Selective enrichment of N‐linked glycopeptides and glycans by using a dextran‐modified hydrophilic material

Chen, Linlin; Ding, Dianyuan; Sheng, Qianying; Yu, Long; Liu, Xiuping; Liang, Xinmiao

doi:10.1002/jssc.201700995

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…In a comparison of boronate affinity, Zwitterionic (ZIC)‐HILIC and PGC methods for glycopeptide enrichment, boronic acid chemistry was found to preferentially capture glycopeptides with high mannose glycans, ZIC‐HILIC enriched most N ‐glycopeptides and did not show significant preference during enrichment, and PGC was found to be not suitable for separating glycopeptides with a long amino acid sequence (Xue et al, 2018). In another study, identical results were obtained with Sepharose, PGC and dextran‐bonded silica for the separation of complex N ‐glycans (Chen, Ding, et al, 2018). Sun, Xiong, et al (2018) have developed a D‐allose@SiO 2 matrix and found that enrichment of sialylated glycopeptides was notably higher than with the commercially available Sepharose CL‐6B or TiO 2 resins.…”

Section: Studies On Specific Carbohydrate Typessupporting

confidence: 54%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

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Section: Studies On Specific Carbohydrate Typessupporting

confidence: 54%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

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“…Hydrophilic interaction chromatography (HILIC) is considered as a mixed-mode mechanism, mainly based on hydrophilic partitioning and other interactions such as electrostatic interactions, dipole interactions, adsorption, hydrogen bonding, and so on . With excellent hydrophilicity and similar polyhydroxy structure, several polysaccharide-modified materials have been widely employed in HILIC mode to enrich glycopeptides, such as glucose, maltose, , cellulose, glucose-6-phosphate, chitosan, , sepharose, and dextran. , There are some advantages, such as high glycosylation coverage and unbiased recognition ability for glycopeptides, as well as excellent compatibility with MS analysis. However, there are still some difficulties to be improved.…”

mentioning

confidence: 99%

“…19 With excellent hydrophilicity and similar polyhydroxy structure, several polysaccharidemodified materials have been widely employed in HILIC mode to enrich glycopeptides, such as glucose, 20 maltose, 21,22 cellulose, 23 glucose-6-phosphate, 24 chitosan, 25,26 sepharose, 27 and dextran. 28,29 There are some advantages, such as high glycosylation coverage and unbiased recognition ability for glycopeptides, as well as excellent compatibility with MS analysis. However, there are still some difficulties to be improved.…”

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confidence: 99%

Hydrophilic Nanocomposite Functionalized by Carrageenan for the Specific Enrichment of Glycopeptides

et al. 2019

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A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency. This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly-(ethylenimine) support (denoted as GO−PEI−Carr). Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics. The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides. Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio. A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500. Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples. In addition, GO−PEI−Carr exhibited a unique repeatability and stability even after enrichment of glycopeptides for 20 times. It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.

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“…Until now, MS has been extensively applied in proteomics analysis with the advantages of rapid analysis speed and high sensitivity [11,12]. However, there are still many obstacles in the direct identification of target analytes by MS, owing to the complexity of biological samples, the low abundance, and ionization efficiency of glycopeptides [13,14]. Thus, efficiently enriching glycopeptides from complex biological samples before MS analysis is necessary [15].…”

Section: Introductionmentioning

confidence: 99%

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

Wang

et al. 2022

J of Separation Science

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In this work, a novel porous bifunctionalized composite material was synthesized via a simple method. Gold nanoparticles are uniformly dispersed on the surface of the biomimetic honeycomb chitosan membrane through the interaction between amino and Au, and then cysteine and glutathione are successfully grafted onto the surface of the Au by the Au‐S bond. The modification of cysteine and glutathione makes this bifunctionalized composite material have significant advantages of superhydrophilicity and small steric hindrance simultaneously. This material manifests excellent property in glycopeptides enrichment, with high selectivity (1:5000), low detection limit (0.1 fmol·μL–1), high recovery rate (99.4 ± 0.5%), and good repeatability. In addition, with the help of nano‐flow liquid chromatography tandem mass spectrometry, this composite achieved excellent performance in efficiently enriching glycopeptides in the serum of healthy people and nasopharyngeal carcinoma's disease patient. More excitingly, further gene ontology analysis of molecular function and biological process indicated that 41 original glycoproteins of the identified glycopeptides from serum of nasopharyngeal carcinoma's disease patient significantly partake in numerous cancer‐associated events, including protease binding, calcium ion binding, enzyme binding, extracellular matrix organization, cellular response to tumor necrosis factor, and inflammatory response.

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Selective enrichment of N‐linked glycopeptides and glycans by using a dextran‐modified hydrophilic material

Cited by 9 publications

References 40 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Hydrophilic Nanocomposite Functionalized by Carrageenan for the Specific Enrichment of Glycopeptides

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

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