There are various factors involved in the transmission of video over internet. These factors have direct impact on the quality of the transmitted video. The underlying video codec, encapsulation method and protocol are the key players which affect the quality of video transmission on a network. Up to our knowledge various research studies have been carried out to analyze the impact of these factors independently on the quality of video transmission however their combined effect on video transmission is not yet done. In this paper we characterize the impact of video codec, encapsulation methods and transmission protocols of video streaming setup, while keeping in view the percent frame loss as the main investigating parameter. The results obtained from the experiments carried out for the key investigating factor are presented in the results section of this paper.To be more precise, it was observed that the wmv1, mpeg2, m-jpeg codecs, asf encapsulation and rtp protocol performs best.