z = 251.2 to the triply charged fragment ion of m/z = 240.5 is performed. The limit of detection for hydrogen peroxide is 10(-7) mol dm(-3), limit of quantification is 3 x 10(-7) mol dm(-3), and a linear range of 2.5 decades starting at the limit of quantification is observed.

A Novel ESIPT Phthalimide-Based Fluorescent Probe for Quantitative Detection of H2O2

“…Currently, there is a lot of analytical approaches for detection of H 2 O 2 , such as the electrochemical method, 10 colorimetric method, 11 mass spectrometry, 12 high-performance liquid chromatography. 13 Though the above methods work well for detection of H 2 O 2 , most of them require destruction of cells or tissues and complicated preparation.…”