Selective extraction and analysis of pioglitazone in cow plasma using a molecularly imprinted polymer combined with ESI ion mobility spectrometry

Abstract

A method based on ESI ion mobility spectrometry as a detection technique after treatment with a molecularly imprinted polymer is described for the analysis of pioglitazone. In addition to the molecularly imprinted polymer separation methodology, the positive ion mobility spectrum and the reduced mobility values for pioglitazone are reported for the first time. The method was exhaustively validated in terms of sensitivity, imprinting factor, enrichment factor, and sorption capacity. A linear dynamic range of 0.10-20.00 μg/mL and an RSD below 6% were obtained for the analysis of this compound. The average recovery for the analysis of spiked samples was calculated to be about 91%. The method was also used to determine pioglitazone in cow plasma, and the results were compared with those obtained using HPLC. The satisfactory results evidence a convenient method for the analysis of the target compound in real samples without using any additional derivatization methods.

High‐performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications

“…Two substance mixtures consisting of 24 pesticides and 18 amino acids are used for the characterization of the IM detector. The following compounds are contained in the pesticide mixture (Supporting Information: Table S1): diquatdibromide(1), aldicarbsulfoxide(2), imidacloprid(3), aldicarbsulfone(4), methomyl(5), oxamyl(6), chloridazone (7), metoxurone(8), 3-hydroxycarbofurane (9), aldicarb(10), monurone (11), propoxur (12), diurone (13), chlortolurone (14), carbofuran (15), carbaryl (16), methabenzthiazurone(17), monolinurone (18), isoproturone (19), metobromurone (20), metazachlor (21), methiocarb (22), linurone(23) and metolachlor (24). The amino acid mixture is comprised of the following compounds: DL-methionine (Met), DL-valine (Val), L-glutamine (Gln), glutamic acid (Glu), glycine (Gly), L-histidine (His), L-alanine (Ala), L-arginine (Arg), L-aspartic acid (Asp), L-asparagine (Asn), L-cysteine (Cys), L-isoleucine (Ile), L-leucine (Leu), L-phenylalanine (Phe), L-proline (Pro), L-serine (Ser), L-threonine (Thr), L-tyrosine (Tyr) and L-tryptophan (Trp).…”

“…Substances can be characterized by their IM as they are identified by their mass in MS. Another similarity to the latter is the possibility to implement different ionization sources such as ESI and APCI.ESI-IM spectrometry was first demonstrated in 1972 [2] and was developed more systematically in the 1990s [3]. The technique allows the investigation of a wide range of substance classes [4][5][6], and can be employed either as a standalone spectrometer (at atmospheric pressure) [7] or in combination with MS (at reduced pressure < 10 mbar or at…”

Induced mathematical filtration as an innovative strategy for discrimination and estimation of glycemic control drugs in fixed dose combination

“…Analysis of PIO was achieved using different methods and reported in the literature [ 19 ]. The recently developed techniques included spectrophotometry [ 20 , 21 , 22 ], chromatography including TLC [ 23 ] and HPLC [ 24 , 25 ].…”

Rapid analysis of pesticide residues in drinking water samples by dispersive solid‐phase extraction based on multiwalled carbon nanotubes and pulse glow discharge ion source ion mobility spectrometry

“…IMS is well suited for large‐scale, trace‐level investigation and monitoring programs where sophisticated laboratory instrument is not available . A number of literatures have demonstrated the applications of IMS in analysis of explosives , drugs , pharmaceuticals , Chinese medicines , pesticides , and other agricultural or industrial contaminants .…”