Selective Entropy Gain-Driven Adsorption of Nanospheres onto Spherical Bacteria Endows Photodynamic Treatment with Narrow-Spectrum Activity

Yang, Binqian; Gao, Feng; Li, Zhiyuan; Li, Mingyang; Chen, Liang; Guan, Yong; Liu, Gang; Yang, Lihua

doi:10.1021/acs.jpclett.0c00287

Cited by 13 publications

(9 citation statements)

References 56 publications

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“…coli K12W3110, this recognition mechanism could not be explained by electrostatic interaction alone. It has been reported that nanoprobe adhesion was not induced by electrostatic interaction only …”

Section: Resultsmentioning

confidence: 99%

Boronic Acid-Based Dendrimers with Various Surface Properties for Bacterial Recognition with Adjustable Selectivity

Mikagi

Manita

Tsuchido

et al. 2022

ACS Appl. Bio Mater.

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The need for a selective bacterial recognition method is evident to overcome the global problem of antibiotic resistance. Even though researchers have focused on boronic acid-based nanoprobes that immediately form boronate esters with saccharides at room temperature, the mechanism has not been well studied. We have developed boronic acid-modified poly(amidoamine) (PAMAM) dendrimers with various surface properties to investigate the mechanism of bacterial recognition. The boronic acid-based nanoprobes showed selectivity toward strains, species, or a certain group of bacteria by controlling their surface properties. Our nanoprobes showed selectivity toward Gram-positive bacteria or Escherichia coli K12W3110 without having to modify the boronic acid recognition sites. The results were obtained in 20 min and visible to the naked eye. Selectivity toward Gram-positive bacteria was realized through electrostatic interaction between the bacterial surface and the positively charged nanoprobes. In this case, the recognition target was lipoteichoic acid on the bacterial surface. On the other hand, pseudo-zwitterionic nanoprobes showed selectivity for E. coli K12W3110, indicating that phenylboronic acid did not recognize the outermost O-antigen on the lipopolysaccharide layer. Boronic acid-based nanoprobes with optimized surface properties are expected to be a powerful clinical tool to recognize multidrug-resistant strains or highly pathogenic bacteria.

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Section: Resultsmentioning

confidence: 99%

Boronic Acid-Based Dendrimers with Various Surface Properties for Bacterial Recognition with Adjustable Selectivity

Mikagi

Manita

Tsuchido

et al. 2022

ACS Appl. Bio Mater.

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“…Furthermore, it is reported that the efficient adherence of antibacterial agent to the bacterial membrane may induce bacteria cells apoptosis. [44][45][46] Therefore, detailed bactericidal mechanism of the FePN SAzyme against different bacterial strains could be unveiled by the FE-SEM images (Figure 7g and Figure S18, Supporting Information). After FePN SAzyme or 808 nm laser treatment alone, typically rod and spherical shaped were observed in E. coli and S. aureus bacteria respectively, accompanied with smooth and intact cell walls.…”

Section: Resultsmentioning

confidence: 99%

A Biofilm Microenvironment‐Activated Single‐Atom Iron Nanozyme with NIR‐Controllable Nanocatalytic Activities for Synergetic Bacteria‐Infected Wound Therapy

Hua

Zhang

et al. 2021

Adv Healthcare Materials

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Biofilm microenvironment (BME)-activated antimicrobial agents display great potential for improved biofilm-related infection therapy because of their superior specificities and sensitivities, effective eliminations, and minimal side effects. Herein, BME-activated Fe-doped polydiaminopyridine nanofusiform-mediated single-atom nanozyme (FePN SAzyme) is presented for photothermal/chemodynamic synergetic bacteria-infected wound therapy. The photothermal therapy (PTT) function of SAzyme can be specifically initiated by the high level of H 2 O 2 and further accelerated through mild acid within the inflammatory environment through "two-step rocket launching-like" process. Additionally, the enhanced chemodynamic therapy (CDT) for the FePN SAzyme can also be endowed by producing hydroxyl radicals through reacting with H 2 O 2 and consuming glutathione (GSH) of the BME, thereby contributing to more efficient synergistic therapeutic effect. Meanwhile, FePN SAzyme could catalyze biofilm-overexpressed H 2 O 2 decomposing into O 2 and overcome the hypoxia of biofilm, which significantly enhances the susceptibility of biofilm and increases the synergistic efficacy. Most importantly, the synergistic therapy of bacterial-induced infection diseases can be switched on by the internal and external stimuli simultaneously, resulting in minimal nonspecific damage to healthy tissue. These remarkable characteristics of FePN SAzyme not only develop an innovative strategy for the BME-activated combination therapy but also open a new avenue to explore other nanozyme-involved nanoplatforms for bacterial biofilm infections.

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“…The binding of negatively charged nanoparticles to the cell surface (Figure E) can be due to one or a combination of two mechanisms. A first one is based on electrostatic interactions between the negatively charged nanoparticles and cationic charges on the cell membrane. − A second mechanism that can promote binding of negatively charged nanoparticles onto the cell membrane is entropy gain-driven depletion. − …”

Section: Resultsmentioning

confidence: 99%

Covalent and Noncovalent Conjugation of Degradable Polymer Nanoparticles to T Lymphocytes

et al. 2021

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Cells are attractive as carriers that can help to enhance control over the biodistribution of polymer nanomedicines. One strategy to use cells as carriers is based on the cell surface immobilization of the nanoparticle cargo. While a range of strategies can be used to immobilize nanoparticles on cell surfaces, only limited effort has been made to investigate the effect of these surface modification chemistries on cell viability and functional properties. This study has explored seven different approaches for the immobilization of poly(lactic acid) (PLA) nanoparticles on the surface of two different T lymphocyte cell lines. The cell lines used were human Jurkat T cells and CD4 + T EM cells. The latter cells possess blood−brain barrier (BBB) migratory properties and are attractive for the development of cell-based delivery systems to the central nervous system (CNS). PLA nanoparticles were immobilized either via covalent active ester−amine, azide−alkyne cycloaddition, and thiol−maleimide coupling, or via noncovalent approaches that use lectin−carbohydrate, electrostatic, or biotin−NeutrAvidin interactions. The cell surface immobilization of the nanoparticles was monitored with flow cytometry and confocal microscopy. By tuning the initial nanoparticle/cell ratio, T cells can be decorated with up to ∼185 nanoparticles/cell as determined by confocal microscopy. The functional properties of the nanoparticle-decorated cells were assessed by evaluating their binding to ICAM-1, a key protein involved in the adhesion of CD4 + T EM cells to the BBB endothelium, as well as in a two-chamber model in vitro BBB migration assay. It was found that the migratory behavior of CD4 + T EM cells carrying carboxylic acid-, biotin-, or Wheat germ agglutinin (WGA)-functionalized nanoparticles was not affected by the presence of the nanoparticle payload. In contrast, however, for cells decorated with maleimide-functionalized nanoparticles, a reduction in the number of migratory cells compared to the nonmodified control cells was observed. Investigating and understanding the impact of nanoparticle−cell surface conjugation chemistries on the viability and properties of cells is important to further improve the design of cell-based nanoparticle delivery systems. The results of this study present a first step in this direction and provide first guidelines for the surface modification of T cells, in particular in view of their possible use for drug delivery to the CNS.

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Selective Entropy Gain-Driven Adsorption of Nanospheres onto Spherical Bacteria Endows Photodynamic Treatment with Narrow-Spectrum Activity

Cited by 13 publications

References 56 publications

Boronic Acid-Based Dendrimers with Various Surface Properties for Bacterial Recognition with Adjustable Selectivity

Boronic Acid-Based Dendrimers with Various Surface Properties for Bacterial Recognition with Adjustable Selectivity

A Biofilm Microenvironment‐Activated Single‐Atom Iron Nanozyme with NIR‐Controllable Nanocatalytic Activities for Synergetic Bacteria‐Infected Wound Therapy

Covalent and Noncovalent Conjugation of Degradable Polymer Nanoparticles to T Lymphocytes

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